Gene description for Pafah1b1
Gene name platelet-activating factor acetylhydrolase, isoform 1b, subunit 1
Gene symbol Pafah1b1
Other names/aliases LIS-1
Lis1
MMS10-U
Mdsh
Ms10u
Pafaha
Species Mus musculus
 Database cross references - Pafah1b1
ExoCarta ExoCarta_18472
Vesiclepedia VP_18472
Entrez Gene 18472
UniProt P63005  
 Pafah1b1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pafah1b1
Molecular Function
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 IDA
    microtubule binding GO:0008017 ISO
    identical protein binding GO:0042802 IPI
    protein-containing complex binding GO:0044877 ISO
    dynein intermediate chain binding GO:0045505 IEA
    dynein intermediate chain binding GO:0045505 ISO
    protein heterodimerization activity GO:0046982 ISS
    microtubule plus-end binding GO:0051010 IBA
    phosphoprotein binding GO:0051219 IPI
    dynein complex binding GO:0070840 IBA
    dynein complex binding GO:0070840 IEA
    dynein complex binding GO:0070840 ISO
Biological Process
    establishment of mitotic spindle orientation GO:0000132 IBA
    establishment of mitotic spindle orientation GO:0000132 IEA
    establishment of mitotic spindle orientation GO:0000132 ISO
    microtubule cytoskeleton organization GO:0000226 IGI
    microtubule cytoskeleton organization GO:0000226 IMP
    ameboidal-type cell migration GO:0001667 IMP
    acrosome assembly GO:0001675 IMP
    neuron migration GO:0001764 IGI
    neuron migration GO:0001764 IMP
    neuron migration GO:0001764 ISO
    positive regulation of cytokine-mediated signaling pathway GO:0001961 IMP
    microtubule-based process GO:0007017 ISO
    nuclear migration GO:0007097 IBA
    nuclear migration GO:0007097 IEA
    nuclear migration GO:0007097 ISO
    JNK cascade GO:0007254 IGI
    chemical synaptic transmission GO:0007268 IMP
    germ cell development GO:0007281 IBA
    neuroblast proliferation GO:0007405 IMP
    learning or memory GO:0007611 IMP
    retrograde axonal transport GO:0008090 IBA
    retrograde axonal transport GO:0008090 IDA
    adult locomotory behavior GO:0008344 IMP
    adult locomotory behavior GO:0008344 ISO
    protein secretion GO:0009306 IMP
    negative regulation of neuron projection development GO:0010977 IEA
    negative regulation of neuron projection development GO:0010977 ISO
    lipid catabolic process GO:0016042 IEA
    cell migration GO:0016477 IMP
    stem cell division GO:0017145 IEA
    stem cell division GO:0017145 ISO
    transmission of nerve impulse GO:0019226 IMP
    corpus callosum morphogenesis GO:0021540 IEA
    corpus callosum morphogenesis GO:0021540 ISO
    hippocampus development GO:0021766 IGI
    hippocampus development GO:0021766 IMP
    layer formation in cerebral cortex GO:0021819 IGI
    cerebral cortex neuron differentiation GO:0021895 IEA
    cerebral cortex neuron differentiation GO:0021895 ISO
    cerebral cortex development GO:0021987 IGI
    cerebral cortex development GO:0021987 IMP
    cerebral cortex development GO:0021987 ISO
    actin cytoskeleton organization GO:0030036 IMP
    microtubule organizing center organization GO:0031023 IBA
    microtubule organizing center organization GO:0031023 IEA
    microtubule organizing center organization GO:0031023 ISO
    osteoclast development GO:0036035 IMP
    reelin-mediated signaling pathway GO:0038026 IMP
    positive regulation of embryonic development GO:0040019 IMP
    establishment of planar polarity of embryonic epithelium GO:0042249 IMP
    regulation of GTPase activity GO:0043087 IMP
    cortical microtubule organization GO:0043622 IMP
    positive regulation of axon extension GO:0045773 IEA
    positive regulation of axon extension GO:0045773 ISO
    positive regulation of mitotic cell cycle GO:0045931 IEA
    positive regulation of mitotic cell cycle GO:0045931 ISO
    negative regulation of JNK cascade GO:0046329 IGI
    vesicle transport along microtubule GO:0047496 IBA
    vesicle transport along microtubule GO:0047496 IGI
    brain morphogenesis GO:0048854 IBA
    brain morphogenesis GO:0048854 IEA
    brain morphogenesis GO:0048854 ISO
    modulation of chemical synaptic transmission GO:0050804 IDA
    modulation of chemical synaptic transmission GO:0050804 IMP
    neuromuscular process controlling balance GO:0050885 IMP
    neuromuscular process controlling balance GO:0050885 ISO
    microtubule sliding GO:0051012 IEA
    nuclear membrane disassembly GO:0051081 IMP
    positive regulation of cellular component organization GO:0051130 IMP
    establishment of localization in cell GO:0051649 IMP
    establishment of centrosome localization GO:0051660 IEA
    establishment of centrosome localization GO:0051660 ISO
    maintenance of centrosome location GO:0051661 IMP
    auditory receptor cell development GO:0060117 IMP
    positive regulation of dendritic spine morphogenesis GO:0061003 IMP
    regulation of microtubule cytoskeleton organization GO:0070507 IMP
    cochlea development GO:0090102 IMP
    microtubule cytoskeleton organization involved in establishment of planar polarity GO:0090176 IMP
    myeloid leukocyte migration GO:0097529 IMP
    regulation of postsynapse organization GO:0099175 IDA
    regulation of postsynapse organization GO:0099175 IMP
    radial glia-guided pyramidal neuron migration GO:0140650 IGI
    interneuron migration GO:1904936 IMP
Subcellular Localization
    astral microtubule GO:0000235 IEA
    astral microtubule GO:0000235 ISO
    kinetochore GO:0000776 IBA
    kinetochore GO:0000776 IEA
    kinetochore GO:0000776 ISO
    nuclear envelope GO:0005635 IBA
    nuclear envelope GO:0005635 ISO
    nuclear envelope GO:0005635 ISS
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IDA
    centrosome GO:0005813 ISO
    kinesin complex GO:0005871 IEA
    kinesin complex GO:0005871 ISO
    microtubule associated complex GO:0005875 IBA
    microtubule associated complex GO:0005875 IDA
    microtubule associated complex GO:0005875 ISO
    cytoplasmic microtubule GO:0005881 IBA
    cell cortex GO:0005938 IEA
    cell cortex GO:0005938 ISO
    1-alkyl-2-acetylglycerophosphocholine esterase complex GO:0008247 ISO
    1-alkyl-2-acetylglycerophosphocholine esterase complex GO:0008247 ISS
    microtubule cytoskeleton GO:0015630 IDA
    axon GO:0030424 ISO
    growth cone GO:0030426 ISO
    cell leading edge GO:0031252 IDA
    motile cilium GO:0031514 IDA
    nuclear membrane GO:0031965 IEA
    vesicle GO:0031982 IEA
    vesicle GO:0031982 ISO
    stereocilium GO:0032420 IDA
    neuron projection GO:0043005 IBA
    neuronal cell body GO:0043025 IBA
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
    perinuclear region of cytoplasm GO:0048471 IDA
    central region of growth cone GO:0090724 IEA
    central region of growth cone GO:0090724 ISO
    non-motile cilium GO:0097730 IDA
    Schaffer collateral - CA1 synapse GO:0098685 IDA
    Schaffer collateral - CA1 synapse GO:0098685 IMP
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified Pafah1b1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pafah1b1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PAFAH1B1 5048
Affinity Capture-MS Homo sapiens
2 EDIL3 10085
Affinity Capture-MS Homo sapiens
3 HSPA5 3309
Affinity Capture-MS Homo sapiens
4 CCSER2 54462
Affinity Capture-MS Homo sapiens
5 Map1b 17755
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
6 PLS3 5358
Affinity Capture-MS Homo sapiens
7 SLC25A12 8604
Affinity Capture-MS Homo sapiens
8 Bicd2  
Co-fractionation Mus musculus
9 Zwint  
Co-fractionation Mus musculus
10 PAFAH1B3 5050
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 Nup85  
Co-fractionation Mus musculus
12 PAFAH1B1  
Affinity Capture-Western Bos taurus
13 Zfp276 57247
Co-fractionation Mus musculus
14 DYNC1I2 1781
Affinity Capture-MS Homo sapiens
15 PAFAH1B2 5049
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 PPP1CA 5499
Affinity Capture-MS Homo sapiens
17 GAK 2580
Affinity Capture-MS Homo sapiens
18 Kctd13  
Affinity Capture-MS Mus musculus
19 ARID4B  
Affinity Capture-MS Homo sapiens
20 CENPF 1063
Affinity Capture-MS Homo sapiens
21 Clasp2  
Co-fractionation Mus musculus
22 IMPACT 55364
Affinity Capture-MS Homo sapiens
23 DIXDC1  
Affinity Capture-MS Homo sapiens
24 Ndel1  
Affinity Capture-Western Mus musculus
Co-purification Mus musculus
25 ZNF616  
Affinity Capture-MS Homo sapiens
26 Nde1  
Reconstituted Complex Mus musculus
27 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
28 NELFCD 51497
Affinity Capture-MS Homo sapiens
29 Dync1h1 13424
Affinity Capture-Western Mus musculus
30 PSMA5 5686
Affinity Capture-MS Homo sapiens
31 DYNC1LI1 51143
Affinity Capture-MS Homo sapiens
32 NDE1 54820
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 IPO4 79711
Affinity Capture-MS Homo sapiens
34 Pafah1b1 18472
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
35 CEP135  
Affinity Capture-MS Homo sapiens
36 TMOD3 29766
Affinity Capture-MS Homo sapiens
37 DYNLRB1 83658
Affinity Capture-MS Homo sapiens
38 NDEL1 81565
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 PPP1R18 170954
Affinity Capture-MS Homo sapiens
40 Lnx1  
Affinity Capture-MS Mus musculus
41 CALD1 800
Affinity Capture-MS Homo sapiens
42 Reln 19699
Phenotypic Enhancement Mus musculus
43 Apc  
Co-fractionation Mus musculus
44 HECTD4 283450
Affinity Capture-MS Homo sapiens
45 ATXN3 4287
Affinity Capture-MS Homo sapiens
46 PRSS21  
Affinity Capture-MS Homo sapiens
47 FSCN1 6624
Affinity Capture-MS Homo sapiens
48 Dab1  
Phenotypic Enhancement Mus musculus
49 HS6ST2 90161
Affinity Capture-MS Homo sapiens
50 Kif3b  
Co-fractionation Mus musculus
51 KATNB1  
Two-hybrid Homo sapiens
52 DYNC1LI2 1783
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Pafah1b1 is involved
PathwayEvidenceSource
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal IEA Reactome
Amplification of signal from the kinetochores IEA Reactome
Anchoring of the basal body to the plasma membrane IEA Reactome
AURKA Activation by TPX2 IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Centrosome maturation IEA Reactome
Cilium Assembly IEA Reactome
COPI-independent Golgi-to-ER retrograde traffic IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
G2/M Transition IEA Reactome
Golgi-to-ER retrograde transport IEA Reactome
Intra-Golgi and retrograde Golgi-to-ER traffic IEA Reactome
Loss of Nlp from mitotic centrosomes IEA Reactome
Loss of proteins required for interphase microtubule organization from the centrosome IEA Reactome
M Phase IEA Reactome
Membrane Trafficking IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Mitotic Spindle Checkpoint IEA Reactome
Organelle biogenesis and maintenance IEA Reactome
Recruitment of mitotic centrosome proteins and complexes IEA Reactome
Recruitment of NuMA to mitotic centrosomes IEA Reactome
Regulation of PLK1 Activity at G2/M Transition IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Vesicle-mediated transport IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here