Gene description for Khdrbs1
Gene name KH domain containing, RNA binding, signal transduction associated 1
Gene symbol Khdrbs1
Other names/aliases Sam68
p62
p68
Species Mus musculus
 Database cross references - Khdrbs1
ExoCarta ExoCarta_20218
Vesiclepedia VP_20218
Entrez Gene 20218
UniProt Q60749  
 Khdrbs1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Khdrbs1
Molecular Function
    RNA binding GO:0003723 IDA
    RNA binding GO:0003723 ISO
    RNA binding GO:0003723 ISS
    mRNA binding GO:0003729 IBA
    protein binding GO:0005515 IPI
    poly(A) binding GO:0008143 IBA
    poly(A) binding GO:0008143 ISO
    poly(U) RNA binding GO:0008266 ISO
    SH3 domain binding GO:0017124 IEA
    SH3 domain binding GO:0017124 ISO
    protein domain specific binding GO:0019904 ISO
    signaling adaptor activity GO:0035591 IDA
    signaling adaptor activity GO:0035591 ISO
    SH2 domain binding GO:0042169 IEA
    SH2 domain binding GO:0042169 ISO
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISO
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    protein tyrosine kinase binding GO:1990782 IPI
    protein tyrosine kinase binding GO:1990782 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 IBA
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 ISO
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 ISS
    mRNA processing GO:0006397 IEA
    cell surface receptor signaling pathway GO:0007166 IDA
    cell surface receptor signaling pathway GO:0007166 ISO
    spermatogenesis GO:0007283 IEA
    regulation of apoptotic process GO:0042981 IEA
    positive regulation of translational initiation GO:0045948 ISO
    positive regulation of translational initiation GO:0045948 ISS
    regulation of RNA export from nucleus GO:0046831 IDA
    positive regulation of RNA export from nucleus GO:0046833 ISO
    positive regulation of RNA export from nucleus GO:0046833 ISS
    regulation of mRNA splicing, via spliceosome GO:0048024 IDA
    T cell receptor signaling pathway GO:0050852 IEA
    T cell receptor signaling pathway GO:0050852 ISO
    regulation of cell cycle GO:0051726 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    membrane GO:0016020 ISO
    membrane GO:0016020 ISS
    protein-containing complex GO:0032991 ISO
    Grb2-Sos complex GO:0070618 IEA
    Grb2-Sos complex GO:0070618 ISO
 Experiment description of studies that identified Khdrbs1 in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Khdrbs1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Yes1 22612
Biochemical Activity Mus musculus
2 Fyn  
Biochemical Activity Mus musculus
3 Zbtb7b  
Affinity Capture-MS Mus musculus
4 Mettl21e  
Affinity Capture-MS Mus musculus
5 Ripk1  
Reconstituted Complex Mus musculus
6 Ncoa5  
Two-hybrid Mus musculus
7 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
8 Kctd13  
Affinity Capture-MS Mus musculus
9 Src 20779
Biochemical Activity Mus musculus
Biochemical Activity Mus musculus
10 Lyn 17096
Biochemical Activity Mus musculus
11 TRAF2 7186
Affinity Capture-Western Homo sapiens
12 Rasa1  
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
13 Atg16l1  
Affinity Capture-MS Mus musculus
14 Gtf2e2  
Two-hybrid Mus musculus
15 Khdrbs1 20218
Two-hybrid Mus musculus
Two-hybrid Mus musculus
16 Rbm39 170791
Two-hybrid Mus musculus
17 Otx2  
Affinity Capture-MS Mus musculus
18 RASA1 5921
Reconstituted Complex Homo sapiens
19 Kcnma1  
Affinity Capture-MS Mus musculus
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