Gene description for GBAS
Gene name glioblastoma amplified sequence
Gene symbol GBAS
Other names/aliases NIPSNAP2
Species Homo sapiens
 Database cross references - GBAS
ExoCarta ExoCarta_2631
Vesiclepedia VP_2631
Entrez Gene 2631
HGNC 4179
MIM 603004
UniProt O75323  
 GBAS identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
 Gene ontology annotations for GBAS
Molecular Function
    protein binding GO:0005515 IPI
    protein-macromolecule adaptor activity GO:0030674 IDA
Biological Process
    mitophagy GO:0000423 IDA
    oxidative phosphorylation GO:0006119 IMP
    mitochondrion organization GO:0007005 IMP
    positive regulation of high voltage-gated calcium channel activity GO:1901843 ISS
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISS
    mitochondrial outer membrane GO:0005741 IDA
    mitochondrial matrix GO:0005759 IDA
 Experiment description of studies that identified GBAS in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GBAS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Co-fractionation Homo sapiens
2 GABARAPL1 23710
Affinity Capture-MS Homo sapiens
3 HMOX1 3162
Affinity Capture-MS Homo sapiens
4 NPEPPS 9520
Affinity Capture-MS Homo sapiens
5 OSBPL10  
Affinity Capture-MS Homo sapiens
6 PTMA 5757
Co-fractionation Homo sapiens
7 PFKP 5214
Affinity Capture-MS Homo sapiens
8 RPA2 6118
Proximity Label-MS Homo sapiens
9 EBNA-LP  
Affinity Capture-MS
10 NDUFA4 4697
Affinity Capture-MS Homo sapiens
11 PDHB 5162
Co-fractionation Homo sapiens
12 PRKDC 5591
Affinity Capture-MS Homo sapiens
13 SF3B5 83443
Affinity Capture-MS Homo sapiens
14 SLC25A24 29957
Co-fractionation Homo sapiens
15 ATG13 9776
Affinity Capture-Western Homo sapiens
16 MAP1LC3B 81631
Affinity Capture-MS Homo sapiens
17 GNG12 55970
Co-fractionation Homo sapiens
18 PGK1 5230
Affinity Capture-MS Homo sapiens
19 COQ9  
Affinity Capture-MS Homo sapiens
20 COX7A2L 9167
Co-fractionation Homo sapiens
21 ADCY6 112
Cross-Linking-MS (XL-MS) Homo sapiens
22 IMPDH2 3615
Co-fractionation Homo sapiens
23 MARCH5  
Proximity Label-MS Homo sapiens
24 HNRNPU 3192
Co-fractionation Homo sapiens
25 ATP5O 539
Co-fractionation Homo sapiens
26 PGRMC2 10424
Co-fractionation Homo sapiens
27 SLC25A46 91137
Proximity Label-MS Homo sapiens
28 PHB 5245
Co-fractionation Homo sapiens
29 ATP5H 10476
Co-fractionation Homo sapiens
30 VWA8 23078
Proximity Label-MS Homo sapiens
31 MTA2 9219
Affinity Capture-MS Homo sapiens
32 LAMTOR1 55004
Co-fractionation Homo sapiens
33 PRNP 5621
Two-hybrid Homo sapiens
34 MDH2 4191
Proximity Label-MS Homo sapiens
35 JMJD6 23210
Affinity Capture-MS Homo sapiens
36 SAMM50 25813
Affinity Capture-MS Homo sapiens
37 RALA 5898
Co-fractionation Homo sapiens
38 ATP6V0C 527
Co-fractionation Homo sapiens
39 CDH1 999
Affinity Capture-MS Homo sapiens
40 CYB5A 1528
Co-fractionation Homo sapiens
41 APOL2 23780
Co-fractionation Homo sapiens
42 BTF3 689
Affinity Capture-MS Homo sapiens
43 GABARAP 11337
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 ACTN4 81
Co-fractionation Homo sapiens
45 NTRK1 4914
Affinity Capture-MS Homo sapiens
46 NCL 4691
Co-fractionation Homo sapiens
47 CYC1 1537
Co-fractionation Homo sapiens
48 RPN1 6184
Co-fractionation Homo sapiens
49 GANAB 23193
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
50 PML 5371
Co-fractionation Homo sapiens
51 COX7B 1349
Co-fractionation Homo sapiens
52 VDAC2 7417
Co-fractionation Homo sapiens
53 NIPSNAP1 8508
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
54 VCP 7415
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
55 PRKCSH 5589
Co-fractionation Homo sapiens
56 HLA-DPB1 3115
Affinity Capture-MS Homo sapiens
57 EFNA4  
Affinity Capture-MS Homo sapiens
58 HADHB 3032
Co-fractionation Homo sapiens
59 HADHA 3030
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
60 VAPB 9217
Co-fractionation Homo sapiens
61 NASP 4678
Co-fractionation Homo sapiens
62 CANX 821
Co-fractionation Homo sapiens
63 SLC4A1 6521
Co-fractionation Homo sapiens
64 XPO1 7514
Affinity Capture-MS Homo sapiens
65 EGLN3  
Affinity Capture-MS Homo sapiens
66 CTNNB1 1499
Co-fractionation Homo sapiens
67 SRSF1 6426
Co-fractionation Homo sapiens
68 PRKCZ 5590
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 ATP5F1 515
Co-fractionation Homo sapiens
71 NAP1L1 4673
Co-fractionation Homo sapiens
72 RCN1 5954
Co-fractionation Homo sapiens
73 LARS 51520
Affinity Capture-MS Homo sapiens
74 NXF1 10482
Affinity Capture-RNA Homo sapiens
75 PRKCI 5584
Affinity Capture-MS Homo sapiens
76 CRAT 1384
Affinity Capture-MS Homo sapiens
77 PHB2 11331
Co-fractionation Homo sapiens
78 ANKFY1 51479
Affinity Capture-MS Homo sapiens
79 RPA3 6119
Proximity Label-MS Homo sapiens
80 SLC3A2 6520
Co-fractionation Homo sapiens
81 ATP1B1 481
Co-fractionation Homo sapiens
82 AMOT 154796
Proximity Label-MS Homo sapiens
83 MFN2 9927
Proximity Label-MS Homo sapiens
84 HK1 3098
Affinity Capture-MS Homo sapiens
85 CD96  
Affinity Capture-MS Homo sapiens
86 COX2 4513
Co-fractionation Homo sapiens
87 HSPD1 3329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 PPP1R3B  
Affinity Capture-MS Homo sapiens
89 PFAS 5198
Affinity Capture-MS Homo sapiens
90 PA2G4 5036
Affinity Capture-MS Homo sapiens
91 TRIM5 85363
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
92 CLPP 8192
Proximity Label-MS Homo sapiens
93 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
94 Prkci  
Affinity Capture-MS Mus musculus
95 FOXO3  
Affinity Capture-MS Homo sapiens
96 AUH 549
Co-fractionation Homo sapiens
97 PARP1 142
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 ALDH9A1 223
Affinity Capture-MS Homo sapiens
99 PGRMC1 10857
Co-fractionation Homo sapiens
100 EIF3I 8668
Co-fractionation Homo sapiens
101 RUVBL2 10856
Co-fractionation Homo sapiens
102 WBP11  
Affinity Capture-MS Homo sapiens
103 LONP1 9361
Affinity Capture-MS Homo sapiens
104 KEAP1 9817
Affinity Capture-MS Homo sapiens
105 COX5A 9377
Co-fractionation Homo sapiens
106 GABARAPL2 11345
Affinity Capture-MS Homo sapiens
107 SQSTM1 8878
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 FKBP4 2288
Affinity Capture-MS Homo sapiens
109 COX8A  
Co-fractionation Homo sapiens
110 CAPRIN1 4076
Co-fractionation Homo sapiens
111 TMLHE 55217
Affinity Capture-MS Homo sapiens
112 TARS 6897
Affinity Capture-MS Homo sapiens
113 SFXN1 94081
Co-fractionation Homo sapiens
114 DDX39A 10212
Affinity Capture-MS Homo sapiens
115 NAMPT 10135
Affinity Capture-MS Homo sapiens
116 MAP1LC3A 84557
Affinity Capture-MS Homo sapiens
117 MOV10 4343
Affinity Capture-RNA Homo sapiens
118 EEF1D 1936
Co-fractionation Homo sapiens
119 ATP1A1 476
Co-fractionation Homo sapiens
120 KLHL22 84861
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
121 PTGES3 10728
Co-fractionation Homo sapiens
122 COX7C 1350
Co-fractionation Homo sapiens
123 CD44 960
Affinity Capture-MS Homo sapiens
124 WARS 7453
Affinity Capture-MS Homo sapiens
125 DCP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
126 MGST3 4259
Co-fractionation Homo sapiens
127 COX4I1 1327
Co-fractionation Homo sapiens
128 COX6C 1345
Co-fractionation Homo sapiens
View the network image/svg+xml



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