Gene description for CRAT
Gene name carnitine O-acetyltransferase
Gene symbol CRAT
Other names/aliases CAT1
Species Homo sapiens
 Database cross references - CRAT
ExoCarta ExoCarta_1384
Vesiclepedia VP_1384
Entrez Gene 1384
HGNC 2342
MIM 600184
UniProt P43155  
 CRAT identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CRAT
Molecular Function
    acyl-CoA oxidase activity GO:0003997 IDA
    carnitine O-acetyltransferase activity GO:0004092 IBA
    carnitine O-acetyltransferase activity GO:0004092 IDA
    carnitine O-acetyltransferase activity GO:0004092 TAS
    carnitine O-octanoyltransferase activity GO:0008458 IEA
Biological Process
    carnitine metabolic process, CoA-linked GO:0019254 IBA
    carnitine metabolic process, CoA-linked GO:0019254 IDA
    fatty acid beta-oxidation using acyl-CoA oxidase GO:0033540 IDA
    fatty acid beta-oxidation using acyl-CoA oxidase GO:0033540 TAS
    short-chain fatty acid metabolic process GO:0046459 IDA
    medium-chain fatty acid metabolic process GO:0051791 IDA
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 IEA
    peroxisome GO:0005777 IBA
    peroxisome GO:0005777 IDA
    peroxisomal matrix GO:0005782 TAS
    endoplasmic reticulum GO:0005783 IEA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified CRAT in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CRAT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NIPSNAP3A  
Affinity Capture-MS Homo sapiens
2 FBXL4 26235
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 CS 1431
Proximity Label-MS Homo sapiens
4 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
5 CHN1  
Co-fractionation Homo sapiens
6 MRPS2 51116
Affinity Capture-MS Homo sapiens
7 HSPA8 3312
Co-fractionation Homo sapiens
8 CAPZA2 830
Affinity Capture-MS Homo sapiens
9 METTL20  
Affinity Capture-MS Homo sapiens
10 SLC25A10 1468
Affinity Capture-MS Homo sapiens
11 ACSM5 54988
Affinity Capture-MS Homo sapiens
12 SMAD4  
Affinity Capture-MS Homo sapiens
13 NDUFS7 374291
Affinity Capture-MS Homo sapiens
14 PTDSS1 9791
Affinity Capture-MS Homo sapiens
15 PLD5  
Affinity Capture-MS Homo sapiens
16 NPSR1  
Affinity Capture-MS Homo sapiens
17 METAP1D  
Affinity Capture-MS Homo sapiens
18 SLC25A20 788
Co-fractionation Homo sapiens
19 TRMU  
Affinity Capture-MS Homo sapiens
20 RGS3 5998
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 CBR4  
Affinity Capture-MS Homo sapiens
22 NUDT16 131870
Affinity Capture-MS Homo sapiens
23 SPATA2  
Affinity Capture-MS Homo sapiens
24 MYL10 93408
Affinity Capture-MS Homo sapiens
25 F12  
Affinity Capture-MS Homo sapiens
26 YARS2  
Affinity Capture-MS Homo sapiens
27 HINT2 84681
Affinity Capture-MS Homo sapiens
28 MB21D1  
Proximity Label-MS Homo sapiens
29 PEX5 5830
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
30 GCAT  
Affinity Capture-MS Homo sapiens
31 FTSJ2 29960
Affinity Capture-MS Homo sapiens
32 RASL10B  
Affinity Capture-MS Homo sapiens
33 ACOX1 51
Co-fractionation Homo sapiens
34 NQO2 4835
Co-fractionation Homo sapiens
35 PFDN5 5204
Affinity Capture-MS Homo sapiens
36 GBAS 2631
Affinity Capture-MS Homo sapiens
37 ACAA2 10449
Affinity Capture-MS Homo sapiens
38 COX4I1 1327
Affinity Capture-MS Homo sapiens
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