Gene description for SSU72
Gene name SSU72 RNA polymerase II CTD phosphatase homolog (S. cerevisiae)
Gene symbol SSU72
Other names/aliases HSPC182
PNAS-120
Species Homo sapiens
 Database cross references - SSU72
ExoCarta ExoCarta_29101
Vesiclepedia VP_29101
Entrez Gene 29101
HGNC 25016
UniProt Q9NP77  
 SSU72 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for SSU72
Molecular Function
    protein binding GO:0005515 IPI
    RNA polymerase II CTD heptapeptide repeat phosphatase activity GO:0008420 IBA
    RNA polymerase II CTD heptapeptide repeat phosphatase activity GO:0008420 IDA
    myosin phosphatase activity GO:0017018 IEA
    calmodulin-dependent protein phosphatase activity GO:0033192 IEA
    histone H2AXS140 phosphatase activity GO:0140791 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity GO:0180004 IEA
    RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity GO:0180005 IEA
    RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity GO:0180006 IEA
    RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity GO:0180007 IEA
    RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity GO:0180008 IEA
    MAP kinase serine/threonine phosphatase activity GO:1990439 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    termination of RNA polymerase II transcription GO:0006369 IBA
    co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway GO:0180010 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IDA
    mRNA cleavage and polyadenylation specificity factor complex GO:0005847 IBA
 Experiment description of studies that identified SSU72 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SSU72
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ACAT2 39
Co-fractionation Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 UBE2H 7328
Co-fractionation Homo sapiens
4 SMC3 9126
Affinity Capture-Western Homo sapiens
5 RAD21 5885
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 MAPK9 5601
Two-hybrid Homo sapiens
7 PPP1CB 5500
Co-fractionation Homo sapiens
8 CPSF1 29894
Affinity Capture-MS Homo sapiens
9 TPI1 7167
Co-fractionation Homo sapiens
10 SEC23IP 11196
Co-fractionation Homo sapiens
11 PCF11 51585
Proximity Label-MS Homo sapiens
12 TIPRL 261726
Co-fractionation Homo sapiens
13 UGGT1 56886
Co-fractionation Homo sapiens
14 PRM3  
Affinity Capture-MS Homo sapiens
15 FAS 355
Proximity Label-MS Homo sapiens
16 EN1  
Affinity Capture-MS Homo sapiens
17 THOP1 7064
Co-fractionation Homo sapiens
18 SYMPK 8189
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
19 SKP1 6500
Co-fractionation Homo sapiens
20 TRIO 7204
Co-fractionation Homo sapiens
21 EGFR 1956
Negative Genetic Homo sapiens
22 UIMC1  
Affinity Capture-MS Homo sapiens
23 CSTF2 1478
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
24 POLR2A 5430
Biochemical Activity Homo sapiens
Protein-peptide Homo sapiens
Co-crystal Structure Homo sapiens
25 DNPH1 10591
Co-fractionation Homo sapiens
26 HTT 3064
Co-fractionation Homo sapiens
27 NUDCD2 134492
Co-fractionation Homo sapiens
28 XPO1 7514
Co-fractionation Homo sapiens
29 CPSF2 53981
Affinity Capture-MS Homo sapiens
30 NCOA5  
Proximity Label-MS Homo sapiens
31 FOS 2353
Affinity Capture-MS Homo sapiens
32 VCL 7414
Co-fractionation Homo sapiens
33 CSTF3 1479
Affinity Capture-MS Homo sapiens
34 HIST2H2AA4 723790
Affinity Capture-MS Homo sapiens
35 PRDX6 9588
Co-fractionation Homo sapiens
36 STXBP2 6813
Co-fractionation Homo sapiens
37 LACTB2 51110
Co-fractionation Homo sapiens
38 ZNF638 27332
Proximity Label-MS Homo sapiens
39 KYNU  
Co-fractionation Homo sapiens
40 SPANXN4  
Affinity Capture-MS Homo sapiens
41 CDKN2AIP  
Proximity Label-MS Homo sapiens
42 PTGES3 10728
Co-fractionation Homo sapiens
43 ELP5 23587
Proximity Label-MS Homo sapiens
44 KRAS 3845
Negative Genetic Homo sapiens
45 NCAPG2 54892
Co-fractionation Homo sapiens
46 SMC1A 8243
Affinity Capture-Western Homo sapiens
47 HNRNPL 3191
Affinity Capture-MS Homo sapiens
48 EPB41L5 57669
Affinity Capture-MS Homo sapiens
49 MAPK8 5599
Two-hybrid Homo sapiens
50 CPSF6 11052
Affinity Capture-MS Homo sapiens
51 CAPN2 824
Co-fractionation Homo sapiens
52 CPSF3 51692
Affinity Capture-MS Homo sapiens
53 STAG2 10735
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
54 POU3F1  
Affinity Capture-MS Homo sapiens
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