Gene description for IFIT3
Gene name interferon-induced protein with tetratricopeptide repeats 3
Gene symbol IFIT3
Other names/aliases CIG-49
GARG-49
IFI60
IFIT4
IRG2
ISG60
P60
RIG-G
Species Homo sapiens
 Database cross references - IFIT3
ExoCarta ExoCarta_3437
Vesiclepedia VP_3437
Entrez Gene 3437
HGNC 5411
MIM 604650
UniProt O14879  
 IFIT3 identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Thymus 23844026    
 Gene ontology annotations for IFIT3
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    negative regulation of cell population proliferation GO:0008285 IDA
    response to virus GO:0009615 IMP
    negative regulation of apoptotic process GO:0043066 IDA
    defense response to virus GO:0051607 IBA
    antiviral innate immune response GO:0140374 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified IFIT3 in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for IFIT3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNRPB2 6629
Affinity Capture-MS Homo sapiens
2 APP 351
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
3 CYP2C8  
Two-hybrid Homo sapiens
4 YBX1 4904
Affinity Capture-MS Homo sapiens
5 HNRNPDL 9987
Affinity Capture-MS Homo sapiens
6 HNRNPD 3184
Affinity Capture-MS Homo sapiens
7 MAVS 57506
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 ISG15 9636
Affinity Capture-MS Homo sapiens
9 HNRNPL 3191
Affinity Capture-MS Homo sapiens
10 TRAF2 7186
Affinity Capture-Western Homo sapiens
11 SNRPE 6635
Affinity Capture-MS Homo sapiens
12 PABPC4 8761
Affinity Capture-MS Homo sapiens
13 RAD23A 5886
Two-hybrid Homo sapiens
14 IFNA14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 TMEM183A  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 APOE 348
Two-hybrid Homo sapiens
17 RBMX 27316
Affinity Capture-MS Homo sapiens
18 SNRPA1 6627
Affinity Capture-MS Homo sapiens
19 IFNA8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 ANXA1 301
Protein-peptide Homo sapiens
21 CDC37 11140
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
22 HNRNPA2B1 3181
Affinity Capture-MS Homo sapiens
23 SNRPA 6626
Affinity Capture-MS Homo sapiens
24 TRAF6 7189
Affinity Capture-Western Homo sapiens
25 HAUS1  
Two-hybrid Homo sapiens
26 TRADD 8717
Affinity Capture-Western Homo sapiens
27 TXN 7295
Affinity Capture-MS Homo sapiens
28 RNF32  
Two-hybrid Homo sapiens
29 SSB 6741
Affinity Capture-MS Homo sapiens
30 IFIT5 24138
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
31 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
32 HNRNPA3 220988
Affinity Capture-MS Homo sapiens
33 SF3B3 23450
Affinity Capture-MS Homo sapiens
34 DDX58 23586
Affinity Capture-Western Homo sapiens
35 TRAF3 7187
Affinity Capture-Western Homo sapiens
36 SNRPD2 6633
Affinity Capture-MS Homo sapiens
37 A2M 2
Two-hybrid Homo sapiens
38 IFIT1B  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
39 IFNA5  
Affinity Capture-MS Homo sapiens
40 TBK1 29110
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
41 FANCA  
Two-hybrid Homo sapiens
42 TPM1 7168
Two-hybrid Homo sapiens
43 LONP1 9361
Two-hybrid Homo sapiens
44 IFNA7  
Affinity Capture-MS Homo sapiens
45 IFNA2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 NCL 4691
Affinity Capture-MS Homo sapiens
47 IFIT2 3433
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
48 IFIT1 3434
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
49 SNRPB 6628
Affinity Capture-MS Homo sapiens
50 SNRNP70 6625
Affinity Capture-MS Homo sapiens
51 YBX3 8531
Affinity Capture-MS Homo sapiens
52 PABPC1 26986
Affinity Capture-MS Homo sapiens
53 MOV10 4343
Affinity Capture-MS Homo sapiens
54 HNRNPAB 3182
Affinity Capture-MS Homo sapiens
55 SAP25  
Affinity Capture-MS Homo sapiens
56 LVRN 206338
Two-hybrid Homo sapiens
57 SNRPD1 6632
Affinity Capture-MS Homo sapiens
58 HNRNPU 3192
Affinity Capture-MS Homo sapiens
59 SNRPC 6631
Affinity Capture-MS Homo sapiens
60 IFIT3 3437
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
61 CCDC67  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
62 SNRPF 6636
Affinity Capture-MS Homo sapiens
63 PHACTR4 65979
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 MAST1  
Two-hybrid Homo sapiens
65 IFNA21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 IKBKE 9641
Affinity Capture-Western Homo sapiens
67 PSEN2 5664
Two-hybrid Homo sapiens
68 ILF3 3609
Affinity Capture-MS Homo sapiens
69 SNRPD3 6634
Affinity Capture-MS Homo sapiens
70 ECSIT 51295
Two-hybrid Homo sapiens
71 TPM3 7170
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
72 DSP 1832
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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