Experiment description of studies that identified IGHM in sEVs

1 Experiment ID 46 MISEV standards ✔ EM|IEM Biophysical techniques ✔ HSC70|CD63|MHCII|CD81|CD86 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 17641064     Organism Homo sapiens Experiment description Exosomes with immune modulatory features are present in human breast milk. Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S" Journal name JIMMU Publication year 2007 Sample Breast milk Sample name Breast milk - Colostrum Isolation/purification methods Differential centrifugation Filtration Sucrose density gradient Flotation density 1.10-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [QSTAR] Western blotting Immunoelectron Microscopy FACS

2 Experiment ID 48 MISEV standards ✔ EM|IEM Biophysical techniques ✔ HSC70|CD63|CD86|MHCII Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 17641064     Organism Homo sapiens Experiment description Exosomes with immune modulatory features are present in human breast milk. Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S" Journal name JIMMU Publication year 2007 Sample Breast milk Sample name Breast milk - Mature milk Isolation/purification methods Differential centrifugation Filtration Sucrose density gradient Flotation density 1.10-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [QSTAR] Western blotting Immunoelectron Microscopy FACS

3 Experiment ID 494 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Osteoarthritic cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

4 Experiment ID 496 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Healthy cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

5 Experiment ID 497 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

6 Experiment ID 498 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

7 Experiment ID 21 MISEV standards ✔ EM|IEM Biophysical techniques ✔ Alix|TSG101|HSP70|CD63 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry    PubMed ID 19837982     Organism Homo sapiens Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215. Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson" Journal name MCP Publication year 2009 Sample Colorectal cancer cells Sample name LIM1215 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.10-1.12 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [Orbitrap] Western blotting

8 Experiment ID 52 MISEV standards ✔ EM Biophysical techniques ✔ HSP90|MHCII Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 14975938     Organism Homo sapiens Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions. Authors "Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN" Journal name AJRCMB Publication year 2004 Sample Malignant pleural effusions Sample name Pleural Fluid - Lung cancer Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [MALDI TOF] Western blotting

9 Experiment ID 126 MISEV standards ✘ Biophysical techniques ✔ GAPDH Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim" Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

10 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

11 Experiment ID 231 MISEV standards ✘ Biophysical techniques ✔ Alix|CD63|CD9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

12 Experiment ID 232 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

13 Experiment ID 233 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

14 Experiment ID 66 MISEV standards ✔ IEM Biophysical techniques ✔ TSG101|Alix|CD63|CD81 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19199708     Organism Homo sapiens Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT). Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR" Journal name JPR Publication year 2009 Sample Saliva Sample name Saliva Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy

Protein-protein interactions for IGHM

Protein Interactor ExoCarta ID Identification method PubMed Species 1 SYMPK 8189 Affinity Capture-MS Homo sapiens 2 BCKDK 10295 Affinity Capture-MS Homo sapiens 3 TK1 7083 Two-hybrid Homo sapiens 4 KRTCAP2 200185 Affinity Capture-MS Homo sapiens 5 GADD45A   Two-hybrid Homo sapiens 6 FASTKD5   Affinity Capture-MS Homo sapiens 7 ATM 472 Affinity Capture-MS Homo sapiens 8 EBF2   Affinity Capture-MS Homo sapiens 9 SLC27A6   Affinity Capture-MS Homo sapiens 10 SYVN1 84447 Affinity Capture-Western Homo sapiens 11 LTN1 26046 Affinity Capture-MS Homo sapiens 12 GCN1L1 10985 Affinity Capture-MS Homo sapiens 13 SOX2   Affinity Capture-MS Homo sapiens 14 FNDC5   Affinity Capture-MS Homo sapiens 15 UFD1L 7353 Two-hybrid Homo sapiens 16 DEGS1   Affinity Capture-MS Homo sapiens 17 WDR76   Affinity Capture-MS Homo sapiens 18 EGFL8   Affinity Capture-MS Homo sapiens 19 SEL1L 6400 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 20 SLC27A2 11001 Affinity Capture-MS Homo sapiens 21 SFXN4   Affinity Capture-MS Homo sapiens 22 TCEB3B   Affinity Capture-MS Homo sapiens 23 PHF11 51131 Affinity Capture-MS Homo sapiens 24 STARD3 10948 Affinity Capture-MS Homo sapiens 25 SNX27 81609 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 26 BTRC 8945 Affinity Capture-MS Homo sapiens 27 ESYT2 57488 Affinity Capture-MS Homo sapiens 28 UBQLN4 56893 Two-hybrid Homo sapiens 29 AGPAT4 56895 Affinity Capture-MS Homo sapiens 30 GSK3B 2932 Two-hybrid Homo sapiens 31 PDK1 5163 Affinity Capture-MS Homo sapiens 32 SPDYE7P   Affinity Capture-MS Homo sapiens 33 DDX31   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 34 GNG8   Affinity Capture-MS Homo sapiens 35 CYP2S1   Affinity Capture-MS Homo sapiens 36 MAPKAPK3 7867 Two-hybrid Homo sapiens 37 CERS3   Affinity Capture-MS Homo sapiens 38 TUBA1A 7846 Affinity Capture-MS Homo sapiens 39 GOLGA8A 23015 Affinity Capture-MS Homo sapiens 40 RNF213 57674 Affinity Capture-MS Homo sapiens 41 CSPG4 1464 Affinity Capture-MS Homo sapiens 42 CCR1   Affinity Capture-MS Homo sapiens 43 SREBF2 6721 Affinity Capture-MS Homo sapiens 44 UBE2U   Affinity Capture-MS Homo sapiens 45 PATL1 219988 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 46 YWHAQ 10971 Affinity Capture-MS Homo sapiens 47 SLC25A17 10478 Affinity Capture-MS Homo sapiens 48 TUBA1C 84790 Affinity Capture-MS Homo sapiens 49 HCN1   Affinity Capture-MS Homo sapiens 50 PPP2R2B 5521 Affinity Capture-MS Homo sapiens 51 CEP72   Two-hybrid Homo sapiens 52 ACSL4 2182 Affinity Capture-MS Homo sapiens 53 GJB5   Affinity Capture-MS Homo sapiens 54 ADCK1   Affinity Capture-MS Homo sapiens 55 HEATR6   Affinity Capture-MS Homo sapiens 56 STK3 6788 Affinity Capture-MS Homo sapiens 57 HSPA5 3309 Affinity Capture-MS Homo sapiens 58 CASC4 113201 Affinity Capture-MS Homo sapiens 59 ZAK 51776 Two-hybrid Homo sapiens 60 HUS1   Affinity Capture-MS Homo sapiens 61 AGPAT1 10554 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 62 HSD17B7 51478 Affinity Capture-MS Homo sapiens 63 FITM2   Affinity Capture-MS Homo sapiens 64 TMEM176B 28959 Affinity Capture-MS Homo sapiens 65 STK17B 9262 Affinity Capture-MS Homo sapiens 66 ARFGEF1 10565 Affinity Capture-MS Homo sapiens 67 LPCAT4   Affinity Capture-MS Homo sapiens 68 ACAD10   Affinity Capture-MS Homo sapiens 69 GHDC   Affinity Capture-MS Homo sapiens 70 ABHD12 26090 Affinity Capture-MS Homo sapiens 71 TUBA4A 7277 Affinity Capture-MS Homo sapiens 72 FANCA   Affinity Capture-MS Homo sapiens 73 ABHD6 57406 Affinity Capture-MS Homo sapiens 74 FBXO2 26232 Affinity Capture-MS Homo sapiens 75 SAV1   Affinity Capture-MS Homo sapiens 76 MTOR 2475 Affinity Capture-MS Homo sapiens 77 USP24 23358 Affinity Capture-MS Homo sapiens 78 STK4 6789 Affinity Capture-MS Homo sapiens 79 ATP7A 538 Affinity Capture-MS Homo sapiens 80 STX17 55014 Affinity Capture-MS Homo sapiens 81 SH3GL2   Two-hybrid Homo sapiens 82 SCCPDH 51097 Affinity Capture-MS Homo sapiens 83 CPT1A 1374 Affinity Capture-MS Homo sapiens 84 ZNF574   Affinity Capture-MS Homo sapiens 85 APP 351 Reconstituted Complex Homo sapiens 86 CIR1   Affinity Capture-MS Homo sapiens 87 ABHD16A 7920 Affinity Capture-MS Homo sapiens 88 TSPAN16   Affinity Capture-MS Homo sapiens 89 KIAA1804   Affinity Capture-MS Homo sapiens 90 ABCB6 10058 Affinity Capture-MS Homo sapiens 91 SMN1 6606 Two-hybrid Homo sapiens 92 TAMM41   Affinity Capture-MS Homo sapiens 93 SCGB1D1   Affinity Capture-MS Homo sapiens 94 COQ5   Affinity Capture-MS Homo sapiens 95 COQ6 51004 Affinity Capture-MS Homo sapiens 96 TMEM68   Affinity Capture-MS Homo sapiens 97 DSTYK 25778 Affinity Capture-MS Homo sapiens 98 VPS13B   Affinity Capture-MS Homo sapiens 99 MICU3   Affinity Capture-MS Homo sapiens 100 DOPEY2 9980 Affinity Capture-MS Homo sapiens 101 TIMM10   Affinity Capture-MS Homo sapiens 102 TUBB3 10381 Affinity Capture-MS Homo sapiens 103 EDEM2   Affinity Capture-MS Homo sapiens 104 UBE3C 9690 Affinity Capture-MS Homo sapiens 105 PRAME   Affinity Capture-MS Homo sapiens 106 MBNL1 4154 Affinity Capture-MS Homo sapiens 107 TBRG4 9238 Affinity Capture-MS Homo sapiens 108 KLK10 5655 Affinity Capture-MS Homo sapiens 109 NDC1 55706 Affinity Capture-MS Homo sapiens 110 GOT1 2805 Affinity Capture-MS Homo sapiens 111 HSDL1 83693 Affinity Capture-MS Homo sapiens 112 RHBDD1 84236 Affinity Capture-MS Homo sapiens 113 TAP1 6890 Affinity Capture-MS Homo sapiens 114 OR2A4 79541 Affinity Capture-MS Homo sapiens 115 ATP11C 286410 Affinity Capture-MS Homo sapiens 116 MATN2 4147 Affinity Capture-MS Homo sapiens 117 ALG11   Affinity Capture-MS Homo sapiens 118 P2RX6   Affinity Capture-MS Homo sapiens 119 PRKRIR   Affinity Capture-MS Homo sapiens View the network

Pathways in which IGHM is involved

No pathways found