Gene description for Ncbp1
Gene name nuclear cap binding protein subunit 1
Gene symbol Ncbp1
Other names/aliases AU014645
AW538051
CBP80
Species Mus musculus
 Database cross references - Ncbp1
ExoCarta ExoCarta_433702
Vesiclepedia VP_433702
Entrez Gene 433702
UniProt Q3UYV9  
 Ncbp1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ncbp1
Molecular Function
    RNA cap binding GO:0000339 IBA
    RNA 7-methylguanosine cap binding GO:0000340 IEA
    RNA 7-methylguanosine cap binding GO:0000340 ISO
    mRNA binding GO:0003729 IBA
    protein binding GO:0005515 IPI
    molecular adaptor activity GO:0060090 ISO
Biological Process
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 IBA
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 ISO
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 ISS
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 NAS
    spliceosomal complex assembly GO:0000245 IEA
    spliceosomal complex assembly GO:0000245 ISO
    alternative mRNA splicing, via spliceosome GO:0000380 NAS
    mRNA splicing, via spliceosome GO:0000398 NAS
    cap-dependent translational initiation GO:0002191 NAS
    7-methylguanosine mRNA capping GO:0006370 ISO
    7-methylguanosine mRNA capping GO:0006370 ISS
    RNA catabolic process GO:0006401 ISO
    mRNA export from nucleus GO:0006406 IBA
    mRNA export from nucleus GO:0006406 ISO
    mRNA export from nucleus GO:0006406 ISS
    snRNA export from nucleus GO:0006408 ISO
    snRNA export from nucleus GO:0006408 NAS
    regulation of translational initiation GO:0006446 ISO
    regulation of translational initiation GO:0006446 ISS
    histone mRNA metabolic process GO:0008334 IMP
    histone mRNA metabolic process GO:0008334 ISO
    mRNA metabolic process GO:0016071 ISO
    mRNA metabolic process GO:0016071 ISS
    positive regulation of cell growth GO:0030307 ISO
    positive regulation of cell growth GO:0030307 ISS
    primary miRNA processing GO:0031053 NAS
    mRNA 3'-end processing GO:0031124 NAS
    positive regulation of mRNA 3'-end processing GO:0031442 ISO
    positive regulation of mRNA 3'-end processing GO:0031442 ISS
    positive regulation of transcription elongation by RNA polymerase II GO:0032968 NAS
    regulatory ncRNA-mediated post-transcriptional gene silencing GO:0035194 NAS
    miRNA-mediated post-transcriptional gene silencing GO:0035195 NAS
    mRNA transcription by RNA polymerase II GO:0042789 ISO
    mRNA transcription by RNA polymerase II GO:0042789 NAS
    positive regulation of mRNA splicing, via spliceosome GO:0048026 IEA
    positive regulation of mRNA splicing, via spliceosome GO:0048026 ISO
    regulation of mRNA processing GO:0050684 IBA
    defense response to virus GO:0051607 ISO
    positive regulation of RNA binding GO:1905216 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 NAS
    mitochondrion GO:0005739 IEA
    mitochondrion GO:0005739 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    nuclear cap binding complex GO:0005846 IBA
    nuclear cap binding complex GO:0005846 ISO
    nuclear cap binding complex GO:0005846 ISS
    RNA cap binding complex GO:0034518 ISO
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Ncbp1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ncbp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Eed  
Affinity Capture-MS Mus musculus
2 Rpl13a 22121
Co-fractionation Mus musculus
3 Atxn2 20239
Co-fractionation Mus musculus
4 Stau1  
Co-fractionation Mus musculus
5 Ilf2 67781
Co-fractionation Mus musculus
6 Rps4x 20102
Co-fractionation Mus musculus
7 Ybx1 22608
Co-fractionation Mus musculus
8 Eif4e  
Co-fractionation Mus musculus
9 Hnrnpu 51810
Co-fractionation Mus musculus
10 Pus1  
Co-fractionation Mus musculus
11 Ncl 17975
Co-fractionation Mus musculus
12 Pabpn1 54196
Co-fractionation Mus musculus
13 Serpine2  
Co-fractionation Mus musculus
14 Ago2 239528
Co-fractionation Mus musculus
15 Hnrnpab 15384
Co-fractionation Mus musculus
16 Unk  
Affinity Capture-RNA Mus musculus
17 Poldip3  
Co-fractionation Mus musculus
18 Syncrip 56403
Co-fractionation Mus musculus
19 Rps3a1 20091
Co-fractionation Mus musculus
20 Rps9 76846
Co-fractionation Mus musculus
21 Rpl26 19941
Co-fractionation Mus musculus
22 Rplp0 11837
Co-fractionation Mus musculus
23 Ilf3 16201
Co-fractionation Mus musculus
24 Eprs 107508
Co-fractionation Mus musculus
25 Atg16l1  
Affinity Capture-MS Mus musculus
26 Hnrnpa2b1 53379
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Ncbp1 is involved
PathwayEvidenceSource
FGFR2 alternative splicing IEA Reactome
Formation of RNA Pol II elongation complex IEA Reactome
Formation of the Early Elongation Complex IEA Reactome
Gene expression (Transcription) IEA Reactome
Metabolism of RNA IEA Reactome
mRNA 3'-end processing IEA Reactome
mRNA Capping IEA Reactome
mRNA Splicing IEA Reactome
mRNA Splicing - Major Pathway IEA Reactome
mRNA Splicing - Minor Pathway IEA Reactome
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) IEA Reactome
Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) IEA Reactome
Nonsense-Mediated Decay (NMD) IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA IEA Reactome
Processing of Capped Intronless Pre-mRNA IEA Reactome
Processing of Intronless Pre-mRNAs IEA Reactome
RNA Polymerase II Pre-transcription Events IEA Reactome
RNA polymerase II transcribes snRNA genes IEA Reactome
RNA Polymerase II Transcription IEA Reactome
RNA Polymerase II Transcription Elongation IEA Reactome
RNA Polymerase II Transcription Termination IEA Reactome
Signal Transduction IEA Reactome
Signaling by FGFR IEA Reactome
Signaling by FGFR2 IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs IEA Reactome
SLBP independent Processing of Histone Pre-mRNAs IEA Reactome
Transport of Mature mRNA derived from an Intron-Containing Transcript IEA Reactome
Transport of Mature mRNA Derived from an Intronless Transcript IEA Reactome
Transport of Mature mRNAs Derived from Intronless Transcripts IEA Reactome
Transport of Mature Transcript to Cytoplasm IEA Reactome
Transport of the SLBP Dependant Mature mRNA IEA Reactome
Transport of the SLBP independent Mature mRNA IEA Reactome





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