Gene description for GREM2
Gene name gremlin 2, DAN family BMP antagonist
Gene symbol GREM2
Other names/aliases CKTSF1B2
DAND3
PRDC
Species Homo sapiens
 Database cross references - GREM2
ExoCarta ExoCarta_64388
Vesiclepedia VP_64388
Entrez Gene 64388
HGNC 17655
MIM 608832
UniProt Q9H772  
 GREM2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for GREM2
Molecular Function
    cytokine activity GO:0005125 IEA
    heparin binding GO:0008201 ISS
    BMP binding GO:0036122 IBA
    identical protein binding GO:0042802 IEA
    receptor ligand activity GO:0048018 IBA
Biological Process
    embryonic body morphogenesis GO:0010172 ISS
    cytokine-mediated signaling pathway GO:0019221 ISS
    sequestering of BMP from receptor via BMP binding GO:0038098 IBA
    determination of dorsal identity GO:0048263 IEA
    regulation of cytokine activity GO:0060300 ISS
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
 Experiment description of studies that identified GREM2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GREM2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZMYND19  
Affinity Capture-MS Homo sapiens
2 CST1  
Affinity Capture-MS Homo sapiens
3 SNX27 81609
Affinity Capture-MS Homo sapiens
4 RNF123 63891
Affinity Capture-MS Homo sapiens
5 FGFR1 2260
Affinity Capture-MS Homo sapiens
6 UBAC1 10422
Affinity Capture-MS Homo sapiens
7 FBN2 2201
Affinity Capture-MS Homo sapiens
8 SORL1 6653
Affinity Capture-MS Homo sapiens
9 NOTCH2 4853
Affinity Capture-MS Homo sapiens
10 HECTD3 79654
Affinity Capture-MS Homo sapiens
11 FERMT2 10979
Affinity Capture-MS Homo sapiens
12 ZZEF1 23140
Affinity Capture-MS Homo sapiens
13 SQSTM1 8878
Affinity Capture-MS Homo sapiens
14 DCAF16 54876
Affinity Capture-MS Homo sapiens
15 KBTBD7  
Affinity Capture-MS Homo sapiens
16 WAPAL  
Affinity Capture-MS Homo sapiens
17 PRKCI 5584
Affinity Capture-MS Homo sapiens
18 AHCYL2 23382
Affinity Capture-MS Homo sapiens
19 KBTBD6  
Affinity Capture-MS Homo sapiens
20 ANKRD52 283373
Affinity Capture-MS Homo sapiens
21 VWDE  
Affinity Capture-MS Homo sapiens
22 UBR1 197131
Affinity Capture-MS Homo sapiens
23 CST4 1472
Affinity Capture-MS Homo sapiens
24 HLA-F 3134
Affinity Capture-MS Homo sapiens
25 LRP2 4036
Affinity Capture-MS Homo sapiens
26 NACC1 112939
Affinity Capture-MS Homo sapiens
27 PPP2CA 5515
Affinity Capture-MS Homo sapiens
28 LDLR 3949
Affinity Capture-MS Homo sapiens
29 MAPK14 1432
Affinity Capture-MS Homo sapiens
30 BMP1 649
Affinity Capture-MS Homo sapiens
31 MKLN1  
Affinity Capture-MS Homo sapiens
32 WDHD1  
Affinity Capture-MS Homo sapiens
33 MAEA 10296
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GREM2 is involved
PathwayEvidenceSource
Signal Transduction TAS Reactome
Signaling by BMP TAS Reactome
Signaling by TGFB family members TAS Reactome





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