Gene description for Ndufa9
Gene name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9
Gene symbol Ndufa9
Other names/aliases 1010001N11Rik
Species Mus musculus
 Database cross references - Ndufa9
ExoCarta ExoCarta_66108
Vesiclepedia VP_66108
Entrez Gene 66108
UniProt Q9DC69  
 Ndufa9 identified in sEVs derived from the following tissue/cell type
Osteosarcoma cells 33785738    
Osteosarcoma cells 33785738    
Osteosarcoma cells 33785738    
 Gene ontology annotations for Ndufa9
Molecular Function
    NADH dehydrogenase activity GO:0003954 IEA
    NADH dehydrogenase activity GO:0003954 ISO
    protein binding GO:0005515 IPI
    protein-containing complex binding GO:0044877 IBA
    protein-containing complex binding GO:0044877 ISO
Biological Process
    ubiquinone biosynthetic process GO:0006744 IBA
    circadian rhythm GO:0007623 ISO
    circadian rhythm GO:0007623 ISS
    aerobic respiration GO:0009060 NAS
    mitochondrial respiratory chain complex I assembly GO:0032981 ISO
    mitochondrial respiratory chain complex I assembly GO:0032981 ISS
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 NAS
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 HDA
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrial matrix GO:0005759 IEA
    mitochondrial matrix GO:0005759 ISO
    mitochondrial membrane GO:0031966 ISO
    respiratory chain complex I GO:0045271 IDA
    respiratory chain complex I GO:0045271 ISO
 Experiment description of studies that identified Ndufa9 in sEVs
1
Experiment ID 1321
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33785738    
Organism Homo sapiens
Experiment description Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs
Authors "Todkar K, Chikhi L, Desjardins V, El-Mortada F, Pépin G, Germain M. "
Journal name Nat Commun
Publication year 2021
Sample Osteosarcoma cells
Sample name U2OS
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 1322
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33785738    
Organism Homo sapiens
Experiment description Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs
Authors "Todkar K, Chikhi L, Desjardins V, El-Mortada F, Pépin G, Germain M. "
Journal name Nat Commun
Publication year 2021
Sample Osteosarcoma cells
Sample name U2OS
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
3
Experiment ID 1323
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33785738    
Organism Homo sapiens
Experiment description Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs
Authors "Todkar K, Chikhi L, Desjardins V, El-Mortada F, Pépin G, Germain M. "
Journal name Nat Commun
Publication year 2021
Sample Osteosarcoma cells
Sample name U2OS
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
 Protein-protein interactions for Ndufa9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ndufa6  
Cross-Linking-MS (XL-MS) Mus musculus
2 Ndufs7  
Cross-Linking-MS (XL-MS) Mus musculus
3 Sdhb 67680
Cross-Linking-MS (XL-MS) Mus musculus
4 Ndufa9 66108
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
5 SNCA 6622
Affinity Capture-MS Homo sapiens
6 Atxn1  
Affinity Capture-MS Mus musculus
7 Cox5a  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
8 Kctd13  
Affinity Capture-MS Mus musculus
9 Ndufb5  
Co-fractionation Mus musculus
10 Htt  
Affinity Capture-MS Mus musculus
11 Dnaja3  
Co-fractionation Mus musculus
12 Slc25a4 11739
Cross-Linking-MS (XL-MS) Mus musculus
13 Sirt3  
Biochemical Activity Mus musculus
14 Hk2 15277
Cross-Linking-MS (XL-MS) Mus musculus
View the network image/svg+xml



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