Gene description for MED23
Gene name mediator complex subunit 23
Gene symbol MED23
Other names/aliases ARC130
CRSP130
CRSP133
CRSP3
DRIP130
MRT18
SUR-2
SUR2
Species Homo sapiens
 Database cross references - MED23
ExoCarta ExoCarta_9439
Vesiclepedia VP_9439
Entrez Gene 9439
HGNC 2372
MIM 605042
UniProt Q9ULK4  
 MED23 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Thymus 23844026    
 Gene ontology annotations for MED23
Molecular Function
    transcription coactivator activity GO:0003713 TAS
    protein binding GO:0005515 IPI
Biological Process
    regulation of DNA-templated transcription GO:0006355 IDA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    transcription initiation at RNA polymerase II promoter GO:0006367 TAS
    positive regulation of gene expression GO:0010628 IBA
    positive regulation of transcription elongation by RNA polymerase II GO:0032968 NAS
    RNA polymerase II preinitiation complex assembly GO:0051123 NAS
    positive regulation of transcription initiation by RNA polymerase II GO:0060261 NAS
    positive regulation of T cell extravasation GO:2000409 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    transcription regulator complex GO:0005667 IBA
    transcription regulator complex GO:0005667 IDA
    mediator complex GO:0016592 IBA
    core mediator complex GO:0070847 IPI
 Experiment description of studies that identified MED23 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MED23
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SGCA  
Affinity Capture-MS Homo sapiens
2 NCOA6  
Affinity Capture-Western Homo sapiens
3 MED17  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 MED7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 TRRAP 8295
Co-purification Homo sapiens
6 MED29  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 QKI 9444
Affinity Capture-MS Homo sapiens
8 FBXL19  
Affinity Capture-MS Homo sapiens
9 MED9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 MED13L 23389
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 C9orf78 51759
Affinity Capture-MS Homo sapiens
12 MED24 9862
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
13 ETS1  
Protein-peptide Homo sapiens
14 Med22  
Affinity Capture-MS Mus musculus
15 MED12L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 MED13 9969
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 CEBPB  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
18 HLA-B 3106
Affinity Capture-MS Homo sapiens
19 HARS2 23438
Co-fractionation Homo sapiens
20 FBXW7  
Affinity Capture-MS Homo sapiens
21 MED27  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ABCE1 6059
Affinity Capture-MS Homo sapiens
23 MED22  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
24 BRD4 23476
Affinity Capture-MS Homo sapiens
25 MECP2 4204
Affinity Capture-MS Homo sapiens
26 VDR  
Affinity Capture-MS Homo sapiens
27 GPR17 2840
Affinity Capture-MS Homo sapiens
28 MED4 29079
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 MED1 5469
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 MED20 9477
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 CTDP1 9150
Reconstituted Complex Homo sapiens
32 TRIM37  
Proximity Label-MS Homo sapiens
33 NTRK1 4914
Affinity Capture-MS Homo sapiens
34 GPR182  
Affinity Capture-MS Homo sapiens
35 OPA1 4976
Co-fractionation Homo sapiens
36 BCL6  
Affinity Capture-MS Homo sapiens
37 PML 5371
Affinity Capture-MS Homo sapiens
38 MED25  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
39 HECTD1 25831
Affinity Capture-MS Homo sapiens
40 MED31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 SCN3B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 CEBPA  
Protein-peptide Homo sapiens
43 NR1I3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 MED15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 KDF1  
Affinity Capture-MS Homo sapiens
46 PDXP 57026
Co-fractionation Homo sapiens
47 SLX4  
Affinity Capture-MS Homo sapiens
48 COPS2 9318
Affinity Capture-MS Homo sapiens
49 CCNC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 EPAS1  
Affinity Capture-MS Homo sapiens
51 SREBF1 6720
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
52 MED26  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 CDK19 23097
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 OBFC1 79991
Affinity Capture-MS Homo sapiens
55 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
56 HNF4A  
Affinity Capture-Western Homo sapiens
57 PSMB10 5699
Affinity Capture-MS Homo sapiens
58 MED10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
59 TADA2A  
Affinity Capture-MS Homo sapiens
60 RPA3 6119
Proximity Label-MS Homo sapiens
61 EHMT2 10919
Affinity Capture-Western Homo sapiens
62 NIPBL 25836
Affinity Capture-Western Homo sapiens
63 Rpl35 66489
Affinity Capture-MS Mus musculus
64 BRD3 8019
Affinity Capture-MS Homo sapiens
65 MED14  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
66 HIF1A 3091
Affinity Capture-Western Homo sapiens
67 MED11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 DZIP3  
Affinity Capture-MS Homo sapiens
69 RELA 5970
Reconstituted Complex Homo sapiens
70 PSMC3IP  
Affinity Capture-MS Homo sapiens
71 MED19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 GLUD1 2746
Co-fractionation Homo sapiens
73 ESR2  
Reconstituted Complex Homo sapiens
74 SIRT6  
Affinity Capture-MS Homo sapiens
75 PARP1 142
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
76 CDK8 1024
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 MED8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 MED6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
80 ESR1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
81 MED30  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
82 ELF3 1999
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Phenotypic Suppression Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
83 MED16 10025
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 MED18  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 MED21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 TCL1B  
Affinity Capture-MS Homo sapiens
87 MED12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 TRIP4 9325
Affinity Capture-MS Homo sapiens
89 RXRA 6256
Co-fractionation Homo sapiens
90 XRCC6 2547
Co-fractionation Homo sapiens
91 ZC3H13 23091
Affinity Capture-MS Homo sapiens
92 HSPA12A 259217
Co-fractionation Homo sapiens
93 NOTCH1 4851
Affinity Capture-MS Homo sapiens
94 MED28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MED23 is involved
PathwayEvidenceSource
Adipogenesis TAS Reactome
Adipogenesis IEA Reactome
Developmental Biology TAS Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
Epigenetic regulation by WDR5-containing histone modifying complexes TAS Reactome
Epigenetic regulation by WDR5-containing histone modifying complexes IEA Reactome
Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes TAS Reactome
Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes IEA Reactome
Epigenetic regulation of gene expression TAS Reactome
Epigenetic regulation of gene expression IEA Reactome
Epigenetic regulation of gene expression by MLL3 and MLL4 complexes TAS Reactome
Epigenetic regulation of gene expression by MLL3 and MLL4 complexes IEA Reactome
Gene expression (Transcription) TAS Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway TAS Reactome
Infectious disease TAS Reactome
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis TAS Reactome
MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis IEA Reactome
PPARA activates gene expression TAS Reactome
Regulation of lipid metabolism by PPARalpha TAS Reactome
Respiratory Syncytial Virus Infection Pathway TAS Reactome
RNA Polymerase II Transcription TAS Reactome
RSV-host interactions TAS Reactome
Transcriptional regulation of white adipocyte differentiation TAS Reactome
Transcriptional regulation of white adipocyte differentiation IEA Reactome
Viral Infection Pathways TAS Reactome





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