Gene description for MED13
Gene name mediator complex subunit 13
Gene symbol MED13
Other names/aliases ARC250
DRIP250
HSPC221
THRAP1
TRAP240
Species Homo sapiens
 Database cross references - MED13
ExoCarta ExoCarta_9969
Vesiclepedia VP_9969
Entrez Gene 9969
HGNC 22474
MIM 603808
UniProt Q9UHV7  
 MED13 identified in sEVs derived from the following tissue/cell type
Endothelial cells 26027894    
Hepatocytes 26054723    
 Gene ontology annotations for MED13
Molecular Function
    transcription coregulator activity GO:0003712 IDA
    transcription coactivator activity GO:0003713 IBA
    transcription coactivator activity GO:0003713 IDA
    protein binding GO:0005515 IPI
    nuclear receptor coactivator activity GO:0030374 NAS
    nuclear vitamin D receptor binding GO:0042809 NAS
    nuclear thyroid hormone receptor binding GO:0046966 IDA
Biological Process
    cholesterol homeostasis GO:0042632 IEA
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IBA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription initiation by RNA polymerase II GO:0060261 IDA
    triglyceride homeostasis GO:0070328 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    membrane GO:0016020 HDA
    mediator complex GO:0016592 IBA
    mediator complex GO:0016592 IDA
    CKM complex GO:1990508 IPI
    CKM complex GO:1990508 ISS
 Experiment description of studies that identified MED13 in sEVs
1
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for MED13
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MED11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 POLR2B 5431
Affinity Capture-MS Homo sapiens
3 POLR2C 5432
Affinity Capture-MS Homo sapiens
4 RPAP2  
Affinity Capture-MS Homo sapiens
5 C12orf49  
Positive Genetic Homo sapiens
6 MED19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
7 VDR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 POLR2G 5436
Affinity Capture-MS Homo sapiens
9 MED8  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
10 POLR2H 5437
Affinity Capture-MS Homo sapiens
11 MED17  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 TFRC 7037
Cross-Linking-MS (XL-MS) Homo sapiens
13 MED20 9477
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 CCNC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CDK8 1024
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 TRRAP 8295
Co-purification Homo sapiens
17 MED4 29079
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
18 MED1 5469
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
19 RB1 5925
Negative Genetic Homo sapiens
20 MED29  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
21 POLR2E 5434
Affinity Capture-MS Homo sapiens
22 QKI 9444
Affinity Capture-MS Homo sapiens
23 SREBF1 6720
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
24 FBXL19  
Affinity Capture-MS Homo sapiens
25 MED9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 EFTUD2 9343
Co-fractionation Homo sapiens
27 MED13L 23389
Affinity Capture-MS Homo sapiens
28 CDK19 23097
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 MED7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PRPF8 10594
Co-fractionation Homo sapiens
31 MED6  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
32 MED12L  
Affinity Capture-MS Homo sapiens
33 OBFC1 79991
Affinity Capture-MS Homo sapiens
34 FASN 2194
Positive Genetic Homo sapiens
35 POLR2A 5430
Affinity Capture-MS Homo sapiens
36 ACAD11 84129
Affinity Capture-MS Homo sapiens
37 PTRH1  
Affinity Capture-MS Homo sapiens
38 ESR1  
Reconstituted Complex Homo sapiens
39 MED30  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
40 MED16 10025
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
41 Med22  
Affinity Capture-MS Mus musculus
42 TCL1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 MED18  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
44 MED21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
45 MED10  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
46 FBXW7  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 MED12  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 TADA2A  
Affinity Capture-MS Homo sapiens
49 MED24 9862
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
50 TRIP4 9325
Affinity Capture-MS Homo sapiens
51 MED26  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 RANBP2 5903
Co-fractionation Homo sapiens
53 POLR2I 5438
Affinity Capture-MS Homo sapiens
54 SUPT3H  
Affinity Capture-Western Homo sapiens
55 MED23 9439
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 TSC22D2 9819
Two-hybrid Homo sapiens
57 MED25  
Affinity Capture-MS Homo sapiens
58 ZC3H13 23091
Affinity Capture-MS Homo sapiens
59 MED27  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
60 MED31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 KEAP1 9817
Negative Genetic Homo sapiens
62 MED15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 POLR1D 51082
Affinity Capture-MS Homo sapiens
64 MED14  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
65 MED22  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 NR1I3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 AR 367
Proximity Label-MS Homo sapiens
68 MED28  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 HIF1A 3091
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which MED13 is involved
PathwayEvidenceSource
Adipogenesis TAS Reactome
Adipogenesis IEA Reactome
Developmental Biology TAS Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
Epigenetic regulation by WDR5-containing histone modifying complexes TAS Reactome
Epigenetic regulation by WDR5-containing histone modifying complexes IEA Reactome
Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes TAS Reactome
Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes IEA Reactome
Epigenetic regulation of gene expression TAS Reactome
Epigenetic regulation of gene expression IEA Reactome
Epigenetic regulation of gene expression by MLL3 and MLL4 complexes TAS Reactome
Epigenetic regulation of gene expression by MLL3 and MLL4 complexes IEA Reactome
Gene expression (Transcription) TAS Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway TAS Reactome
Infectious disease TAS Reactome
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis TAS Reactome
MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis IEA Reactome
PPARA activates gene expression TAS Reactome
Regulation of lipid metabolism by PPARalpha TAS Reactome
Respiratory Syncytial Virus Infection Pathway TAS Reactome
RNA Polymerase II Transcription TAS Reactome
RSV-host interactions TAS Reactome
Transcriptional regulation of white adipocyte differentiation TAS Reactome
Transcriptional regulation of white adipocyte differentiation IEA Reactome
Viral Infection Pathways TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here