Gene description for ROCK2
Gene name Rho-associated, coiled-coil containing protein kinase 2
Gene symbol ROCK2
Other names/aliases ROCK-II
Species Homo sapiens
 Database cross references - ROCK2
ExoCarta ExoCarta_9475
Vesiclepedia VP_9475
Entrez Gene 9475
HGNC 10252
MIM 604002
UniProt O75116  
 ROCK2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 17956143    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for ROCK2
Molecular Function
    protease binding GO:0002020 IPI
    RNA binding GO:0003723 HDA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 IMP
    protein serine/threonine kinase activity GO:0004674 ISS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    structural molecule activity GO:0005198 NAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    small GTPase binding GO:0031267 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    metal ion binding GO:0046872 IEA
    tau protein binding GO:0048156 NAS
    tau-protein kinase activity GO:0050321 NAS
    endopeptidase activator activity GO:0061133 IMP
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IBA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IMP
    Rho-dependent protein serine/threonine kinase activity GO:0072518 TAS
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    mitotic cytokinesis GO:0000281 IBA
    epithelial to mesenchymal transition GO:0001837 ISS
    positive regulation of protein phosphorylation GO:0001934 IMP
    response to ischemia GO:0002931 ISS
    aortic valve morphogenesis GO:0003180 ISS
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    smooth muscle contraction GO:0006939 TAS
    Rho protein signal transduction GO:0007266 IBA
    Rho protein signal transduction GO:0007266 IDA
    positive regulation of endothelial cell migration GO:0010595 IMP
    positive regulation of cardiac muscle hypertrophy GO:0010613 ISS
    positive regulation of gene expression GO:0010628 IMP
    positive regulation of gene expression GO:0010628 ISS
    negative regulation of gene expression GO:0010629 ISS
    positive regulation of centrosome duplication GO:0010825 IEA
    negative regulation of angiogenesis GO:0016525 IMP
    actin cytoskeleton organization GO:0030036 ISS
    regulation of cell adhesion GO:0030155 TAS
    positive regulation of cell migration GO:0030335 IMP
    cortical actin cytoskeleton organization GO:0030866 IBA
    actomyosin structure organization GO:0031032 IBA
    regulation of nervous system process GO:0031644 ISS
    positive regulation of connective tissue growth factor production GO:0032723 ISS
    regulation of actin cytoskeleton organization GO:0032956 IBA
    regulation of actin cytoskeleton organization GO:0032956 TAS
    regulation of circadian rhythm GO:0042752 ISS
    positive regulation of MAPK cascade GO:0043410 ISS
    modulation by host of viral process GO:0044788 IMP
    negative regulation of nitric oxide biosynthetic process GO:0045019 IMP
    negative regulation of nitric oxide biosynthetic process GO:0045019 ISS
    regulation of keratinocyte differentiation GO:0045616 IMP
    rhythmic process GO:0048511 IEA
    embryonic morphogenesis GO:0048598 IBA
    centrosome duplication GO:0051298 IMP
    regulation of stress fiber assembly GO:0051492 TAS
    positive regulation of stress fiber assembly GO:0051496 IDA
    positive regulation of stress fiber assembly GO:0051496 IMP
    regulation of focal adhesion assembly GO:0051893 TAS
    mRNA destabilization GO:0061157 ISS
    negative regulation of biomineral tissue development GO:0070168 ISS
    cellular response to testosterone stimulus GO:0071394 IMP
    response to transforming growth factor beta GO:0071559 ISS
    protein localization to plasma membrane GO:0072659 IGI
    protein localization to plasma membrane GO:0072659 IMP
    positive regulation of fibroblast growth factor production GO:0090271 ISS
    blood vessel diameter maintenance GO:0097746 ISS
    regulation of angiotensin-activated signaling pathway GO:0110061 ISS
    negative regulation of protein localization to lysosome GO:0150033 IMP
    regulation of cellular response to hypoxia GO:1900037 ISS
    regulation of cell junction assembly GO:1901888 IBA
    positive regulation of amyloid-beta formation GO:1902004 IMP
    positive regulation of protein localization to early endosome GO:1902966 IMP
    positive regulation of amyloid precursor protein catabolic process GO:1902993 IMP
    regulation of establishment of endothelial barrier GO:1903140 IGI
    regulation of establishment of endothelial barrier GO:1903140 IMP
    negative regulation of bicellular tight junction assembly GO:1903347 IGI
    cellular response to acetylcholine GO:1905145 ISS
    positive regulation of connective tissue replacement GO:1905205 ISS
    response to angiotensin GO:1990776 ISS
    regulation of establishment of cell polarity GO:2000114 TAS
    regulation of cell motility GO:2000145 TAS
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IBA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IBA
    plasma membrane GO:0005886 IEA
    cytoplasmic ribonucleoprotein granule GO:0036464 IDA
 Experiment description of studies that identified ROCK2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
10
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
17
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
18
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
20
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
22
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ROCK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTPRF 5792
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
2 SPRTN  
Affinity Capture-MS Homo sapiens
3 MED17  
Affinity Capture-MS Homo sapiens
4 DNAJC8 22826
Affinity Capture-MS Homo sapiens
5 LAMP3  
Proximity Label-MS Homo sapiens
6 GJA1 2697
Proximity Label-MS Homo sapiens
7 RACGAP1 29127
Co-fractionation Homo sapiens
8 RPA2 6118
Proximity Label-MS Homo sapiens
9 RPL10 6134
Affinity Capture-MS Homo sapiens
10 LGALS1 3956
Affinity Capture-MS Homo sapiens
11 EFTUD1 79631
Co-fractionation Homo sapiens
12 FAM21A 387680
Co-fractionation Homo sapiens
13 LAMP2 3920
Proximity Label-MS Homo sapiens
14 MILR1  
Affinity Capture-MS Homo sapiens
15 FBXO42  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 APEX1 328
Affinity Capture-RNA Homo sapiens
17 KIAA1429 25962
Affinity Capture-MS Homo sapiens
18 RPS6KA3 6197
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 GPHN 10243
Co-fractionation Homo sapiens
20 TLN1 7094
Co-fractionation Homo sapiens
21 ANKRD49  
Affinity Capture-MS Homo sapiens
22 ADD1 118
Co-fractionation Homo sapiens
23 TDG 6996
Co-fractionation Homo sapiens
24 RHOA 387
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 PLCG1 5335
Co-fractionation Homo sapiens
26 IKBKE 9641
Affinity Capture-MS Homo sapiens
27 PFN2 5217
Co-fractionation Homo sapiens
28 SEC23A 10484
Co-fractionation Homo sapiens
29 CYLD  
Affinity Capture-MS Homo sapiens
30 PLCB3 5331
Co-fractionation Homo sapiens
31 GOLGA8G  
Affinity Capture-MS Homo sapiens
32 MTA2 9219
Co-fractionation Homo sapiens
33 LAMTOR1 55004
Proximity Label-MS Homo sapiens
34 KIAA0196 9897
Co-fractionation Homo sapiens
35 HSPA8 3312
Co-fractionation Homo sapiens
36 LZTFL1 54585
Affinity Capture-MS Homo sapiens
37 RABGEF1 27342
Co-fractionation Homo sapiens
38 NSUN2 54888
Co-fractionation Homo sapiens
39 ZNF444  
Affinity Capture-MS Homo sapiens
40 PRPH 5630
Proximity Label-MS Homo sapiens
41 Rock1  
Affinity Capture-MS Mus musculus
42 MCAM 4162
Proximity Label-MS Homo sapiens
43 MAGEC1  
Two-hybrid Homo sapiens
44 UBE2I 7329
Biochemical Activity Homo sapiens
45 ADD2 119
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 CDH1 999
Co-fractionation Homo sapiens
47 BBS1 582
Affinity Capture-MS Homo sapiens
48 NTRK1 4914
Affinity Capture-MS Homo sapiens
49 CDKN3 1033
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
50 ACTB 60
Co-fractionation Homo sapiens
51 RYBP  
Affinity Capture-MS Homo sapiens
52 DNAJB11 51726
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
53 ZBTB2 57621
Affinity Capture-MS Homo sapiens
54 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
55 NCKAP1 10787
Co-fractionation Homo sapiens
56 LAMP1 3916
Proximity Label-MS Homo sapiens
57 CUL3 8452
Affinity Capture-MS Homo sapiens
58 TYW3  
Affinity Capture-MS Homo sapiens
59 EGFL7 51162
Affinity Capture-MS Homo sapiens
60 ARF6 382
Proximity Label-MS Homo sapiens
61 DUSP16  
Affinity Capture-MS Homo sapiens
62 CORO1C 23603
Co-fractionation Homo sapiens
63 HTRA3 94031
Affinity Capture-MS Homo sapiens
64 SEC24A 10802
Co-fractionation Homo sapiens
65 MAPRE3  
Proximity Label-MS Homo sapiens
66 ZW10 9183
Co-fractionation Homo sapiens
67 PIAS1 8554
Affinity Capture-Western Homo sapiens
68 RPA3 6119
Proximity Label-MS Homo sapiens
69 FBXO2 26232
Affinity Capture-MS Homo sapiens
70 AHR 196
Affinity Capture-MS Homo sapiens
71 SUV39H2 79723
Affinity Capture-MS Homo sapiens
72 RHOBTB1  
Affinity Capture-Western Homo sapiens
73 NHP2L1 4809
Co-fractionation Homo sapiens
74 NDC80 10403
Co-fractionation Homo sapiens
75 DNAJC3 5611
Co-fractionation Homo sapiens
76 RHOB 388
Proximity Label-MS Homo sapiens
77 TXNDC5 81567
Co-fractionation Homo sapiens
78 LMNA 4000
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
79 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
80 SNX9 51429
Cross-Linking-MS (XL-MS) Homo sapiens
81 STK16 8576
Affinity Capture-MS Homo sapiens
82 ROCK1 6093
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
83 TBC1D9  
Affinity Capture-MS Homo sapiens
84 POLR1C 9533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 SLC39A7 7922
Affinity Capture-MS Homo sapiens
86 PFDN1 5201
Affinity Capture-MS Homo sapiens
87 SMAD4  
Affinity Capture-MS Homo sapiens
88 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
89 MYL2  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
90 PHKA2 5256
Affinity Capture-MS Homo sapiens
91 LRPAP1 4043
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 RAB2A 5862
Proximity Label-MS Homo sapiens
93 SPPL2B 56928
Affinity Capture-MS Homo sapiens
94 TRIP4 9325
Affinity Capture-MS Homo sapiens
95 RAB11A 8766
Proximity Label-MS Homo sapiens
96 EPHA2 1969
Proximity Label-MS Homo sapiens
97 ZNF641  
Affinity Capture-MS Homo sapiens
98 SNW1 22938
Affinity Capture-MS Homo sapiens
99 KRAS 3845
Proximity Label-MS Homo sapiens
100 CYFIP1 23191
Co-fractionation Homo sapiens
101 DFFA 1676
Co-fractionation Homo sapiens
102 STRBP 55342
Co-fractionation Homo sapiens
103 LURAP1  
Affinity Capture-MS Homo sapiens
104 EP300 2033
Affinity Capture-MS Homo sapiens
105 CDC5L 988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ROCK2 is involved
PathwayEvidenceSource
Axon guidance IEA Reactome
Axon guidance TAS Reactome
Developmental Biology IEA Reactome
Developmental Biology TAS Reactome
Disease TAS Reactome
EPH-Ephrin signaling IEA Reactome
EPH-Ephrin signaling TAS Reactome
EPHA-mediated growth cone collapse IEA Reactome
EPHB-mediated forward signaling IEA Reactome
EPHB-mediated forward signaling TAS Reactome
G alpha (12/13) signalling events TAS Reactome
GPCR downstream signalling TAS Reactome
Infectious disease TAS Reactome
Nervous system development IEA Reactome
Nervous system development TAS Reactome
Potential therapeutics for SARS TAS Reactome
RHO GTPase cycle TAS Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPases Activate ROCKs IEA Reactome
RHO GTPases Activate ROCKs TAS Reactome
RHOA GTPase cycle TAS Reactome
RHOB GTPase cycle TAS Reactome
RHOBTB GTPase Cycle TAS Reactome
RHOBTB1 GTPase cycle TAS Reactome
RHOC GTPase cycle TAS Reactome
RHOH GTPase cycle TAS Reactome
SARS-CoV Infections TAS Reactome
Sema4D in semaphorin signaling IEA Reactome
Sema4D in semaphorin signaling TAS Reactome
Sema4D induced cell migration and growth-cone collapse IEA Reactome
Sema4D induced cell migration and growth-cone collapse TAS Reactome
Semaphorin interactions IEA Reactome
Semaphorin interactions TAS Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by GPCR TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by VEGF TAS Reactome
VEGFA-VEGFR2 Pathway TAS Reactome
Viral Infection Pathways TAS Reactome





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