Gene description for CD36
Gene name CD36 molecule (thrombospondin receptor)
Gene symbol CD36
Other names/aliases BDPLT10
CHDS7
FAT
GP3B
GP4
GPIV
PASIV
SCARB3
Species Homo sapiens
 Database cross references - CD36
ExoCarta ExoCarta_948
Vesiclepedia VP_948
Entrez Gene 948
HGNC 1663
MIM 173510
UniProt P16671  
 CD36 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast milk 17641064    
Breast milk 17641064    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Urine 20224111    
Urine 20224111    
 Gene ontology annotations for CD36
Molecular Function
    amyloid-beta binding GO:0001540 IC
    amyloid-beta binding GO:0001540 IDA
    amyloid-beta binding GO:0001540 TAS
    low-density lipoprotein particle receptor activity GO:0005041 IBA
    low-density lipoprotein particle receptor activity GO:0005041 IMP
    low-density lipoprotein particle receptor activity GO:0005041 TAS
    scavenger receptor activity GO:0005044 IBA
    scavenger receptor activity GO:0005044 IMP
    scavenger receptor activity GO:0005044 TAS
    long-chain fatty acid transmembrane transporter activity GO:0005324 IMP
    long-chain fatty acid transmembrane transporter activity GO:0005324 TAS
    protein binding GO:0005515 IPI
    high-density lipoprotein particle binding GO:0008035 IEA
    lipid binding GO:0008289 IDA
    short-chain fatty acid transmembrane transporter activity GO:0015636 IMP
    low-density lipoprotein particle binding GO:0030169 IBA
    low-density lipoprotein particle binding GO:0030169 IDA
    Toll-like receptor binding GO:0035325 IPI
    cargo receptor activity GO:0038024 IDA
    protein-containing complex binding GO:0044877 IPI
    transforming growth factor beta binding GO:0050431 ISS
    thrombospondin receptor activity GO:0070053 IDA
    thrombospondin receptor activity GO:0070053 ISS
    lipoteichoic acid immune receptor activity GO:0070892 IEA
    lipoprotein particle binding GO:0071813 IDA
    oxidised low-density lipoprotein particle receptor activity GO:0150025 IMP
    oleate transmembrane transporter activity GO:1901480 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    MAPK cascade GO:0000165 IEA
    positive regulation of cell-matrix adhesion GO:0001954 IDA
    production of molecular mediator involved in inflammatory response GO:0002532 TAS
    lipid metabolic process GO:0006629 NAS
    fatty acid metabolic process GO:0006631 TAS
    receptor-mediated endocytosis GO:0006898 IBA
    receptor-mediated endocytosis GO:0006898 IMP
    phagocytosis, recognition GO:0006910 IEA
    phagocytosis, engulfment GO:0006911 TAS
    cell adhesion GO:0007155 IEA
    cell surface receptor signaling pathway GO:0007166 IEA
    positive regulation of cytosolic calcium ion concentration GO:0007204 ISS
    blood coagulation GO:0007596 TAS
    positive regulation of gene expression GO:0010628 ISS
    negative regulation of gene expression GO:0010629 IEA
    positive regulation of macrophage derived foam cell differentiation GO:0010744 IMP
    positive regulation of cholesterol storage GO:0010886 IEA
    fatty acid transport GO:0015908 IDA
    long-chain fatty acid transport GO:0015909 IMP
    long-chain fatty acid import across plasma membrane GO:0015911 IDA
    short-chain fatty acid transport GO:0015912 IMP
    negative regulation of angiogenesis GO:0016525 IDA
    lipid storage GO:0019915 IBA
    lipid storage GO:0019915 IMP
    cGMP-mediated signaling GO:0019934 IDA
    positive regulation of blood coagulation GO:0030194 IEA
    intestinal cholesterol absorption GO:0030299 ISS
    cholesterol transport GO:0030301 ISS
    receptor internalization GO:0031623 ISS
    regulation of lipopolysaccharide-mediated signaling pathway GO:0031664 TAS
    positive regulation of interleukin-1 beta production GO:0032731 ISS
    positive regulation of interleukin-12 production GO:0032735 IEA
    positive regulation of interleukin-6 production GO:0032755 IEA
    positive regulation of tumor necrosis factor production GO:0032760 IEA
    response to lipid GO:0033993 ISS
    regulation of toll-like receptor signaling pathway GO:0034121 IGI
    triglyceride transport GO:0034197 ISS
    plasma lipoprotein particle clearance GO:0034381 ISS
    low-density lipoprotein particle clearance GO:0034383 IBA
    low-density lipoprotein particle clearance GO:0034383 IMP
    response to stilbenoid GO:0035634 IEA
    nitric oxide-cGMP-mediated signaling GO:0038060 IDA
    negative regulation of protein import into nucleus GO:0042308 IEA
    lipoprotein transport GO:0042953 IBA
    lipoprotein transport GO:0042953 IMP
    lipoprotein transport GO:0042953 TAS
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IGI
    regulation of protein-containing complex assembly GO:0043254 IMP
    apoptotic cell clearance GO:0043277 IEA
    long-chain fatty acid import into cell GO:0044539 IBA
    long-chain fatty acid import into cell GO:0044539 IDA
    positive regulation of nitric oxide biosynthetic process GO:0045429 ISS
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IEA
    defense response to Gram-positive bacterium GO:0050830 IEA
    intestinal absorption GO:0050892 ISS
    sensory perception of taste GO:0050909 ISS
    low-density lipoprotein particle mediated signaling GO:0055096 IEA
    positive regulation of phagocytosis, engulfment GO:0060100 IEA
    positive regulation of macrophage cytokine production GO:0060907 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISS
    cholesterol import GO:0070508 ISS
    response to fatty acid GO:0070542 ISS
    response to linoleic acid GO:0070543 ISS
    cellular response to lipopolysaccharide GO:0071222 IEA
    cellular response to lipoteichoic acid GO:0071223 IEA
    cellular response to low-density lipoprotein particle stimulus GO:0071404 ISS
    cellular response to hydroperoxide GO:0071447 IEA
    cellular response to diacyl bacterial lipopeptide GO:0071726 IDA
    cellular response to diacyl bacterial lipopeptide GO:0071726 ISS
    energy homeostasis GO:0097009 ISS
    regulation of action potential GO:0098900 ISS
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
    cellular response to oxidised low-density lipoprotein particle stimulus GO:0140052 IMP
    oxidised low-density lipoprotein particle clearance GO:0150024 IMP
    amyloid-beta clearance by cellular catabolic process GO:0150094 IBA
    amyloid-beta clearance by cellular catabolic process GO:0150094 IMP
    positive regulation of NLRP3 inflammasome complex assembly GO:1900227 ISS
    positive regulation of reactive oxygen species biosynthetic process GO:1903428 ISS
    cellular response to amyloid-beta GO:1904646 IGI
    amyloid fibril formation GO:1990000 ISS
    lipid transport across blood-brain barrier GO:1990379 IMP
    regulation of removal of superoxide radicals GO:2000121 IEA
    positive regulation of blood microparticle formation GO:2000334 IEA
Subcellular Localization
    extracellular space GO:0005615 HDA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    caveola GO:0005901 IBA
    external side of plasma membrane GO:0009897 IEA
    cell surface GO:0009986 IBA
    cell surface GO:0009986 IDA
    cell surface GO:0009986 ISS
    cell surface GO:0009986 TAS
    membrane GO:0016020 TAS
    apical plasma membrane GO:0016324 IEA
    endocytic vesicle membrane GO:0030666 TAS
    platelet alpha granule membrane GO:0031092 TAS
    brush border membrane GO:0031526 ISS
    specific granule membrane GO:0035579 TAS
    receptor complex GO:0043235 IPI
    membrane raft GO:0045121 IDA
    phagocytic vesicle GO:0045335 TAS
    cell periphery GO:0071944 IDA
 Experiment description of studies that identified CD36 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 46
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSC70|CD63|MHCII|CD81|CD86
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Colostrum
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
6
Experiment ID 48
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSC70|CD63|CD86|MHCII
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Mature milk
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
7
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 77
MISEV standards
✘ Biophysical techniques
✔
CD81|CD9|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
11
Experiment ID 78
MISEV standards
✘ Biophysical techniques
✔
CD81|CD9|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Urine
Sample name Urine - Bladder cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
 Protein-protein interactions for CD36
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ITGB1 3688
Affinity Capture-Western Homo sapiens
2 MBOAT7 79143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 LY6H  
Reconstituted Complex Homo sapiens
4 VLDLR 7436
Reconstituted Complex Homo sapiens
5 ITGA2B 3674
Affinity Capture-Western Homo sapiens
6 GPC4 2239
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
7 MATK  
Affinity Capture-Western Homo sapiens
8 HSD11B1 3290
Reconstituted Complex Homo sapiens
9 ITGB3 3690
Affinity Capture-Western Homo sapiens
10 USP10 9100
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 CD9 928
Affinity Capture-Western Homo sapiens
12 ITGA6 3655
Affinity Capture-Western Homo sapiens
13 CAND1 55832
Affinity Capture-MS Homo sapiens
14 APP 351
Reconstituted Complex Homo sapiens
15 LYN 4067
Affinity Capture-Western Homo sapiens
16 SCRG1  
Reconstituted Complex Homo sapiens
17 CROT 54677
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 FYN 2534
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
19 CHRD  
Two-hybrid Homo sapiens
20 YES1 7525
Affinity Capture-Western Homo sapiens
21 LDLR 3949
Reconstituted Complex Homo sapiens
22 NRN1  
Reconstituted Complex Homo sapiens
23 C3orf52  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 VWC2L  
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which CD36 is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
Adipogenesis TAS Reactome
Antigen processing-Cross presentation TAS Reactome
Binding and Uptake of Ligands by Scavenger Receptors IEA Reactome
Binding and Uptake of Ligands by Scavenger Receptors TAS Reactome
Class I MHC mediated antigen processing & presentation TAS Reactome
Cross-presentation of particulate exogenous antigens (phagosomes) TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Developmental Biology TAS Reactome
Disease TAS Reactome
Diseases associated with the TLR signaling cascade TAS Reactome
Diseases of Immune System TAS Reactome
Epigenetic regulation by WDR5-containing histone modifying complexes IEA Reactome
Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes IEA Reactome
Epigenetic regulation of gene expression IEA Reactome
Epigenetic regulation of gene expression by MLL3 and MLL4 complexes IEA Reactome
ER-Phagosome pathway TAS Reactome
Free fatty acids regulate insulin secretion TAS Reactome
Gene expression (Transcription) IEA Reactome
Hemostasis TAS Reactome
Immune System TAS Reactome
Innate Immune System TAS Reactome
Integration of energy metabolism TAS Reactome
Interleukin-4 and Interleukin-13 signaling TAS Reactome
Intracellular metabolism of fatty acids regulates insulin secretion TAS Reactome
IRAK4 deficiency (TLR2/4) TAS Reactome
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis IEA Reactome
MyD88 deficiency (TLR2/4) TAS Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane TAS Reactome
Neutrophil degranulation TAS Reactome
Platelet activation, signaling and aggregation TAS Reactome
Platelet degranulation TAS Reactome
PPARA activates gene expression TAS Reactome
Regulation of insulin secretion TAS Reactome
Regulation of lipid metabolism by PPARalpha TAS Reactome
Regulation of TLR by endogenous ligand TAS Reactome
Response to elevated platelet cytosolic Ca2+ TAS Reactome
Scavenging by Class B Receptors IEA Reactome
Scavenging by Class B Receptors TAS Reactome
Signaling by Interleukins TAS Reactome
Toll Like Receptor 2 (TLR2) Cascade TAS Reactome
Toll Like Receptor 4 (TLR4) Cascade TAS Reactome
Toll Like Receptor TLR1:TLR2 Cascade TAS Reactome
Toll Like Receptor TLR6:TLR2 Cascade TAS Reactome
Toll-like Receptor Cascades TAS Reactome
Transcriptional regulation of white adipocyte differentiation TAS Reactome
Vesicle-mediated transport IEA Reactome
Vesicle-mediated transport TAS Reactome





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