Gene description for C2CD5
Gene name C2 calcium-dependent domain containing 5
Gene symbol C2CD5
Other names/aliases CDP138
KIAA0528
Species Homo sapiens
 Database cross references - C2CD5
ExoCarta ExoCarta_9847
Vesiclepedia VP_9847
Entrez Gene 9847
HGNC 29062
UniProt Q86YS7  
 C2CD5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for C2CD5
Molecular Function
    calcium ion binding GO:0005509 IBA
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    calcium-dependent phospholipid binding GO:0005544 IBA
    calcium-dependent phospholipid binding GO:0005544 IDA
Biological Process
    insulin receptor signaling pathway GO:0008286 IDA
    insulin receptor signaling pathway GO:0008286 ISS
    positive regulation of D-glucose transmembrane transport GO:0010828 IBA
    positive regulation of D-glucose transmembrane transport GO:0010828 ISS
    positive regulation of vesicle fusion GO:0031340 IBA
    positive regulation of vesicle fusion GO:0031340 IMP
    intracellular protein transmembrane transport GO:0065002 IBA
    intracellular protein transmembrane transport GO:0065002 IMP
    protein localization to plasma membrane GO:0072659 IBA
    positive regulation of protein targeting to membrane GO:0090314 IBA
    positive regulation of protein targeting to membrane GO:0090314 IMP
Subcellular Localization
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    cell cortex GO:0005938 IDA
    cytoplasmic vesicle membrane GO:0030659 IDA
    ruffle membrane GO:0032587 IDA
    centriolar satellite GO:0034451 IDA
 Experiment description of studies that identified C2CD5 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for C2CD5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNAJC7 7266
Proximity Label-MS Homo sapiens
2 TACR3  
Affinity Capture-MS Homo sapiens
3 Pcdh1  
Affinity Capture-MS Mus musculus
4 NAGK 55577
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 LYN 4067
Proximity Label-MS Homo sapiens
6 HNRNPD 3184
Affinity Capture-MS Homo sapiens
7 LGALS9 3965
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 LYPD3 27076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ESRRA  
Co-fractionation Homo sapiens
10 FASN 2194
Negative Genetic Homo sapiens
11 SIRT6  
Affinity Capture-MS Homo sapiens
12 TNFRSF1B  
Affinity Capture-MS Homo sapiens
13 MCAM 4162
Proximity Label-MS Homo sapiens
14 WDFY2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 RBM8A 9939
Affinity Capture-MS Homo sapiens
16 KIF14 9928
Affinity Capture-MS Homo sapiens
17 ZCCHC10  
Affinity Capture-MS Homo sapiens
18 RACGAP1 29127
Affinity Capture-MS Homo sapiens
19 DNAJA2 10294
Proximity Label-MS Homo sapiens
20 FGF12  
Affinity Capture-MS Homo sapiens
21 IL13 3596
Affinity Capture-MS Homo sapiens
22 RAB35 11021
Proximity Label-MS Homo sapiens
23 BRD2  
Affinity Capture-MS Homo sapiens
24 MIB1 57534
Proximity Label-MS Homo sapiens
25 LZTS2 84445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 Smek1  
Affinity Capture-MS Mus musculus
27 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
28 SLC5A6 8884
Affinity Capture-MS Homo sapiens
29 DLK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 C11orf52 91894
Proximity Label-MS Homo sapiens
31 PTGER3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 BRD3 8019
Affinity Capture-MS Homo sapiens
33 SASS6 163786
Affinity Capture-MS Homo sapiens
34 Ect2  
Affinity Capture-MS Mus musculus
35 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
36 BAG6 7917
Affinity Capture-MS Homo sapiens
37 ITGB3BP  
Affinity Capture-MS Homo sapiens
38 SGTB  
Affinity Capture-MS Homo sapiens
39 CDK2 1017
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 QSOX2 169714
Affinity Capture-MS Homo sapiens
41 CDK5 1020
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 NTRK1 4914
Affinity Capture-MS Homo sapiens
43 SORT1 6272
Affinity Capture-MS Homo sapiens
44 Lck 16818
Affinity Capture-MS Mus musculus
45 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
46 EP300 2033
Affinity Capture-MS Homo sapiens
47 TCTEX1D4  
Affinity Capture-MS Homo sapiens
48 DLL1  
Affinity Capture-MS Homo sapiens
49 EPHA2 1969
Proximity Label-MS Homo sapiens
50 ZUFSP 221302
Affinity Capture-MS Homo sapiens
51 ARRDC3 57561
Affinity Capture-MS Homo sapiens
52 Naa10  
Affinity Capture-MS Mus musculus
53 KRAS 3845
Proximity Label-MS Homo sapiens
54 RAB5C 5878
Affinity Capture-MS Homo sapiens
55 RHOB 388
Proximity Label-MS Homo sapiens
56 RXRB 6257
Affinity Capture-MS Homo sapiens
57 PPP6R1 22870
Proximity Label-MS Homo sapiens
58 SPANXN2  
Affinity Capture-MS Homo sapiens
59 TMIE  
Affinity Capture-MS Homo sapiens
60 SYT2  
Affinity Capture-MS Homo sapiens
61 CCDC102B  
Affinity Capture-MS Homo sapiens
62 AIFM2 84883
Affinity Capture-MS Homo sapiens
63 AVPR1B  
Affinity Capture-MS Homo sapiens
64 FIBP 9158
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 BRD4 23476
Affinity Capture-MS Homo sapiens
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