Gene description for MET
Gene name MET proto-oncogene, receptor tyrosine kinase
Gene symbol MET
Other names/aliases AUTS9
DFNB97
HGFR
RCCP2
c-Met
Species Homo sapiens
 Database cross references - MET
ExoCarta ExoCarta_4233
Vesiclepedia VP_4233
Entrez Gene 4233
HGNC 7029
MIM 164860
UniProt P08581  
 MET identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 34887515    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for MET
Molecular Function
    protein tyrosine kinase activity GO:0004713 NAS
    protein tyrosine kinase activity GO:0004713 TAS
    GPI-linked ephrin receptor activity GO:0005004 IEA
    transmembrane-ephrin receptor activity GO:0005005 IEA
    epidermal growth factor receptor activity GO:0005006 IEA
    fibroblast growth factor receptor activity GO:0005007 IEA
    hepatocyte growth factor receptor activity GO:0005008 IBA
    insulin receptor activity GO:0005009 IEA
    insulin-like growth factor receptor activity GO:0005010 IEA
    macrophage colony-stimulating factor receptor activity GO:0005011 IEA
    platelet-derived growth factor alpha-receptor activity GO:0005018 IEA
    platelet-derived growth factor beta-receptor activity GO:0005019 IEA
    stem cell factor receptor activity GO:0005020 IEA
    vascular endothelial growth factor receptor activity GO:0005021 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    boss receptor activity GO:0008288 IEA
    semaphorin receptor activity GO:0017154 IEA
    protein phosphatase binding GO:0019903 IPI
    histone H3Y41 kinase activity GO:0035401 IEA
    placental growth factor receptor activity GO:0036332 IEA
    protein tyrosine kinase collagen receptor activity GO:0038062 IEA
    identical protein binding GO:0042802 IPI
    brain-derived neurotrophic factor receptor activity GO:0060175 IEA
    molecular function activator activity GO:0140677 EXP
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    endothelial cell morphogenesis GO:0001886 IDA
    liver development GO:0001889 IBA
    chromatin remodeling GO:0006338 IEA
    signal transduction GO:0007165 TAS
    cell surface receptor signaling pathway GO:0007166 NAS
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    epidermal growth factor receptor signaling pathway GO:0007173 IEA
    multicellular organism development GO:0007275 IBA
    insulin receptor signaling pathway GO:0008286 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 IEA
    negative regulation of autophagy GO:0010507 NAS
    neuron differentiation GO:0030182 IBA
    pancreas development GO:0031016 IBA
    positive regulation of microtubule polymerization GO:0031116 IMP
    brain-derived neurotrophic factor receptor signaling pathway GO:0031547 IEA
    negative regulation of Rho protein signal transduction GO:0035024 IDA
    platelet-derived growth factor receptor-alpha signaling pathway GO:0035790 IEA
    platelet-derived growth factor receptor-beta signaling pathway GO:0035791 IEA
    vascular endothelial growth factor receptor-1 signaling pathway GO:0036323 IEA
    collagen-activated tyrosine kinase receptor signaling pathway GO:0038063 IEA
    vascular endothelial growth factor signaling pathway GO:0038084 IEA
    Kit signaling pathway GO:0038109 IEA
    macrophage colony-stimulating factor signaling pathway GO:0038145 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    insulin-like growth factor receptor signaling pathway GO:0048009 IEA
    hepatocyte growth factor receptor signaling pathway GO:0048012 IEA
    ephrin receptor signaling pathway GO:0048013 IEA
    branching morphogenesis of an epithelial tube GO:0048754 IMP
    positive chemotaxis GO:0050918 IDA
    negative regulation of stress fiber assembly GO:0051497 IDA
    excitatory postsynaptic potential GO:0060079 ISS
    establishment of skin barrier GO:0061436 IMP
    negative regulation of thrombin-activated receptor signaling pathway GO:0070495 IDA
    semaphorin-plexin signaling pathway GO:0071526 IDA
    negative regulation of hydrogen peroxide-mediated programmed cell death GO:1901299 IMP
    negative regulation of guanyl-nucleotide exchange factor activity GO:1905098 IDA
    positive regulation of endothelial cell chemotaxis GO:2001028 IMP
Subcellular Localization
    extracellular region GO:0005576 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    basal plasma membrane GO:0009925 IBA
    basal plasma membrane GO:0009925 IDA
    cell surface GO:0009986 HDA
    membrane GO:0016020 NAS
    receptor complex GO:0043235 IBA
    postsynapse GO:0098794 IEA
 Experiment description of studies that identified MET in sEVs
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
4
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
5
Experiment ID 1207
MISEV standards
✘ Biophysical techniques
✔
SDCBP|CD9|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name HCA-7 SC
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
6
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
10
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
11
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
16
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
18
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MET
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TNS1 7145
Reconstituted Complex Homo sapiens
2 ABL2  
Reconstituted Complex Homo sapiens
3 SHC1 6464
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 MATK  
Reconstituted Complex Homo sapiens
5 CASP3 836
Co-localization Homo sapiens
6 GJA1 2697
Proximity Label-MS Homo sapiens
7 CRK 1398
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 SPSB4  
Affinity Capture-Western Homo sapiens
9 LGALS1 3956
Affinity Capture-MS Homo sapiens
10 SOCS1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 HSP90AB1 3326
Affinity Capture-Western Homo sapiens
12 VHL  
Negative Genetic Homo sapiens
13 SLURP1  
Affinity Capture-MS Homo sapiens
14 PTPN11 5781
Reconstituted Complex Homo sapiens
15 GRB7 2886
Reconstituted Complex Homo sapiens
16 HSPA4 3308
Affinity Capture-Western Homo sapiens
17 ARL6IP6  
Affinity Capture-MS Homo sapiens
18 GNG12 55970
Co-fractionation Homo sapiens
19 PLCG2 5336
Reconstituted Complex Homo sapiens
20 HCK 3055
Reconstituted Complex Homo sapiens
21 RANBP10 57610
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
22 ZAP70 7535
Reconstituted Complex Homo sapiens
23 FES 2242
Reconstituted Complex Homo sapiens
24 YES1 7525
Reconstituted Complex Homo sapiens
25 HGS 9146
Affinity Capture-Western Homo sapiens
26 SRC 6714
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
27 STAT3 6774
Affinity Capture-Western Homo sapiens
28 SYK 6850
Reconstituted Complex Homo sapiens
29 ST14 6768
Affinity Capture-MS Homo sapiens
30 C11orf52 91894
Proximity Label-MS Homo sapiens
31 SOCS5  
Reconstituted Complex Homo sapiens
32 PLCG1 5335
Reconstituted Complex Homo sapiens
33 SHC4  
Reconstituted Complex Homo sapiens
34 SFN 2810
Affinity Capture-Western Homo sapiens
35 TNS4 84951
Reconstituted Complex Homo sapiens
36 LGALS9 3965
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 SOCS3 9021
Reconstituted Complex Homo sapiens
38 BTNL2  
Affinity Capture-MS Homo sapiens
39 NCK1 4690
Reconstituted Complex Homo sapiens
40 BCAR3 8412
Reconstituted Complex Homo sapiens
41 CLGN 1047
Affinity Capture-MS Homo sapiens
42 NRSN1  
Affinity Capture-MS Homo sapiens
43 SOCS6  
Reconstituted Complex Homo sapiens
44 CEACAM21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 ERBB3 2065
Affinity Capture-Western Homo sapiens
46 SPSB2  
Affinity Capture-Western Homo sapiens
47 LYN 4067
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
48 CBL 867
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
49 SPSB3  
Affinity Capture-Western Homo sapiens
50 TEC 7006
Reconstituted Complex Homo sapiens
51 PTK6  
Reconstituted Complex Homo sapiens
52 DNAJC5 80331
Proximity Label-MS Homo sapiens
53 SH2B2  
Reconstituted Complex Homo sapiens
54 CDH1 999
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
55 SH3BP2  
Reconstituted Complex Homo sapiens
56 SH2D1A 4068
Reconstituted Complex Homo sapiens
57 C1orf54  
Affinity Capture-MS Homo sapiens
58 CHEK1  
Negative Genetic Homo sapiens
59 HGF 3082
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
60 ITK 3702
Reconstituted Complex Homo sapiens
61 BTK 695
Reconstituted Complex Homo sapiens
62 VAV2 7410
Reconstituted Complex Homo sapiens
63 PTPRO 5800
Affinity Capture-MS Homo sapiens
64 STAP1  
Reconstituted Complex Homo sapiens
65 MET 4233
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
66 LY86  
Affinity Capture-MS Homo sapiens
67 TXK  
Reconstituted Complex Homo sapiens
68 GPIHBP1  
Affinity Capture-MS Homo sapiens
69 FGD5  
Affinity Capture-Western Homo sapiens
70 CCL22 6367
Affinity Capture-MS Homo sapiens
71 SPSB1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
72 SOCS2  
Reconstituted Complex Homo sapiens
73 MARCH1  
Affinity Capture-MS Homo sapiens
74 GLMN 11146
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
75 SH2D1B  
Reconstituted Complex Homo sapiens
76 CTNNB1 1499
Affinity Capture-Western Homo sapiens
77 SHB 6461
Reconstituted Complex Homo sapiens
78 PTEN 5728
Positive Genetic Homo sapiens
79 GGA3 23163
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
80 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
81 FBXO6 26270
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 TNS3 64759
Reconstituted Complex Homo sapiens
83 GAB1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
84 DCN 1634
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
85 BLK 640
Reconstituted Complex Homo sapiens
86 RANBP9 10048
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
87 PCBP1 5093
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 LRIG1 26018
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
89 FBXO2 26232
Affinity Capture-MS Homo sapiens
90 SH2B1 25970
Reconstituted Complex Homo sapiens
91 TNS2 23371
Reconstituted Complex Homo sapiens
92 ERBB2 2064
PCA Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
93 GRB14 2888
Reconstituted Complex Homo sapiens
94 SHC3  
Reconstituted Complex Homo sapiens
95 HSH2D 84941
Reconstituted Complex Homo sapiens
96 PIK3R1 5295
Reconstituted Complex Homo sapiens
97 CBLC  
Affinity Capture-Western Homo sapiens
98 STUB1 10273
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
99 RASA1 5921
Reconstituted Complex Homo sapiens
100 CRLF2 64109
Affinity Capture-MS Homo sapiens
101 FGR 2268
Reconstituted Complex Homo sapiens
102 SLA2 84174
Reconstituted Complex Homo sapiens
103 SHD  
Reconstituted Complex Homo sapiens
104 GRB2 2885
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
105 SCGB1D1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 INPPL1 3636
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
107 ASIC4  
Affinity Capture-MS Homo sapiens
108 SH2D3C 10044
Reconstituted Complex Homo sapiens
109 SH2B3  
Reconstituted Complex Homo sapiens
110 RYK 6259
Affinity Capture-MS Homo sapiens
111 LCK 3932
Reconstituted Complex Homo sapiens
112 SOS1 6654
Affinity Capture-Western Homo sapiens
113 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
114 GAB2 9846
Affinity Capture-Western Homo sapiens
115 SH2D2A  
Reconstituted Complex Homo sapiens
116 NCK2 8440
Reconstituted Complex Homo sapiens
117 SFTPC  
Affinity Capture-MS Homo sapiens
118 BRICD5  
Affinity Capture-MS Homo sapiens
119 EPHA2 1969
Proximity Label-MS Homo sapiens
120 PIK3R2 5296
Reconstituted Complex Homo sapiens
121 KRAS 3845
Proximity Label-MS Homo sapiens
122 SH3KBP1 30011
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
123 VAV3 10451
Reconstituted Complex Homo sapiens
124 CAV1 857
Affinity Capture-Western Homo sapiens
125 PIK3R3 8503
Reconstituted Complex Homo sapiens
126 SHC2 25759
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which MET is involved
PathwayEvidenceSource
Axon guidance TAS Reactome
Bacterial Infection Pathways TAS Reactome
Bacterial Infection Pathways IEA Reactome
Constitutive Signaling by Aberrant PI3K in Cancer TAS Reactome
Developmental Biology TAS Reactome
Disease TAS Reactome
Disease IEA Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Drug-mediated inhibition of MET activation TAS Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
InlB-mediated entry of Listeria monocytogenes into host cell TAS Reactome
InlB-mediated entry of Listeria monocytogenes into host cell IEA Reactome
Intracellular signaling by second messengers TAS Reactome
Listeria monocytogenes entry into host cells TAS Reactome
Listeria monocytogenes entry into host cells IEA Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
MECP2 regulates neuronal receptors and channels TAS Reactome
MET activates PI3K/AKT signaling TAS Reactome
MET activates PTK2 signaling TAS Reactome
MET activates PTPN11 TAS Reactome
MET activates RAP1 and RAC1 TAS Reactome
MET activates RAS signaling TAS Reactome
MET activates STAT3 TAS Reactome
MET interacts with TNS proteins TAS Reactome
MET promotes cell motility TAS Reactome
MET Receptor Activation TAS Reactome
MET receptor recycling TAS Reactome
MITF-M-dependent gene expression TAS Reactome
MITF-M-regulated melanocyte development TAS Reactome
Negative regulation of MET activity TAS Reactome
Negative regulation of the PI3K/AKT network TAS Reactome
Nervous system development TAS Reactome
PI3K/AKT Signaling in Cancer TAS Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling TAS Reactome
PIP3 activates AKT signaling TAS Reactome
RAF/MAP kinase cascade TAS Reactome
Regulation of MITF-M-dependent genes involved in cell cycle and proliferation TAS Reactome
RNA Polymerase II Transcription TAS Reactome
Sema4D in semaphorin signaling TAS Reactome
Sema4D mediated inhibition of cell attachment and migration TAS Reactome
Semaphorin interactions TAS Reactome
Signal Transduction TAS Reactome
Signaling by MET TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Transcriptional Regulation by MECP2 TAS Reactome





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