Experiment description of studies that identified MISP in sEVs

1 Experiment ID 207 MISEV standards ✔ Biophysical techniques ✔ Enriched markers ✔ Negative markers ✔ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 208 MISEV standards ✔ Biophysical techniques ✔ Enriched markers ✔ Negative markers ✔ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 209 MISEV standards ✘ Biophysical techniques ✔ Enriched markers ✔ Negative markers ✔ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 211 MISEV standards ✔ Biophysical techniques ✔ Enriched markers ✔ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 212 MISEV standards ✔ Biophysical techniques ✔ Enriched markers ✔ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for MISP

Protein Interactor ExoCarta ID Identification method PubMed Species 1 HNRNPH1 3187 Affinity Capture-RNA Homo sapiens 2 ACTR2 10097 Affinity Capture-MS Homo sapiens 3 ISG15 9636 Affinity Capture-MS Homo sapiens 4 Cdk1 12534 Affinity Capture-MS Mus musculus 5 PPP1CB 5500 Affinity Capture-MS Homo sapiens 6 KLK15   Affinity Capture-MS Homo sapiens 7 Tpm1 22003 Affinity Capture-MS Mus musculus 8 CENPE 1062 Affinity Capture-MS Homo sapiens 9 FSTL4   Affinity Capture-MS Homo sapiens 10 Calml3   Affinity Capture-MS Mus musculus 11 GAK 2580 Affinity Capture-MS Homo sapiens 12 KIF20A 10112 Affinity Capture-MS Homo sapiens 13 MYO5C 55930 Affinity Capture-MS Homo sapiens 14 B3GALT4   Affinity Capture-MS Homo sapiens 15 Actb 11461 Affinity Capture-MS Mus musculus 16 EPB41L1 2036 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 17 RPS6KA3 6197 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 18 RDX 5962 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 19 Trim69   Affinity Capture-MS Mus musculus 20 KIF23 9493 Affinity Capture-MS Homo sapiens 21 D2HGDH 728294 Affinity Capture-MS Homo sapiens 22 Poc1b   Affinity Capture-MS Mus musculus 23 PRC1 9055 Affinity Capture-MS Homo sapiens 24 IQGAP1 8826 Affinity Capture-MS Homo sapiens 25 GPR55   Affinity Capture-MS Homo sapiens 26 OBSL1 23363 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 PRELP 5549 Affinity Capture-MS Homo sapiens 28 Tubgcp3   Affinity Capture-MS Mus musculus 29 Flot2 14252 Affinity Capture-MS Mus musculus 30 Spast   Affinity Capture-MS Mus musculus 31 AMD1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 32 DCTN1 1639 Affinity Capture-Western Homo sapiens 33 DNAJC7 7266 Affinity Capture-MS Homo sapiens 34 RPS6KA2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 35 CD48 962 Affinity Capture-MS Homo sapiens 36 NPAS1   Affinity Capture-MS Homo sapiens 37 Erh   Affinity Capture-MS Mus musculus 38 Lima1   Affinity Capture-MS Mus musculus 39 KIF14 9928 Affinity Capture-MS Homo sapiens 40 DYRK1B   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 41 C1orf106   Affinity Capture-MS Homo sapiens 42 CHMP4C 92421 Affinity Capture-MS Homo sapiens 43 EFNA2   Affinity Capture-MS Homo sapiens 44 FAM96A   Affinity Capture-MS Homo sapiens 45 MYO18A 399687 Affinity Capture-MS Homo sapiens 46 SULT1C4   Affinity Capture-MS Homo sapiens 47 HIST1H4A 8359 Affinity Capture-MS Homo sapiens 48 B4GALT2   Affinity Capture-MS Homo sapiens 49 MAPRE1 22919 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 50 RNF4 6047 Affinity Capture-MS Homo sapiens 51 RCCD1   Affinity Capture-MS Homo sapiens 52 CHMP4B 128866 Affinity Capture-MS Homo sapiens 53 MYH9 4627 Affinity Capture-MS Homo sapiens 54 Stag2   Affinity Capture-MS Mus musculus 55 FANCD2   Affinity Capture-MS Homo sapiens 56 CLEC11A 6320 Affinity Capture-MS Homo sapiens 57 ADAMTS1 9510 Affinity Capture-MS Homo sapiens 58 ARL4C 10123 Affinity Capture-MS Homo sapiens 59 DBN1 1627 Affinity Capture-MS Homo sapiens 60 UBXN6 80700 Affinity Capture-MS Homo sapiens 61 RPS6KA1 6195 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 62 KLHL14 57565 Affinity Capture-MS Homo sapiens 63 ANLN 54443 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 64 PHLPP1   Proximity Label-MS Homo sapiens 65 LIMA1 51474 Affinity Capture-MS Homo sapiens 66 HLA-DPB1 3115 Affinity Capture-MS Homo sapiens 67 BRF1   Affinity Capture-MS Homo sapiens 68 TUBB 203068 Affinity Capture-MS Homo sapiens 69 Myh9 17886 Affinity Capture-MS Mus musculus 70 NUBP2 10101 Affinity Capture-MS Homo sapiens 71 ECT2 1894 Affinity Capture-MS Homo sapiens 72 ERLEC1 27248 Affinity Capture-MS Homo sapiens 73 MYO19   Affinity Capture-MS Homo sapiens 74 FARP2 9855 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 75 PTK2 5747 Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 76 MYO1C 4641 Affinity Capture-MS Homo sapiens 77 EPHA7 2045 Affinity Capture-MS Homo sapiens 78 SYNPO 11346 Affinity Capture-MS Homo sapiens 79 Flnb 286940 Affinity Capture-MS Mus musculus 80 IL12RB1   Affinity Capture-MS Homo sapiens 81 MSN 4478 Affinity Capture-MS Homo sapiens 82 FSCN1 6624 Affinity Capture-MS Homo sapiens 83 Ppp1cb 19046 Affinity Capture-MS Mus musculus 84 Coro1c 23790 Affinity Capture-MS Mus musculus 85 PDLIM7 9260 Affinity Capture-MS Homo sapiens 86 RABEPK   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 87 NAAA   Affinity Capture-MS Homo sapiens 88 FOXRED2   Affinity Capture-MS Homo sapiens 89 L3HYPDH 112849 Affinity Capture-MS Homo sapiens 90 HSPA12A 259217 Affinity Capture-MS Homo sapiens 91 KLHDC3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 92 EZR 7430 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 93 Myo1c 17913 Affinity Capture-MS Mus musculus 94 CAPZA2 830 Affinity Capture-MS Homo sapiens 95 ZDHHC23   Affinity Capture-MS Homo sapiens 96 Sass6   Affinity Capture-MS Mus musculus 97 CDK1 983 Biochemical Activity Homo sapiens 98 Myh10 77579 Affinity Capture-MS Mus musculus 99 KLHL1   Affinity Capture-MS Homo sapiens 100 EPB41L3 23136 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 101 ADAMTS12 81792 Affinity Capture-MS Homo sapiens 102 VWA2 340706 Affinity Capture-MS Homo sapiens 103 FLNA 2316 Affinity Capture-MS Homo sapiens 104 PINK1   Affinity Capture-MS Homo sapiens 105 Flot1 14251 Affinity Capture-MS Mus musculus 106 ANXA2 302 Affinity Capture-MS Homo sapiens 107 CAPN10   Affinity Capture-MS Homo sapiens 108 MEN1 4221 Affinity Capture-MS Homo sapiens 109 Sept9   Affinity Capture-MS Mus musculus 110 Mau2   Affinity Capture-MS Mus musculus View the network

Pathways in which MISP is involved

No pathways found