Gene description for Flnb
Gene name filamin, beta
Gene symbol Flnb
Other names/aliases AL024016
Fln-b
Species Mus musculus
 Database cross references - Flnb
ExoCarta ExoCarta_286940
Vesiclepedia VP_286940
Entrez Gene 286940
UniProt Q80X90  
 Flnb identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Flnb
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    actin filament binding GO:0051015 IEA
Biological Process
    keratinocyte development GO:0003334 IMP
    epithelial cell morphogenesis GO:0003382 IMP
    skeletal muscle tissue development GO:0007519 IDA
    actin cytoskeleton organization GO:0030036 IMP
    cellular response to type II interferon GO:0071346 IDA
Subcellular Localization
    stress fiber GO:0001725 IDA
    cytoplasm GO:0005737 ISA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    brush border GO:0005903 IDA
    focal adhesion GO:0005925 IDA
    cell cortex GO:0005938 IEA
    Z disc GO:0030018 IEA
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
    phagocytic vesicle GO:0045335 IDA
 Experiment description of studies that identified Flnb in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Flnb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARHGAP21 57584
Affinity Capture-MS Homo sapiens
2 AP2B1 163
Affinity Capture-MS Homo sapiens
3 DECR2 26063
Affinity Capture-MS Homo sapiens
4 ZDHHC5 25921
Affinity Capture-MS Homo sapiens
5 RAB1A 5861
Affinity Capture-MS Homo sapiens
6 SNAP23 8773
Affinity Capture-MS Homo sapiens
7 ARPC5L 81873
Affinity Capture-MS Homo sapiens
8 CPM 1368
Affinity Capture-MS Homo sapiens
9 G3BP1 10146
Affinity Capture-MS Homo sapiens
10 DBN1 1627
Affinity Capture-MS Homo sapiens
11 EPS15 2060
Affinity Capture-MS Homo sapiens
12 LGALS1 3956
Affinity Capture-MS Homo sapiens
13 CALM1 801
Affinity Capture-MS Homo sapiens
14 CLTB 1212
Affinity Capture-MS Homo sapiens
15 PI4KA 5297
Affinity Capture-MS Homo sapiens
16 PPP1CC 5501
Affinity Capture-MS Homo sapiens
17 PPP1R18 170954
Affinity Capture-MS Homo sapiens
18 CAPZB 832
Affinity Capture-MS Homo sapiens
19 AFAP1 60312
Affinity Capture-MS Homo sapiens
20 ABLIM1 3983
Affinity Capture-MS Homo sapiens
21 AP2A1 160
Affinity Capture-MS Homo sapiens
22 CD109 135228
Affinity Capture-MS Homo sapiens
23 YES1 7525
Affinity Capture-MS Homo sapiens
24 DSC2 1824
Affinity Capture-MS Homo sapiens
25 RHOA 387
Affinity Capture-MS Homo sapiens
26 RHOB 388
Affinity Capture-MS Homo sapiens
27 MYO1B 4430
Affinity Capture-MS Homo sapiens
28 TPRN 286262
Affinity Capture-MS Homo sapiens
29 Eed  
Affinity Capture-MS Mus musculus
30 PALM 5064
Affinity Capture-MS Homo sapiens
31 SYNPO 11346
Affinity Capture-MS Homo sapiens
32 CYBRD1 79901
Affinity Capture-MS Homo sapiens
33 COBL  
Affinity Capture-MS Homo sapiens
34 CAPZA1 829
Affinity Capture-MS Homo sapiens
35 RAB14 51552
Affinity Capture-MS Homo sapiens
36 SORBS2  
Affinity Capture-MS Homo sapiens
37 LUZP1 7798
Affinity Capture-MS Homo sapiens
38 RAI14 26064
Affinity Capture-MS Homo sapiens
39 RALA 5898
Affinity Capture-MS Homo sapiens
40 TMOD1  
Affinity Capture-MS Homo sapiens
41 LYN 4067
Affinity Capture-MS Homo sapiens
42 RRAS2 22800
Affinity Capture-MS Homo sapiens
43 RAP1B 5908
Affinity Capture-MS Homo sapiens
44 FYN 2534
Affinity Capture-MS Homo sapiens
45 NUMB 8650
Affinity Capture-MS Homo sapiens
46 ACTN4 81
Affinity Capture-MS Homo sapiens
47 LIN7C 55327
Affinity Capture-MS Homo sapiens
48 SPRYD3 84926
Affinity Capture-MS Homo sapiens
49 RAP1A 5906
Affinity Capture-MS Homo sapiens
50 HACE1 57531
Affinity Capture-MS Homo sapiens
51 BMP2K 55589
Affinity Capture-MS Homo sapiens
52 ARPC5 10092
Affinity Capture-MS Homo sapiens
53 FLOT2 2319
Affinity Capture-MS Homo sapiens
54 ACTG1 71
Affinity Capture-MS Homo sapiens
55 RAP2C 57826
Affinity Capture-MS Homo sapiens
56 Ubc  
Reconstituted Complex Mus musculus
57 LIMA1 51474
Affinity Capture-MS Homo sapiens
58 FLNA 2316
Affinity Capture-MS Homo sapiens
59 RALB 5899
Affinity Capture-MS Homo sapiens
60 AHNAK 79026
Affinity Capture-MS Homo sapiens
61 PRKAR2A 5576
Affinity Capture-MS Homo sapiens
62 CTTN 2017
Affinity Capture-MS Homo sapiens
63 SPECC1 92521
Affinity Capture-MS Homo sapiens
64 MYO5A 4644
Affinity Capture-MS Homo sapiens
65 PACSIN3 29763
Affinity Capture-MS Homo sapiens
66 CD59 966
Affinity Capture-MS Homo sapiens
67 BASP1 10409
Affinity Capture-MS Homo sapiens
68 MYO1C 4641
Affinity Capture-MS Homo sapiens
69 AP2M1 1173
Affinity Capture-MS Homo sapiens
70 Nphp4  
Affinity Capture-MS Mus musculus
71 RRAS 6237
Affinity Capture-MS Homo sapiens
72 TMEM237 65062
Affinity Capture-MS Homo sapiens
73 FCHO2 115548
Affinity Capture-MS Homo sapiens
74 Pou5f1  
Affinity Capture-MS Mus musculus
75 RPL22 6146
Affinity Capture-MS Homo sapiens
76 PPFIBP1 8496
Affinity Capture-MS Homo sapiens
77 Rnf123  
Affinity Capture-MS Mus musculus
78 AKAP2 11217
Affinity Capture-MS Homo sapiens
79 CLTC 1213
Affinity Capture-MS Homo sapiens
80 KIDINS220 57498
Affinity Capture-MS Homo sapiens
81 FLOT1 10211
Affinity Capture-MS Homo sapiens
82 FARP1 10160
Affinity Capture-MS Homo sapiens
83 Fancd2  
Affinity Capture-MS Mus musculus
84 SVIL 6840
Affinity Capture-MS Homo sapiens
85 SPTBN1 6711
Affinity Capture-MS Homo sapiens
86 NEXN 91624
Affinity Capture-MS Homo sapiens
87 SPTAN1 6709
Affinity Capture-MS Homo sapiens
88 DAB2 1601
Affinity Capture-MS Homo sapiens
89 JUP 3728
Affinity Capture-MS Homo sapiens
90 PLEC 5339
Affinity Capture-MS Homo sapiens
91 MISP 126353
Affinity Capture-MS Homo sapiens
92 Atxn1  
Affinity Capture-MS Mus musculus
93 Kctd13  
Affinity Capture-MS Mus musculus
94 CAPZA2 830
Affinity Capture-MS Homo sapiens
95 CFL2 1073
Affinity Capture-MS Homo sapiens
96 Iqcb1  
Affinity Capture-MS Mus musculus
97 Mettl21e  
Affinity Capture-MS Mus musculus
98 INF2 64423
Affinity Capture-MS Homo sapiens
99 CORO2A  
Affinity Capture-MS Homo sapiens
100 DAPK3 1613
Affinity Capture-MS Homo sapiens
101 RAB7A 7879
Affinity Capture-MS Homo sapiens
102 MYO5C 55930
Affinity Capture-MS Homo sapiens
103 KRAS 3845
Affinity Capture-MS Homo sapiens
104 RAB5C 5878
Affinity Capture-MS Homo sapiens
105 CD44 960
Affinity Capture-MS Homo sapiens
106 RAC1 5879
Affinity Capture-MS Homo sapiens
107 ANXA2 302
Affinity Capture-MS Homo sapiens
108 SNTB2 6645
Affinity Capture-MS Homo sapiens
109 DSG2 1829
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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