Gene description for NUMB
Gene name numb homolog (Drosophila)
Gene symbol NUMB
Other names/aliases C14orf41
S171
c14_5527
Species Homo sapiens
 Database cross references - NUMB
ExoCarta ExoCarta_8650
Vesiclepedia VP_8650
Entrez Gene 8650
HGNC 8060
MIM 603728
UniProt P49757  
 NUMB identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for NUMB
Molecular Function
    protein binding GO:0005515 IPI
    beta-catenin binding GO:0008013 IEA
    alpha-catenin binding GO:0045294 IEA
    cadherin binding GO:0045296 HDA
Biological Process
    axonogenesis GO:0007409 IEA
    lateral ventricle development GO:0021670 ISS
    neuroblast division in subventricular zone GO:0021849 ISS
    positive regulation of cell migration GO:0030335 IGI
    positive regulation of cell migration GO:0030335 IMP
    adherens junction organization GO:0034332 IEA
    positive regulation of neurogenesis GO:0050769 IBA
    positive regulation of neurogenesis GO:0050769 ISS
    regulation of postsynaptic neurotransmitter receptor internalization GO:0099149 IEA
    negative regulation of protein localization to plasma membrane GO:1903077 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    early endosome GO:0005769 IEA
    plasma membrane GO:0005886 TAS
    clathrin-coated pit GO:0005905 IEA
    focal adhesion GO:0005925 HDA
    endosome membrane GO:0010008 IEA
    basolateral plasma membrane GO:0016323 IBA
    clathrin-coated vesicle GO:0030136 IDA
    cytoplasmic vesicle GO:0031410 IBA
    apical part of cell GO:0045177 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified NUMB in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for NUMB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIAH1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
2 GNL2 29889
Affinity Capture-MS Homo sapiens
3 Mdm2  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
4 GJD3  
Proximity Label-MS Homo sapiens
5 AP2B1 163
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 OCLN 100506658
Proximity Label-MS Homo sapiens
7 KIAA1107  
Affinity Capture-MS Homo sapiens
8 MARCKS 4082
Proximity Label-MS Homo sapiens
9 LAMP3  
Proximity Label-MS Homo sapiens
10 ATG9A 79065
Proximity Label-MS Homo sapiens
11 GJA1 2697
Proximity Label-MS Homo sapiens
12 SPRYD4 283377
Affinity Capture-MS Homo sapiens
13 PARD3 56288
Proximity Label-MS Homo sapiens
14 ANAPC15  
Affinity Capture-MS Homo sapiens
15 DBN1 1627
Affinity Capture-MS Homo sapiens
16 EPS15 2060
Affinity Capture-Western Homo sapiens
17 LAMP2 3920
Proximity Label-MS Homo sapiens
18 Eps15l1  
Protein-peptide Mus musculus
19 CLTB 1212
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
20 STX4 6810
Proximity Label-MS Homo sapiens
21 SOX2  
Affinity Capture-MS Homo sapiens
22 APP 351
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
23 APEX1 328
Affinity Capture-RNA Homo sapiens
24 B3GAT1  
Proximity Label-MS Homo sapiens
25 YWHAZ 7534
Affinity Capture-MS Homo sapiens
26 MLLT4 4301
Proximity Label-MS Homo sapiens
27 PKD2 5311
Affinity Capture-MS Homo sapiens
28 DPYSL2 1808
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
29 AP2A1 160
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
30 RAB3B 5865
Proximity Label-MS Homo sapiens
31 IQGAP1 8826
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
32 MLST8 64223
Affinity Capture-Western Homo sapiens
33 YWHAG 7532
Affinity Capture-MS Homo sapiens
34 Notch1  
Affinity Capture-Western Mus musculus
35 TULP3 7289
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 L1CAM 3897
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
37 LNX2  
Biochemical Activity Homo sapiens
38 GTSE1 51512
Affinity Capture-MS Homo sapiens
39 RAB35 11021
Proximity Label-MS Homo sapiens
40 MYCN  
Affinity Capture-MS Homo sapiens
41 EBAG9 9166
Proximity Label-MS Homo sapiens
42 NEDD4 4734
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
43 KIF14 9928
Affinity Capture-MS Homo sapiens
44 HSPA8 3312
Affinity Capture-MS Homo sapiens
45 LMAN1 3998
Proximity Label-MS Homo sapiens
46 SPSB3  
Affinity Capture-MS Homo sapiens
47 RAB5A 5868
Proximity Label-MS Homo sapiens
48 S100A2 6273
Affinity Capture-MS Homo sapiens
49 YWHAB 7529
Affinity Capture-MS Homo sapiens
50 CXADR 1525
Proximity Label-MS Homo sapiens
51 LYN 4067
Proximity Label-MS Homo sapiens
52 MCAM 4162
Proximity Label-MS Homo sapiens
53 RAB4A 5867
Proximity Label-MS Homo sapiens
54 DNAJC5 80331
Proximity Label-MS Homo sapiens
55 MKRN2 23609
Affinity Capture-RNA Homo sapiens
56 YWHAE 7531
Affinity Capture-MS Homo sapiens
57 DIRAS3  
Proximity Label-MS Homo sapiens
58 DCAF7 10238
Affinity Capture-MS Homo sapiens
59 DTX2 113878
Proximity Label-MS Homo sapiens
60 LAMP1 3916
Proximity Label-MS Homo sapiens
61 AP1B1 162
Affinity Capture-MS Homo sapiens
62 CDH1 999
Proximity Label-MS Homo sapiens
63 STX6 10228
Proximity Label-MS Homo sapiens
64 PDZK1IP1 10158
Two-hybrid Homo sapiens
65 SPANXN2  
Two-hybrid Homo sapiens
66 ITCH 83737
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
67 CTDSPL 10217
Proximity Label-MS Homo sapiens
68 GAK 2580
Affinity Capture-MS Homo sapiens
69 IGBP1 3476
Affinity Capture-MS Homo sapiens
70 ANLN 54443
Affinity Capture-MS Homo sapiens
71 PRKCA 5578
Affinity Capture-MS Homo sapiens
72 XPO1 7514
Affinity Capture-MS Homo sapiens
73 PANX1 24145
Proximity Label-MS Homo sapiens
74 PSENEN  
Affinity Capture-MS Homo sapiens
75 RAB11A 8766
Proximity Label-MS Homo sapiens
76 EPB41L4A 64097
Proximity Label-MS Homo sapiens
77 Trp53  
Affinity Capture-Western Mus musculus
78 ATG12  
Proximity Label-MS Homo sapiens
79 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
80 PTEN 5728
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
81 RPL3 6122
Two-hybrid Homo sapiens
82 EGFR 1956
Protein-peptide Homo sapiens
83 GLI1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
84 ARF6 382
Proximity Label-MS Homo sapiens
85 Jun  
Affinity Capture-Western Mus musculus
86 KBTBD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 AP2M1 1173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 Flnb 286940
Affinity Capture-MS Mus musculus
89 RPA3 6119
Proximity Label-MS Homo sapiens
90 MTOR 2475
Affinity Capture-Western Homo sapiens
91 RAB9A 9367
Proximity Label-MS Homo sapiens
92 COL10A1  
Affinity Capture-MS Homo sapiens
93 Eps15 13858
Protein-peptide Mus musculus
Reconstituted Complex Mus musculus
94 RHOB 388
Proximity Label-MS Homo sapiens
95 CLTC 1213
Affinity Capture-MS Homo sapiens
96 STAMBPL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 NUMBL 9253
Affinity Capture-MS Homo sapiens
98 RAB5C 5878
Proximity Label-MS Homo sapiens
99 MDM2  
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
100 FLOT1 10211
Proximity Label-MS Homo sapiens
101 RALBP1 10928
Affinity Capture-Western Homo sapiens
102 RNF43  
Proximity Label-MS Homo sapiens
103 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
104 DEPTOR 64798
Affinity Capture-Western Homo sapiens
105 LAMTOR1 55004
Proximity Label-MS Homo sapiens
106 Osgep  
Affinity Capture-MS Mus musculus
107 TP53 7157
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
108 CDC42 998
Affinity Capture-MS Homo sapiens
109 EZR 7430
Proximity Label-MS Homo sapiens
110 LCK 3932
Proximity Label-MS Homo sapiens
111 RPTOR 57521
Affinity Capture-Western Homo sapiens
112 CAPZA2 830
Affinity Capture-MS Homo sapiens
113 C11orf52 91894
Proximity Label-MS Homo sapiens
114 Myh10 77579
Affinity Capture-MS Mus musculus
115 C20orf197  
Affinity Capture-MS Homo sapiens
116 C10orf88  
Affinity Capture-MS Homo sapiens
117 RAB2A 5862
Proximity Label-MS Homo sapiens
118 SPCS1 28972
Affinity Capture-MS Homo sapiens
119 AURKA 6790
Affinity Capture-MS Homo sapiens
120 EAF1  
Two-hybrid Homo sapiens
121 ZCCHC10  
Affinity Capture-MS Homo sapiens
122 CLTA 1211
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 BEX1  
Affinity Capture-MS Homo sapiens
124 EPHA2 1969
Proximity Label-MS Homo sapiens
125 Lnx1  
Affinity Capture-Western Mus musculus
126 KRAS 3845
Proximity Label-MS Homo sapiens
127 UNC5CL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
128 SLC1A1 6505
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
129 VIM 7431
Affinity Capture-MS Homo sapiens
130 CAV1 857
Proximity Label-MS Homo sapiens
131 KLHL21 9903
Affinity Capture-MS Homo sapiens
132 NOTCH1 4851
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
133 LNX1  
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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