Gene description for FLT1
Gene name fms-related tyrosine kinase 1
Gene symbol FLT1
Other names/aliases FLT
FLT-1
VEGFR-1
VEGFR1
Species Homo sapiens
 Database cross references - FLT1
ExoCarta ExoCarta_2321
Vesiclepedia VP_2321
Entrez Gene 2321
HGNC 3763
MIM 165070
UniProt P17948  
 FLT1 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
 Gene ontology annotations for FLT1
Molecular Function
    transmembrane receptor protein tyrosine kinase activity GO:0004714 TAS
    GPI-linked ephrin receptor activity GO:0005004 IEA
    transmembrane-ephrin receptor activity GO:0005005 IEA
    epidermal growth factor receptor activity GO:0005006 IEA
    fibroblast growth factor receptor activity GO:0005007 IEA
    hepatocyte growth factor receptor activity GO:0005008 IEA
    insulin receptor activity GO:0005009 IEA
    insulin-like growth factor receptor activity GO:0005010 IEA
    macrophage colony-stimulating factor receptor activity GO:0005011 IEA
    platelet-derived growth factor alpha-receptor activity GO:0005018 IEA
    platelet-derived growth factor beta-receptor activity GO:0005019 IEA
    stem cell factor receptor activity GO:0005020 IEA
    vascular endothelial growth factor receptor activity GO:0005021 IBA
    vascular endothelial growth factor receptor activity GO:0005021 IDA
    vascular endothelial growth factor receptor activity GO:0005021 IMP
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    boss receptor activity GO:0008288 IEA
    growth factor binding GO:0019838 IBA
    growth factor binding GO:0019838 IPI
    histone H3Y41 kinase activity GO:0035401 IEA
    placental growth factor receptor activity GO:0036332 IDA
    protein tyrosine kinase collagen receptor activity GO:0038062 IEA
    brain-derived neurotrophic factor receptor activity GO:0060175 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    sprouting angiogenesis GO:0002040 IBA
    monocyte chemotaxis GO:0002548 IDA
    chromatin remodeling GO:0006338 IEA
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 IEA
    multicellular organism development GO:0007275 IBA
    positive regulation of cell population proliferation GO:0008284 TAS
    insulin receptor signaling pathway GO:0008286 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 IEA
    cell migration GO:0016477 IMP
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    cell differentiation GO:0030154 IEA
    positive regulation of cell migration GO:0030335 IBA
    positive regulation of cell migration GO:0030335 IDA
    brain-derived neurotrophic factor receptor signaling pathway GO:0031547 IEA
    platelet-derived growth factor receptor-alpha signaling pathway GO:0035790 IEA
    platelet-derived growth factor receptor-beta signaling pathway GO:0035791 IEA
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IDA
    vascular endothelial growth factor receptor-1 signaling pathway GO:0036323 IDA
    collagen-activated tyrosine kinase receptor signaling pathway GO:0038063 IEA
    vascular endothelial growth factor signaling pathway GO:0038084 IEA
    Kit signaling pathway GO:0038109 IEA
    macrophage colony-stimulating factor signaling pathway GO:0038145 IEA
    positive regulation of MAP kinase activity GO:0043406 IDA
    positive regulation of MAPK cascade GO:0043410 IBA
    positive regulation of MAPK cascade GO:0043410 IDA
    positive regulation of angiogenesis GO:0045766 IMP
    protein autophosphorylation GO:0046777 IDA
    insulin-like growth factor receptor signaling pathway GO:0048009 IEA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 IBA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 IDA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 IMP
    hepatocyte growth factor receptor signaling pathway GO:0048012 IEA
    ephrin receptor signaling pathway GO:0048013 IEA
    blood vessel morphogenesis GO:0048514 ISS
    embryonic morphogenesis GO:0048598 ISS
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IMP
    negative regulation of vascular endothelial cell proliferation GO:1905563 IGI
    hyaloid vascular plexus regression GO:1990384 ISS
Subcellular Localization
    extracellular space GO:0005615 TAS
    endosome GO:0005768 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    focal adhesion GO:0005925 HDA
    actin cytoskeleton GO:0015629 IDA
    receptor complex GO:0043235 IBA
    receptor complex GO:0043235 IDA
 Experiment description of studies that identified FLT1 in sEVs
1
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for FLT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCL3  
Affinity Capture-MS Homo sapiens
2 PTPN11 5781
Co-localization Homo sapiens
Co-localization Homo sapiens
3 NEDD4 4734
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
4 SHC1 6464
Two-hybrid Homo sapiens
5 CRK 1398
Co-localization Homo sapiens
6 CTNNB1 1499
Co-localization Homo sapiens
7 VEGFA 7422
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 HCK 3055
Co-localization Homo sapiens
9 CBL 867
Affinity Capture-Western Homo sapiens
10 FLT1 2321
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
11 FBXO2 26232
Affinity Capture-MS Homo sapiens
12 Nedd4 25489
Biochemical Activity Rattus norvegicus
13 CRKL 1399
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
14 SCGB2A2  
Affinity Capture-MS Homo sapiens
15 C1orf54  
Affinity Capture-MS Homo sapiens
16 PGF  
Reconstituted Complex Homo sapiens
17 VEGFB  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
18 PTK2 5747
Co-localization Homo sapiens
19 RPL36 25873
Affinity Capture-MS Homo sapiens
20 PIK3R1 5295
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
21 KDR  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
22 PLCG1 5335
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Co-localization Homo sapiens
23 ZNF467  
Affinity Capture-MS Homo sapiens
24 SRPK1 6732
Biochemical Activity Homo sapiens
25 SHC2 25759
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
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