Gene description for H3F3A
Gene name H3 histone, family 3A
Gene symbol H3F3A
Other names/aliases H3.3A
H3F3
Species Homo sapiens
 Database cross references - H3F3A
ExoCarta ExoCarta_3020
Vesiclepedia VP_3020
Entrez Gene 3020
HGNC 4764
MIM 601128
UniProt P84243  
 H3F3A identified in sEVs derived from the following tissue/cell type
Bladder cancer cells 20224111    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for H3F3A
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    RNA polymerase II core promoter sequence-specific DNA binding GO:0000979 IDA
    protein binding GO:0005515 IPI
    structural constituent of chromatin GO:0030527 IDA
    nucleosomal DNA binding GO:0031492 IDA
    protein heterodimerization activity GO:0046982 IEA
Biological Process
    oocyte maturation GO:0001556 IEA
    osteoblast differentiation GO:0001649 IEA
    nucleosome assembly GO:0006334 IDA
    nucleosome assembly GO:0006334 IMP
    nucleus organization GO:0006997 IEA
    spermatid development GO:0007286 IEA
    single fertilization GO:0007338 IEA
    embryo implantation GO:0007566 IEA
    cell population proliferation GO:0008283 IEA
    male gonad development GO:0008584 IEA
    positive regulation of cell growth GO:0030307 IMP
    pericentric heterochromatin formation GO:0031508 IEA
    subtelomeric heterochromatin formation GO:0031509 IEA
    telomere organization GO:0032200 TAS
    multicellular organism growth GO:0035264 IEA
    muscle cell differentiation GO:0042692 IEA
    regulation of centromere complex assembly GO:0090230 IEA
    negative regulation of chromosome condensation GO:1902340 IEA
Subcellular Localization
    chromosome, telomeric region GO:0000781 IDA
    nucleosome GO:0000786 IDA
    inner kinetochore GO:0000939 IEA
    Barr body GO:0001740 IEA
    extracellular region GO:0005576 TAS
    nucleus GO:0005634 HDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IMP
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    protein-containing complex GO:0032991 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified H3F3A in sEVs
1
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
16
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
17
Experiment ID 138
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name DU145 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
18
Experiment ID 143
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name LNCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
19
Experiment ID 144
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
20
Experiment ID 145
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD10
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
21
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for H3F3A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDCA5  
Affinity Capture-MS Homo sapiens
2 Wdr48  
Affinity Capture-MS Mus musculus
3 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 VPS35 55737
Co-fractionation Homo sapiens
6 UBE2H 7328
Affinity Capture-MS Homo sapiens
7 HNRNPC 3183
Co-fractionation Homo sapiens
8 CBX1 10951
Affinity Capture-MS Homo sapiens
9 PPP1CB 5500
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 DNAJC11 55735
Affinity Capture-MS Homo sapiens
11 VARS 7407
Co-fractionation Homo sapiens
12 MSH6 2956
Affinity Capture-MS Homo sapiens
13 Rcc1  
Affinity Capture-MS Mus musculus
14 PAWR 5074
Co-fractionation Homo sapiens
15 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
16 DHRS12  
Affinity Capture-MS Homo sapiens
17 HIST1H1D 3007
Cross-Linking-MS (XL-MS) Homo sapiens
18 BTF3 689
Affinity Capture-MS Homo sapiens
19 VPRBP 9730
Reconstituted Complex Homo sapiens
20 H2AFJ 55766
Cross-Linking-MS (XL-MS) Homo sapiens
21 CABIN1  
Affinity Capture-Western Homo sapiens
22 HIST1H2BJ 8970
Affinity Capture-MS Homo sapiens
23 RPS19 6223
Co-fractionation Homo sapiens
24 UBC 7316
Cross-Linking-MS (XL-MS) Homo sapiens
25 TROVE2 6738
Co-fractionation Homo sapiens
26 KMT2C 58508
Affinity Capture-MS Homo sapiens
27 KDM4A  
Co-crystal Structure Homo sapiens
28 SNX6 58533
Co-fractionation Homo sapiens
29 PRKDC 5591
Affinity Capture-MS Homo sapiens
30 Nhsl1  
Affinity Capture-MS Mus musculus
31 VPS29 51699
Co-fractionation Homo sapiens
32 CENPA  
Co-purification Homo sapiens
Co-purification Homo sapiens
33 LRPPRC 10128
Co-fractionation Homo sapiens
34 VPS26A 9559
Co-fractionation Homo sapiens
35 DDX23 9416
Proximity Label-MS Homo sapiens
36 TOP2A 7153
Affinity Capture-MS Homo sapiens
37 TRIM28 10155
Affinity Capture-MS Homo sapiens
38 CHAF1A  
Affinity Capture-Western Homo sapiens
39 MCM2 4171
Affinity Capture-MS Homo sapiens
40 KPNA3 3839
Co-fractionation Homo sapiens
41 SF3B1 23451
Affinity Capture-MS Homo sapiens
42 BRWD3  
Affinity Capture-MS Homo sapiens
43 SPAG9 9043
Co-fractionation Homo sapiens
44 HIST2H3A 333932
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
45 SUV39H1  
Biochemical Activity Homo sapiens
46 ZMYND11 10771
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
47 C4orf27  
Reconstituted Complex Homo sapiens
48 ASB9  
Affinity Capture-MS Homo sapiens
49 HIST4H4 121504
Affinity Capture-Western Homo sapiens
50 FANCD2  
Affinity Capture-MS Homo sapiens
51 HIST2H2AC 8338
Cross-Linking-MS (XL-MS) Homo sapiens
52 ITGA4 3676
Affinity Capture-MS Homo sapiens
53 ATRX 546
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
54 NASP 4678
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
55 FBL 2091
Affinity Capture-MS Homo sapiens
56 RAB35 11021
Proximity Label-MS Homo sapiens
57 MYCN  
Affinity Capture-MS Homo sapiens
58 ASF1A 25842
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
59 FGFR1 2260
Co-localization Homo sapiens
60 NEDD4 4734
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
61 FGF2 2247
Co-localization Homo sapiens
62 TFAP2A  
Affinity Capture-Western Homo sapiens
63 PPM1D  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
64 TH  
Co-localization Homo sapiens
65 PPP1CA 5499
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
66 VRK3 51231
Affinity Capture-MS Homo sapiens
67 KIAA0020 9933
Cross-Linking-MS (XL-MS) Homo sapiens
68 RNF41  
Affinity Capture-MS Homo sapiens
69 RNF4 6047
Affinity Capture-MS Homo sapiens
70 TOP3B 8940
Affinity Capture-MS Homo sapiens
71 KPNA4 3840
Affinity Capture-MS Homo sapiens
72 EMD 2010
Proximity Label-MS Homo sapiens
73 CDKN1A  
Co-localization Homo sapiens
74 PARK2  
Affinity Capture-MS Homo sapiens
75 HIST1H2AG 8969
Cross-Linking-MS (XL-MS) Homo sapiens
76 DNMT3A 1788
Co-crystal Structure Homo sapiens
77 CBX3 11335
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
78 VRK1 7443
Affinity Capture-MS Homo sapiens
79 TSPYL5  
Affinity Capture-MS Homo sapiens
80 CARM1 10498
Affinity Capture-MS Homo sapiens
81 NUDC 10726
Co-fractionation Homo sapiens
82 MYH9 4627
Co-fractionation Homo sapiens
83 VCP 7415
Affinity Capture-MS Homo sapiens
84 CKAP5 9793
Affinity Capture-MS Homo sapiens
85 CBX5 23468
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 U2AF2 11338
Co-fractionation Homo sapiens
87 BPTF 2186
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
88 DNAJA1 3301
Co-fractionation Homo sapiens
89 CAMKK1  
Affinity Capture-MS Homo sapiens
90 HIST1H1C 3006
Cross-Linking-MS (XL-MS) Homo sapiens
91 PML 5371
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
92 BRD4 23476
Reconstituted Complex Homo sapiens
93 PELP1 27043
Reconstituted Complex Homo sapiens
94 HDAC5 10014
Affinity Capture-MS Homo sapiens
95 CEBPA  
Protein-peptide Homo sapiens
96 SETD7 80854
Biochemical Activity Homo sapiens
97 DAXX  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
98 HDAC9  
Affinity Capture-MS Homo sapiens
99 ear  
Reconstituted Complex Drosophila melanogaster
100 BBC3  
Co-localization Homo sapiens
101 BRD7  
Reconstituted Complex Homo sapiens
102 HIST1H2BL 8340
Cross-Linking-MS (XL-MS) Homo sapiens
103 SF3B2 10992
Affinity Capture-MS Homo sapiens
104 FAM129B 64855
Co-fractionation Homo sapiens
105 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
106 Myh9 17886
Affinity Capture-MS Mus musculus
107 KMT2A  
Biochemical Activity Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
108 HIST2H3C 126961
Affinity Capture-MS Homo sapiens
109 NOS3  
Two-hybrid Homo sapiens
110 PDGFRA 5156
Affinity Capture-MS Homo sapiens
111 POLK  
Affinity Capture-MS Homo sapiens
112 CUL3 8452
Affinity Capture-MS Homo sapiens
113 UBR7  
Affinity Capture-Western Homo sapiens
114 HIST1H2BH 8345
Cross-Linking-MS (XL-MS) Homo sapiens
115 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
116 FUS 2521
Affinity Capture-MS Homo sapiens
117 EPRS 2058
Cross-Linking-MS (XL-MS) Homo sapiens
118 CORO1C 23603
Co-fractionation Homo sapiens
119 RBBP4 5928
Affinity Capture-MS Homo sapiens
120 HIST3H3 8290
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
121 ASB16 92591
Affinity Capture-MS Homo sapiens
122 PRMT5 10419
Co-fractionation Homo sapiens
123 HIST1H1B 3009
Affinity Capture-MS Homo sapiens
124 MYC  
Affinity Capture-MS Homo sapiens
125 KIF11 3832
Affinity Capture-MS Homo sapiens
126 SET 6418
Reconstituted Complex Homo sapiens
127 TOP1 7150
Affinity Capture-MS Homo sapiens
128 EHMT2 10919
Affinity Capture-MS Homo sapiens
129 Cep78  
Affinity Capture-MS Mus musculus
130 HIST1H4F 8361
Affinity Capture-Western Homo sapiens
131 HIST1H1A 3024
Cross-Linking-MS (XL-MS) Homo sapiens
132 RNF2  
Affinity Capture-MS Homo sapiens
133 RHOB 388
Proximity Label-MS Homo sapiens
134 HAT1 8520
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
135 ASH1L 55870
Affinity Capture-MS Homo sapiens
136 HIST1H1T 3010
Cross-Linking-MS (XL-MS) Homo sapiens
137 SUPT16H 11198
Affinity Capture-MS Homo sapiens
138 H3F3C 440093
Cross-Linking-MS (XL-MS) Homo sapiens
139 XRCC5 7520
Affinity Capture-MS Homo sapiens
140 CCDC71  
Affinity Capture-MS Homo sapiens
141 H2AFX 3014
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
142 Lgals3bp 19039
Affinity Capture-MS Mus musculus
143 CBX4  
Affinity Capture-MS Homo sapiens
144 DNMT3L  
Co-crystal Structure Homo sapiens
145 RPS27A 6233
Cross-Linking-MS (XL-MS) Homo sapiens
146 WDR34 89891
Affinity Capture-MS Homo sapiens
147 RSF1  
Affinity Capture-MS Homo sapiens
148 PARP1 142
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
149 KAT2A  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
150 HMGB1 3146
Affinity Capture-MS Homo sapiens
151 SAP130  
Affinity Capture-MS Homo sapiens
152 FOLR1 2348
Affinity Capture-MS Homo sapiens
153 ASF1B  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
154 XRCC6 2547
Affinity Capture-MS Homo sapiens
155 MCM5 4174
Affinity Capture-MS Homo sapiens
156 KAT2B  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
157 EZR 7430
Proximity Label-MS Homo sapiens
158 RPA1 6117
Affinity Capture-Western Homo sapiens
159 UBA52 7311
Cross-Linking-MS (XL-MS) Homo sapiens
160 PDHA1 5160
Affinity Capture-MS Homo sapiens
161 KDM4C  
Affinity Capture-MS Homo sapiens
162 SMURF1 57154
Affinity Capture-MS Homo sapiens
163 THRAP3 9967
Affinity Capture-MS Homo sapiens
164 HIST1H1E 3008
Cross-Linking-MS (XL-MS) Homo sapiens
165 Mllt3  
Reconstituted Complex Mus musculus
166 HDAC1 3065
Affinity Capture-MS Homo sapiens
167 CHD4 1108
Affinity Capture-MS Homo sapiens
168 PUF60 22827
Co-fractionation Homo sapiens
169 H2AFY 9555
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
170 HNRNPK 3190
Affinity Capture-MS Homo sapiens
171 PARP2  
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
172 ESR1  
Reconstituted Complex Homo sapiens
173 UFL1 23376
Affinity Capture-MS Homo sapiens
174 UBB 7314
Cross-Linking-MS (XL-MS) Homo sapiens
175 NCL 4691
Affinity Capture-MS Homo sapiens
176 LRRK2 120892
Affinity Capture-MS Homo sapiens
177 IPO4 79711
Affinity Capture-MS Homo sapiens
178 PHACTR3  
Affinity Capture-MS Homo sapiens
179 AP4M1 9179
Affinity Capture-MS Homo sapiens
180 HIST1H4A 8359
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
181 BRD1 23774
Reconstituted Complex Homo sapiens
182 MLLT3 4300
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
183 ASB2  
Affinity Capture-MS Homo sapiens
184 EHMT1  
Affinity Capture-MS Homo sapiens
185 HIRA  
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
186 SNX2 6643
Co-fractionation Homo sapiens
187 KRAS 3845
Negative Genetic Homo sapiens
188 RAB5C 5878
Affinity Capture-MS Homo sapiens
189 C9orf72  
Affinity Capture-MS Homo sapiens
190 AR 367
Affinity Capture-MS Homo sapiens
191 UBN1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
192 SSRP1 6749
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
193 RPA2 6118
Affinity Capture-Western Homo sapiens
194 WDR60 55112
Affinity Capture-MS Homo sapiens
195 HIST1H2BD 3017
Cross-Linking-MS (XL-MS) Homo sapiens
196 NEDD8 4738
Cross-Linking-MS (XL-MS) Homo sapiens
197 EP300 2033
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which H3F3A is involved
PathwayEvidenceSource
Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 TAS Reactome
Activation of anterior HOX genes in hindbrain development during early embryogenesis IEA Reactome
Activation of HOX genes during differentiation IEA Reactome
Amyloid fiber formation TAS Reactome
Assembly of the ORC complex at the origin of replication TAS Reactome
Assembly of the pre-replicative complex TAS Reactome
B-WICH complex positively regulates rRNA expression TAS Reactome
Cell Cycle IEA Reactome
Cell Cycle TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cellular Senescence TAS Reactome
Chromatin modifications during the maternal to zygotic transition (MZT) IEA Reactome
Chromosome Maintenance TAS Reactome
Condensation of Prophase Chromosomes TAS Reactome
Defective pyroptosis TAS Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
Diseases of programmed cell death TAS Reactome
DNA methylation IEA Reactome
DNA Replication TAS Reactome
DNA Replication Pre-Initiation TAS Reactome
Epigenetic regulation by WDR5-containing histone modifying complexes IEA Reactome
Epigenetic regulation by WDR5-containing histone modifying complexes TAS Reactome
Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes IEA Reactome
Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes TAS Reactome
Epigenetic regulation of gene expression TAS Reactome
Epigenetic regulation of gene expression IEA Reactome
Epigenetic regulation of gene expression by MLL3 and MLL4 complexes IEA Reactome
Epigenetic regulation of gene expression by MLL3 and MLL4 complexes TAS Reactome
ERCC6 (CSB) and EHMT2 (G9a) positively regulate rRNA expression IEA Reactome
ESR-mediated signaling TAS Reactome
Estrogen-dependent gene expression TAS Reactome
Factors involved in megakaryocyte development and platelet production TAS Reactome
Formation of the beta-catenin:TCF transactivating complex TAS Reactome
Gene expression (Transcription) TAS Reactome
Gene expression (Transcription) IEA Reactome
Gene Silencing by RNA TAS Reactome
Generic Transcription Pathway TAS Reactome
Generic Transcription Pathway IEA Reactome
Hemostasis TAS Reactome
Inhibition of DNA recombination at telomere TAS Reactome
M Phase TAS Reactome
Maternal to zygotic transition (MZT) IEA Reactome
Meiosis IEA Reactome
Meiotic recombination IEA Reactome
Metabolism of proteins TAS Reactome
Mitotic Prophase TAS Reactome
MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis IEA Reactome
MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis TAS Reactome
Negative epigenetic regulation of rRNA expression TAS Reactome
Negative epigenetic regulation of rRNA expression IEA Reactome
NoRC negatively regulates rRNA expression IEA Reactome
NoRC negatively regulates rRNA expression TAS Reactome
Oxidative Stress Induced Senescence TAS Reactome
Positive epigenetic regulation of rRNA expression TAS Reactome
Positive epigenetic regulation of rRNA expression IEA Reactome
PRC2 methylates histones and DNA TAS Reactome
Pre-NOTCH Expression and Processing IEA Reactome
Pre-NOTCH Transcription and Translation IEA Reactome
Regulation of endogenous retroelements TAS Reactome
Regulation of endogenous retroelements IEA Reactome
Regulation of endogenous retroelements by KRAB-ZFP proteins TAS Reactome
Regulation of endogenous retroelements by KRAB-ZFP proteins IEA Reactome
Regulation of endogenous retroelements by Piwi-interacting RNAs (piRNAs) IEA Reactome
Regulation of endogenous retroelements by the Human Silencing Hub (HUSH) complex TAS Reactome
Regulation of endogenous retroelements by the Human Silencing Hub (HUSH) complex IEA Reactome
Replacement of protamines by nucleosomes in the male pronucleus IEA Reactome
Reproduction IEA Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPases activate PKNs TAS Reactome
RNA Polymerase I Promoter Clearance TAS Reactome
RNA Polymerase I Promoter Escape TAS Reactome
RNA Polymerase I Promoter Opening TAS Reactome
RNA Polymerase I Transcription TAS Reactome
RNA Polymerase II Transcription TAS Reactome
RNA Polymerase II Transcription IEA Reactome
RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function TAS Reactome
RUNX1 regulates transcription of genes involved in differentiation of HSCs IEA Reactome
Senescence-Associated Secretory Phenotype (SASP) TAS Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by NOTCH IEA Reactome
Signaling by Nuclear Receptors TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by WNT TAS Reactome
SIRT1 negatively regulates rRNA expression TAS Reactome
TCF dependent signaling in response to WNT TAS Reactome
Telomere Maintenance TAS Reactome
Transcriptional regulation by RUNX1 TAS Reactome
Transcriptional regulation by RUNX1 IEA Reactome
Transcriptional regulation by small RNAs TAS Reactome
Transcriptional regulation of granulopoiesis IEA Reactome





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