Gene description for MUT
Gene name methylmalonyl CoA mutase
Gene symbol MUT
Other names/aliases MCM
Species Homo sapiens
 Database cross references - MUT
ExoCarta ExoCarta_4594
Vesiclepedia VP_4594
Entrez Gene 4594
HGNC 7526
MIM 609058
UniProt P22033  
 MUT identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MUT
Molecular Function
    GTPase activity GO:0003924 IDA
    methylmalonyl-CoA mutase activity GO:0004494 IBA
    methylmalonyl-CoA mutase activity GO:0004494 IDA
    methylmalonyl-CoA mutase activity GO:0004494 IMP
    protein binding GO:0005515 IPI
    cobalamin binding GO:0031419 IBA
    cobalamin binding GO:0031419 IDA
    identical protein binding GO:0042802 IDA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    metal ion binding GO:0046872 IEA
    modified amino acid binding GO:0072341 IDA
Biological Process
    post-embryonic development GO:0009791 IEA
    propionate metabolic process, methylmalonyl pathway GO:0019678 IBA
    positive regulation of GTPase activity GO:0043547 IDA
    homocysteine metabolic process GO:0050667 IDA
    succinyl-CoA biosynthetic process GO:1901290 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 IDA
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified MUT in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MUT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDHA1 5160
Proximity Label-MS Homo sapiens
2 Esrrb  
Affinity Capture-MS Mus musculus
3 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
4 BPHL 670
Affinity Capture-MS Homo sapiens
5 GRPEL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 KLF15  
Affinity Capture-MS Homo sapiens
7 AUH 549
Proximity Label-MS Homo sapiens
8 KIF14 9928
Affinity Capture-MS Homo sapiens
9 AK4 205
Affinity Capture-MS Homo sapiens
10 THUMPD1 55623
Co-fractionation Homo sapiens
11 L3HYPDH 112849
Affinity Capture-MS Homo sapiens
12 DHTKD1 55526
Co-fractionation Homo sapiens
13 MDH2 4191
Proximity Label-MS Homo sapiens
14 OXLD1  
Affinity Capture-MS Homo sapiens
15 TMEM184A  
Affinity Capture-MS Homo sapiens
16 SLC25A10 1468
Affinity Capture-MS Homo sapiens
17 MTG2  
Proximity Label-MS Homo sapiens
18 MMAA  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
19 SF1 7536
Co-fractionation Homo sapiens
20 ACSM5 54988
Affinity Capture-MS Homo sapiens
21 NAA50 80218
Co-fractionation Homo sapiens
22 CYP1A1  
Affinity Capture-MS Homo sapiens
23 FAHD1 81889
Affinity Capture-MS Homo sapiens
24 ICT1 3396
Affinity Capture-MS Homo sapiens
25 MUT 4594
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
26 PTK2 5747
Co-fractionation Homo sapiens
27 CNPY3 10695
Co-fractionation Homo sapiens
28 AMACR 23600
Affinity Capture-MS Homo sapiens
29 MTIF2 4528
Proximity Label-MS Homo sapiens
30 SSBP1 6742
Proximity Label-MS Homo sapiens
31 CCDC90B  
Proximity Label-MS Homo sapiens
32 CRYZ 1429
Affinity Capture-MS Homo sapiens
33 RPA3 6119
Proximity Label-MS Homo sapiens
34 NTRK1 4914
Affinity Capture-MS Homo sapiens
35 ACAD9 28976
Proximity Label-MS Homo sapiens
36 YARS2  
Affinity Capture-MS Homo sapiens
37 MRPS24 64951
Affinity Capture-MS Homo sapiens
38 TPRKB 51002
Co-fractionation Homo sapiens
39 METTL17  
Proximity Label-MS Homo sapiens
40 DDX58 23586
Affinity Capture-RNA Homo sapiens
41 CFH 3075
Affinity Capture-MS Homo sapiens
42 CSTF3 1479
Co-fractionation Homo sapiens
43 MCUR1 63933
Proximity Label-MS Homo sapiens
44 ARHGEF26 26084
Affinity Capture-MS Homo sapiens
45 PEPD 5184
Co-fractionation Homo sapiens
46 CD2AP 23607
Co-fractionation Homo sapiens
47 RALB 5899
Co-fractionation Homo sapiens
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