Gene description for MX1
Gene name MX dynamin-like GTPase 1
Gene symbol MX1
Other names/aliases IFI-78K
IFI78
MX
MxA
Species Homo sapiens
 Database cross references - MX1
ExoCarta ExoCarta_4599
Vesiclepedia VP_4599
Entrez Gene 4599
HGNC 7532
MIM 147150
UniProt P20591  
 MX1 identified in sEVs derived from the following tissue/cell type
Bladder cancer cells 20224111    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Thymus 23844026    
 Gene ontology annotations for MX1
Molecular Function
    GTPase activity GO:0003924 IBA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    microtubule binding GO:0008017 IBA
    identical protein binding GO:0042802 IPI
Biological Process
    apoptotic process GO:0006915 TAS
    defense response GO:0006952 TAS
    signal transduction GO:0007165 TAS
    response to virus GO:0009615 IMP
    synaptic vesicle budding from presynaptic endocytic zone membrane GO:0016185 IBA
    receptor internalization GO:0031623 IBA
    response to type I interferon GO:0034340 TAS
    negative regulation of viral genome replication GO:0045071 IDA
    innate immune response GO:0045087 TAS
    defense response to virus GO:0051607 IBA
    defense response to virus GO:0051607 IDA
    interleukin-27-mediated signaling pathway GO:0070106 IEP
    antiviral innate immune response GO:0140374 IEP
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    cytosol GO:0005829 HDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IBA
    plasma membrane GO:0005886 IBA
    nuclear membrane GO:0031965 IDA
    synapse GO:0045202 IBA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified MX1 in sEVs
1
Experiment ID 76
MISEV standards
EM
Biophysical techniques
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
HSP90B1
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 140
MISEV standards
EM
Biophysical techniques
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
HSP90B1
Negative markers
Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name VCaP - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
3
Experiment ID 141
MISEV standards
EM
Biophysical techniques
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
HSP90B1
Negative markers
Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name VCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
4
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MX1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIAH1  
Two-hybrid Homo sapiens
2 APP 351
Reconstituted Complex Homo sapiens
3 RELA 5970
Two-hybrid Homo sapiens
4 CBX4  
Two-hybrid Homo sapiens
5 BCS1L 617
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 ISG15 9636
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
7 PSD3 23362
Two-hybrid Homo sapiens
8 EPM2AIP1 9852
Two-hybrid Homo sapiens
9 C18orf21  
Affinity Capture-MS Homo sapiens
10 KLHL35  
Two-hybrid Homo sapiens
11 SLC25A3 5250
Two-hybrid Homo sapiens
12 PLRG1 5356
Two-hybrid Homo sapiens
13 SIRPA 140885
Reconstituted Complex Homo sapiens
14 ZNF623  
Two-hybrid Homo sapiens
15 MX1 4599
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-purification Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-purification Homo sapiens
Two-hybrid Homo sapiens
16 ZBTB16  
Two-hybrid Homo sapiens
17 DDX58 23586
Affinity Capture-RNA Homo sapiens
18 BLM 641
Two-hybrid Homo sapiens
19 TUBA1A 7846
Two-hybrid Homo sapiens
20 KIF26B  
Two-hybrid Homo sapiens
21 HMGXB4 10042
Two-hybrid Homo sapiens
22 SRRT 51593
Affinity Capture-MS Homo sapiens
23 FANCA  
Two-hybrid Homo sapiens
24 UBE2I 7329
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
25 PIAS1 8554
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
26 SIK1  
Affinity Capture-MS Homo sapiens
27 CHD3 1107
Two-hybrid Homo sapiens
28 GMEB1  
Two-hybrid Homo sapiens
29 MCCD1  
Two-hybrid Homo sapiens
30 BRD7  
Two-hybrid Homo sapiens
31 SSUH2  
Affinity Capture-MS Homo sapiens
32 C7orf25  
Two-hybrid Homo sapiens
33 ADRM1 11047
Two-hybrid Homo sapiens
34 CASP8AP2  
Two-hybrid Homo sapiens
35 TDG 6996
Two-hybrid Homo sapiens
36 ZNF251  
Two-hybrid Homo sapiens
37 CAB39L 81617
Two-hybrid Homo sapiens
38 LRRC4B  
Two-hybrid Homo sapiens
39 ZCCHC12  
Two-hybrid Homo sapiens
40 DAXX  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
41 SUMO1 7341
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
42 SP100 6672
Two-hybrid Homo sapiens
43 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
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