Gene description for PECAM1
Gene name platelet/endothelial cell adhesion molecule 1
Gene symbol PECAM1
Other names/aliases CD31
CD31/EndoCAM
GPIIA'
PECA1
PECAM-1
endoCAM
Species Homo sapiens
 Database cross references - PECAM1
ExoCarta ExoCarta_5175
Vesiclepedia VP_5175
Entrez Gene 5175
HGNC 8823
MIM 173445
UniProt P16284  
 PECAM1 identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for PECAM1
Molecular Function
    transmembrane signaling receptor activity GO:0004888 IBA
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IDA
Biological Process
    positive regulation of protein phosphorylation GO:0001934 IMP
    phagocytosis GO:0006909 IDA
    immune response GO:0006955 IBA
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IMP
    leukocyte cell-cell adhesion GO:0007159 IMP
    signal transduction GO:0007165 TAS
    cell surface receptor signaling pathway GO:0007166 IBA
    cell recognition GO:0008037 TAS
    positive regulation of cell migration GO:0030335 IDA
    maintenance of blood-brain barrier GO:0035633 NAS
    monocyte extravasation GO:0035696 IMP
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IMP
    diapedesis GO:0050904 IDA
    establishment of endothelial barrier GO:0061028 IMP
    bicellular tight junction assembly GO:0070830 IMP
    glomerular endothelium development GO:0072011 IEP
    neutrophil extravasation GO:0072672 IMP
    cell-cell adhesion GO:0098609 IDA
    cell-cell adhesion via plasma-membrane adhesion molecules GO:0098742 IBA
    cell-cell adhesion via plasma-membrane adhesion molecules GO:0098742 IMP
    positive regulation of protein localization to cell-cell junction GO:0150107 IDA
Subcellular Localization
    extracellular space GO:0005615 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IDA
    external side of plasma membrane GO:0009897 IBA
    secretory granule membrane GO:0030667 TAS
    platelet alpha granule membrane GO:0031092 TAS
    protein-containing complex GO:0032991 IDA
    membrane raft GO:0045121 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified PECAM1 in sEVs
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PECAM1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTPN11 5781
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 JUP 3728
Affinity Capture-Western Homo sapiens
3 CSK 1445
Biochemical Activity Homo sapiens
4 PTPN6 5777
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 MAPK1 5594
Affinity Capture-Western Homo sapiens
6 CTNNB1 1499
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
7 ITGB3 3690
Co-localization Homo sapiens
8 LCK 3932
Biochemical Activity Homo sapiens
9 LGALS1 3956
Affinity Capture-MS Homo sapiens
10 HCK 3055
Affinity Capture-Western Homo sapiens
11 SYVN1 84447
Co-localization Homo sapiens
12 PRPF40A 55660
Two-hybrid Homo sapiens
13 LYN 4067
Affinity Capture-Western Homo sapiens
14 ADD2 119
Affinity Capture-MS Homo sapiens
15 RBM12 10137
Affinity Capture-MS Homo sapiens
16 FYN 2534
Affinity Capture-Western Homo sapiens
17 YES1 7525
Affinity Capture-Western Homo sapiens
18 XRCC6 2547
Two-hybrid Homo sapiens
19 SRC 6714
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 PIK3R1 5295
Affinity Capture-Western Homo sapiens
21 PPP2R1A 5518
Affinity Capture-Western Homo sapiens
22 INPP5D 3635
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
23 PLCG1 5335
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
24 PECAM1 5175
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
25 DSP 1832
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here