Gene description for ERAP1
Gene name endoplasmic reticulum aminopeptidase 1
Gene symbol ERAP1
Other names/aliases A-LAP
ALAP
APPILS
ARTS-1
ARTS1
ERAAP
ERAAP1
PILS-AP
PILSAP
Species Homo sapiens
 Database cross references - ERAP1
ExoCarta ExoCarta_51752
Vesiclepedia VP_51752
Entrez Gene 51752
HGNC 18173
MIM 606832
UniProt Q9NZ08  
 ERAP1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Saliva 19199708    
Thymus 23844026    
 Gene ontology annotations for ERAP1
Molecular Function
    endopeptidase activity GO:0004175 EXP
    aminopeptidase activity GO:0004177 IDA
    interleukin-6 receptor binding GO:0005138 IPI
    interleukin-1, type II receptor binding GO:0005151 TAS
    protein binding GO:0005515 IPI
    metalloexopeptidase activity GO:0008235 IDA
    zinc ion binding GO:0008270 IBA
    zinc ion binding GO:0008270 NAS
    peptide binding GO:0042277 IBA
    metalloaminopeptidase activity GO:0070006 IBA
Biological Process
    angiogenesis GO:0001525 TAS
    adaptive immune response GO:0002250 IEA
    antigen processing and presentation of peptide antigen via MHC class I GO:0002474 TAS
    proteolysis GO:0006508 IBA
    membrane protein ectodomain proteolysis GO:0006509 IDA
    regulation of blood pressure GO:0008217 NAS
    regulation of blood pressure GO:0008217 TAS
    response to bacterium GO:0009617 NAS
    antigen processing and presentation of endogenous peptide antigen via MHC class I GO:0019885 NAS
    antigen processing and presentation of endogenous peptide antigen via MHC class I GO:0019885 TAS
    peptide catabolic process GO:0043171 IBA
    regulation of innate immune response GO:0045088 NAS
    fat cell differentiation GO:0045444 NAS
    positive regulation of angiogenesis GO:0045766 IEA
Subcellular Localization
    extracellular region GO:0005576 IDA
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    cytoplasm GO:0005737 IBA
    endoplasmic reticulum GO:0005783 NAS
    endoplasmic reticulum lumen GO:0005788 TAS
    endoplasmic reticulum membrane GO:0005789 IEA
    cytosol GO:0005829 NAS
    membrane GO:0016020 HDA
    membrane GO:0016020 IBA
    membrane GO:0016020 NAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ERAP1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ERAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD14 929
Affinity Capture-MS Homo sapiens
2 GPR17 2840
Affinity Capture-MS Homo sapiens
3 SLC16A10 117247
Affinity Capture-MS Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 SLC15A1  
Affinity Capture-MS Homo sapiens
6 APLNR  
Affinity Capture-MS Homo sapiens
7 CXCR4 7852
Affinity Capture-MS Homo sapiens
8 SIAE 54414
Affinity Capture-MS Homo sapiens
9 DEFA1 1667
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 DHCR24 1718
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 LRRC59 55379
Proximity Label-MS Homo sapiens
12 OPALIN  
Affinity Capture-MS Homo sapiens
13 PDIA4 9601
Proximity Label-MS Homo sapiens
14 KLK11 11012
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SCGB1D4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 HLA-B 3106
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 ADRB2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
18 TMEM205 374882
Affinity Capture-MS Homo sapiens
19 FPR1  
Affinity Capture-MS Homo sapiens
20 PARK2  
Affinity Capture-MS Homo sapiens
21 FBXO6 26270
Affinity Capture-MS Homo sapiens
22 PLD4  
Affinity Capture-MS Homo sapiens
23 AQP3  
Affinity Capture-MS Homo sapiens
24 MFSD4  
Affinity Capture-MS Homo sapiens
25 P2RY8  
Affinity Capture-MS Homo sapiens
26 HIST3H3 8290
Protein-peptide Homo sapiens
27 INS 3630
Affinity Capture-MS Homo sapiens
28 NXF1 10482
Affinity Capture-RNA Homo sapiens
29 KIR2DS2  
Affinity Capture-MS Homo sapiens
30 MUL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 MOV10 4343
Affinity Capture-RNA Homo sapiens
32 GLUL 2752
Co-fractionation Homo sapiens
33 KRTAP19-6  
Affinity Capture-MS Homo sapiens
34 GPR45  
Affinity Capture-MS Homo sapiens
35 ROMO1 140823
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 GPR182  
Affinity Capture-MS Homo sapiens
37 TMEM87A 25963
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 YES1 7525
Co-fractionation Homo sapiens
39 HLA-DQB2  
Affinity Capture-MS Homo sapiens
40 ENO1 2023
Affinity Capture-RNA Homo sapiens
41 CTSB 1508
Co-fractionation Homo sapiens
42 CCR6  
Affinity Capture-MS Homo sapiens
43 APOL2 23780
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 SLAMF1 6504
Affinity Capture-MS Homo sapiens
45 FAM124B  
Affinity Capture-MS Homo sapiens
46 SEC61B 10952
Proximity Label-MS Homo sapiens
47 ALK 238
Affinity Capture-MS Homo sapiens
48 CUL4A 8451
Affinity Capture-MS Homo sapiens
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