Gene description for PHACTR4
Gene name phosphatase and actin regulator 4
Gene symbol PHACTR4
Other names/aliases PPP1R124
Species Homo sapiens
 Database cross references - PHACTR4
ExoCarta ExoCarta_65979
Vesiclepedia VP_65979
Entrez Gene 65979
HGNC 25793
MIM 608726
UniProt Q8IZ21  
 PHACTR4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for PHACTR4
Molecular Function
    actin binding GO:0003779 IBA
    actin binding GO:0003779 ISS
    protein phosphatase 1 binding GO:0008157 ISS
    protein phosphatase activator activity GO:0072542 ISS
Biological Process
    neural crest cell migration GO:0001755 IBA
    neural crest cell migration GO:0001755 ISS
    neural tube closure GO:0001843 ISS
    Rho protein signal transduction GO:0007266 ISS
    actin cytoskeleton organization GO:0030036 IBA
    actin cytoskeleton organization GO:0030036 ISS
    positive regulation of catalytic activity GO:0043085 ISS
    enteric nervous system development GO:0048484 ISS
    regulation of cell cycle GO:0051726 ISS
    closure of optic fissure GO:0061386 ISS
    negative regulation of integrin-mediated signaling pathway GO:2001045 ISS
Subcellular Localization
    cytoplasm GO:0005737 IEA
    lamellipodium GO:0030027 ISS
 Experiment description of studies that identified PHACTR4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PHACTR4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAB 7529
Affinity Capture-MS Homo sapiens
2 BAG2 9532
Affinity Capture-MS Homo sapiens
3 TOMM40 10452
Affinity Capture-MS Homo sapiens
4 LLGL2 3993
Affinity Capture-MS Homo sapiens
5 YWHAG 7532
Affinity Capture-MS Homo sapiens
6 RAB35 11021
Proximity Label-MS Homo sapiens
7 HSPA2 3306
Affinity Capture-MS Homo sapiens
8 EBAG9 9166
Proximity Label-MS Homo sapiens
9 MCAM 4162
Proximity Label-MS Homo sapiens
10 GRB2 2885
Two-hybrid Homo sapiens
11 Cd2ap 12488
Affinity Capture-MS Mus musculus
12 Naa15 74838
Affinity Capture-MS Mus musculus
13 LAMTOR1 55004
Proximity Label-MS Homo sapiens
14 MARCKS 4082
Proximity Label-MS Homo sapiens
15 RAB5A 5868
Proximity Label-MS Homo sapiens
16 GJA1 2697
Proximity Label-MS Homo sapiens
17 ACTG1 71
Affinity Capture-MS Homo sapiens
18 PARD3 56288
Proximity Label-MS Homo sapiens
19 EPB41L4A 64097
Proximity Label-MS Homo sapiens
20 LAMP1 3916
Proximity Label-MS Homo sapiens
21 PPP1CA 5499
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ACTC1 70
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
23 ARHGAP12  
Affinity Capture-MS Homo sapiens
24 GAPDHS 26330
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 LCK 3932
Proximity Label-MS Homo sapiens
26 RAB9B 51209
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
28 CALM1 801
Reconstituted Complex Homo sapiens
29 ARF6 382
Proximity Label-MS Homo sapiens
30 CXADR 1525
Proximity Label-MS Homo sapiens
31 HSPA1A 3303
Affinity Capture-MS Homo sapiens
32 STX4 6810
Proximity Label-MS Homo sapiens
33 C11orf52 91894
Proximity Label-MS Homo sapiens
34 OCLN 100506658
Proximity Label-MS Homo sapiens
35 APP 351
Reconstituted Complex Homo sapiens
36 LYN 4067
Proximity Label-MS Homo sapiens
37 DNAJC5 80331
Proximity Label-MS Homo sapiens
38 PPP1CC 5501
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 UBE2I 7329
Affinity Capture-MS Homo sapiens
40 CDH1 999
Proximity Label-MS Homo sapiens
41 YWHAZ 7534
Affinity Capture-MS Homo sapiens
42 MLLT4 4301
Proximity Label-MS Homo sapiens
43 YWHAE 7531
Affinity Capture-MS Homo sapiens
44 DYNC1LI2 1783
Proximity Label-MS Homo sapiens
45 AI314180 230249
Affinity Capture-MS Mus musculus
46 YWHAH 7533
Affinity Capture-MS Homo sapiens
47 RAB11A 8766
Proximity Label-MS Homo sapiens
48 FLOT1 10211
Proximity Label-MS Homo sapiens
49 IFIT1 3434
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 EPHA2 1969
Proximity Label-MS Homo sapiens
51 YWHAQ 10971
Affinity Capture-MS Homo sapiens
52 EZR 7430
Proximity Label-MS Homo sapiens
53 ACTB 60
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 KRAS 3845
Proximity Label-MS Homo sapiens
55 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
56 CTNNA1 1495
Proximity Label-MS Homo sapiens
57 IFIT3 3437
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 HSPA12A 259217
Affinity Capture-MS Homo sapiens
59 CAV1 857
Proximity Label-MS Homo sapiens
60 BCL7A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 RHOB 388
Proximity Label-MS Homo sapiens
62 IFIT2 3433
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 RAD21 5885
Affinity Capture-MS Homo sapiens
64 STX6 10228
Proximity Label-MS Homo sapiens
65 SNRPD3 6634
Co-fractionation Homo sapiens
66 BCL7C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PHACTR4 is involved
No pathways found





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