Gene description for PPFIA1
Gene name protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1
Gene symbol PPFIA1
Other names/aliases LIP.1
LIP1
LIPRIN
Species Homo sapiens
 Database cross references - PPFIA1
ExoCarta ExoCarta_8500
Vesiclepedia VP_8500
Entrez Gene 8500
HGNC 9245
MIM 611054
UniProt Q13136  
 PPFIA1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for PPFIA1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    cell-matrix adhesion GO:0007160 TAS
    signal transduction GO:0007165 TAS
    cortical microtubule organization GO:0043622 IMP
    synapse organization GO:0050808 IBA
    negative regulation of stress fiber assembly GO:0051497 IMP
    regulation of postsynaptic membrane neurotransmitter receptor levels GO:0099072 IEA
    negative regulation of protein localization to plasma membrane GO:1903077 IMP
Subcellular Localization
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    focal adhesion GO:0005925 IDA
    cell cortex GO:0005938 IDA
    axon GO:0030424 IEA
    protein-containing complex GO:0032991 IEA
    presynaptic active zone GO:0048786 IBA
    presynaptic active zone GO:0048786 TAS
    spine apparatus GO:0097444 IEA
    Schaffer collateral - CA1 synapse GO:0098685 IEA
 Experiment description of studies that identified PPFIA1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
10
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PPFIA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTPRF 5792
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Two-hybrid Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 GJD3  
Proximity Label-MS Homo sapiens
4 TPGS1  
Affinity Capture-MS Homo sapiens
5 CNKSR1  
Reconstituted Complex Homo sapiens
6 CCNH  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 OCLN 100506658
Proximity Label-MS Homo sapiens
8 MARCKS 4082
Proximity Label-MS Homo sapiens
9 GJA1 2697
Proximity Label-MS Homo sapiens
10 PSMD11 5717
Co-fractionation Homo sapiens
11 YWHAE 7531
Affinity Capture-MS Homo sapiens
12 EPB41L5 57669
Affinity Capture-MS Homo sapiens
13 BRCA1 672
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
14 KRT37 8688
Affinity Capture-MS Homo sapiens
15 GAS8  
Two-hybrid Homo sapiens
16 KIF7 374654
Affinity Capture-MS Homo sapiens
17 STX11 8676
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 APEX1 328
Affinity Capture-RNA Homo sapiens
19 RNH1 6050
Co-fractionation Homo sapiens
20 CAPZB 832
Affinity Capture-MS Homo sapiens
21 XRCC1 7515
Affinity Capture-MS Homo sapiens
22 DROSHA  
Affinity Capture-MS Homo sapiens
23 DYRK1A 1859
Affinity Capture-MS Homo sapiens
24 PPP2R5D 5528
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
25 CXADR 1525
Proximity Label-MS Homo sapiens
26 OSBP 5007
Co-fractionation Homo sapiens
27 PRIM1  
Co-fractionation Homo sapiens
28 BRPF3  
Affinity Capture-MS Homo sapiens
29 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 AGTPBP1  
Two-hybrid Homo sapiens
31 STX6 10228
Proximity Label-MS Homo sapiens
32 YWHAH 7533
Affinity Capture-MS Homo sapiens
33 SFN 2810
Co-fractionation Homo sapiens
34 TTC9B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 RAB35 11021
Proximity Label-MS Homo sapiens
36 MYCN  
Affinity Capture-MS Homo sapiens
37 EBAG9 9166
Proximity Label-MS Homo sapiens
38 LAMTOR1 55004
Proximity Label-MS Homo sapiens
39 GBA2  
Affinity Capture-MS Homo sapiens
40 TRNT1 51095
Cross-Linking-MS (XL-MS) Homo sapiens
41 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 Rab11fip1  
Affinity Capture-MS Mus musculus
43 PPFIA2 8499
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
44 NF2 4771
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 TOLLIP 54472
Proximity Label-MS Homo sapiens
46 ING4  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
47 CYP4X1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 CTDP1 9150
Affinity Capture-MS Homo sapiens
49 CDKN1A  
Affinity Capture-MS Homo sapiens
50 PARK2  
Affinity Capture-MS Homo sapiens
51 IL17RE  
Affinity Capture-MS Homo sapiens
52 RPS10 6204
Co-fractionation Homo sapiens
53 LYN 4067
Proximity Label-MS Homo sapiens
54 MCAM 4162
Proximity Label-MS Homo sapiens
55 Ppp2ca 19052
Affinity Capture-MS Mus musculus
56 DNAJC5 80331
Proximity Label-MS Homo sapiens
57 GIT1 28964
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
58 DIRAS3  
Proximity Label-MS Homo sapiens
59 DTNB 1838
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
60 NTRK1 4914
Affinity Capture-MS Homo sapiens
61 EPB41L4A 64097
Proximity Label-MS Homo sapiens
62 HIST1H1E 3008
Co-fractionation Homo sapiens
63 PPP2CA 5515
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 TULP3 7289
Affinity Capture-MS Homo sapiens
65 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
66 CUL2 8453
Affinity Capture-MS Homo sapiens
67 LATS2 26524
Proximity Label-MS Homo sapiens
68 CARTPT  
Affinity Capture-MS Homo sapiens
69 GPS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 Ppp2r1a 51792
Affinity Capture-MS Mus musculus
71 ACTN1 87
Co-fractionation Homo sapiens
72 PICK1  
Affinity Capture-MS Homo sapiens
73 BRF1  
Affinity Capture-MS Homo sapiens
74 WHAMMP3  
Affinity Capture-MS Homo sapiens
75 BTNL9  
Affinity Capture-MS Homo sapiens
76 C16orf70  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 PSMD13 5719
Co-fractionation Homo sapiens
78 CCDC120  
Affinity Capture-MS Homo sapiens
79 PSMD8 5714
Co-fractionation Homo sapiens
80 LAMP1 3916
Proximity Label-MS Homo sapiens
81 ERC2 26059
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
82 YWHAZ 7534
Affinity Capture-MS Homo sapiens
83 ARF6 382
Proximity Label-MS Homo sapiens
84 AURKB 9212
Affinity Capture-MS Homo sapiens
85 MAPK6  
Affinity Capture-MS Homo sapiens
86 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 GRIP2 80852
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
88 RAB9A 9367
Proximity Label-MS Homo sapiens
89 AMOT 154796
Proximity Label-MS Homo sapiens
90 RHOB 388
Proximity Label-MS Homo sapiens
91 HAT1 8520
Co-fractionation Homo sapiens
92 PPFIA1 8500
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
93 PPP2CB 5516
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 FLOT1 10211
Proximity Label-MS Homo sapiens
95 TNNT1  
Two-hybrid Homo sapiens
96 TXLNA 200081
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
97 KRT18 3875
Proximity Label-MS Homo sapiens
98 LLGL2 3993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
100 PPP4C 5531
Affinity Capture-MS Homo sapiens
101 SPTAN1 6709
Co-fractionation Homo sapiens
102 PTPRD 5789
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
103 EXOC8 149371
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 TUBG1 7283
Affinity Capture-MS Homo sapiens
105 IKBKG 8517
Co-fractionation Homo sapiens
106 LCK 3932
Proximity Label-MS Homo sapiens
107 CDK18 5129
Two-hybrid Homo sapiens
108 NCOA2 10499
Affinity Capture-Western Homo sapiens
109 CAGE1 285782
Two-hybrid Homo sapiens
110 MAGEA9  
Affinity Capture-MS Homo sapiens
111 C11orf52 91894
Proximity Label-MS Homo sapiens
112 BRK1 55845
Affinity Capture-MS Homo sapiens
113 MBIP  
Two-hybrid Homo sapiens
114 RAB2A 5862
Proximity Label-MS Homo sapiens
115 PTPRS 5802
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
Two-hybrid Homo sapiens
116 Kif4  
Affinity Capture-MS Mus musculus
117 FAM161A  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
118 RAB11A 8766
Proximity Label-MS Homo sapiens
119 SERPINB2 5055
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
120 EPHA2 1969
Proximity Label-MS Homo sapiens
121 KRAS 3845
Proximity Label-MS Homo sapiens
122 PSMD1 5707
Co-fractionation Homo sapiens
123 PSMC4 5704
Co-fractionation Homo sapiens
124 RPA3 6119
Proximity Label-MS Homo sapiens
125 CAV1 857
Proximity Label-MS Homo sapiens
126 NDN 4692
Two-hybrid Homo sapiens
127 CDC5L 988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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