Experiment description of studies that identified MICALL1 in sEVs

1 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for MICALL1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 YWHAH 7533 Affinity Capture-MS Homo sapiens 2 YWHAE 7531 Affinity Capture-MS Homo sapiens 3 YEATS4   Affinity Capture-MS Homo sapiens 4 HSPA6 3310 Co-fractionation Homo sapiens 5 RAB35 11021 Proximity Label-MS Homo sapiens 6 ZBTB21   Affinity Capture-MS Homo sapiens 7 ANKFY1 51479 Affinity Capture-Western Homo sapiens 8 ZNF785   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 9 PARP1 142 Proximity Label-MS Homo sapiens 10 PACSIN1   Affinity Capture-MS Homo sapiens 11 KSR1   Affinity Capture-MS Homo sapiens 12 MARCKS 4082 Proximity Label-MS Homo sapiens 13 LAMTOR1 55004 Proximity Label-MS Homo sapiens 14 RAB8B 51762 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 15 CAPZA2 830 Affinity Capture-MS Homo sapiens 16 YWHAB 7529 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 17 RAB15 376267 Affinity Capture-MS Homo sapiens 18 SH3GL1 6455 Affinity Capture-MS Homo sapiens 19 RAB9A 9367 Proximity Label-MS Homo sapiens 20 SF3A2 8175 Affinity Capture-MS Homo sapiens 21 MRPL9 65005 Affinity Capture-MS Homo sapiens 22 CD2AP 23607 Affinity Capture-MS Homo sapiens 23 PACSIN2 11252 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 24 CUL3 8452 Affinity Capture-MS Homo sapiens 25 YWHAZ 7534 Affinity Capture-MS Homo sapiens 26 EHD4 30844 Affinity Capture-MS Homo sapiens 27 MRPL50 54534 Affinity Capture-MS Homo sapiens 28 PACSIN3 29763 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 29 ACTN3 89 Affinity Capture-MS Homo sapiens 30 TCERG1 10915 Cross-Linking-MS (XL-MS) Homo sapiens 31 PRKACA 5566 Proximity Label-MS Homo sapiens 32 ZP3 7784 Two-hybrid Homo sapiens 33 LYN 4067 Proximity Label-MS Homo sapiens 34 MCAM 4162 Proximity Label-MS Homo sapiens 35 CNOT4 4850 Co-fractionation Homo sapiens 36 KIF13B 23303 Affinity Capture-MS Homo sapiens 37 CAPZB 832 Affinity Capture-MS Homo sapiens 38 RBM4 5936 Affinity Capture-MS Homo sapiens 39 CGN   Affinity Capture-MS Homo sapiens 40 ACTR5 79913 Affinity Capture-MS Homo sapiens 41 CDH1 999 Proximity Label-MS Homo sapiens 42 CBY1   Affinity Capture-MS Homo sapiens 43 HECW2   Affinity Capture-MS Homo sapiens 44 KLF8   Affinity Capture-MS Homo sapiens 45 PXN 5829 Co-fractionation Homo sapiens 46 RAB11A 8766 Proximity Label-MS Homo sapiens 47 C17orf70   Affinity Capture-MS Homo sapiens 48 EPHA2 1969 Affinity Capture-MS Homo sapiens 49 BICD2 23299 Proximity Label-MS Homo sapiens 50 KRAS 3845 Proximity Label-MS Homo sapiens 51 SH3KBP1 30011 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 52 YWHAG 7532 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 53 EHD3 30845 Affinity Capture-MS Homo sapiens 54 PLCG1 5335 Affinity Capture-MS Homo sapiens 55 KCTD3   Affinity Capture-MS Homo sapiens 56 CAV1 857 Proximity Label-MS Homo sapiens 57 RHOB 388 Proximity Label-MS Homo sapiens 58 STX6 10228 Proximity Label-MS Homo sapiens 59 EHD1 10938 Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens View the network

Pathways in which MICALL1 is involved

No pathways found