Gene description for HERC3
Gene name HECT and RLD domain containing E3 ubiquitin protein ligase 3
Gene symbol HERC3
Other names/aliases -
Species Homo sapiens
 Database cross references - HERC3
ExoCarta ExoCarta_8916
Vesiclepedia VP_8916
Entrez Gene 8916
HGNC 4876
MIM 605200
UniProt Q15034  
 HERC3 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for HERC3
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin protein ligase activity GO:0061630 IBA
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    protein ubiquitination GO:0016567 IBA
    protein ubiquitination GO:0016567 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasmic vesicle GO:0031410 IEA
 Experiment description of studies that identified HERC3 in sEVs
1
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for HERC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TTLL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 POTEC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 RELA 5970
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 NME2 4831
Two-hybrid Homo sapiens
5 FAM189B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 ISG15 9636
Affinity Capture-MS Homo sapiens
7 CCL22 6367
Two-hybrid Homo sapiens
8 FBXW11  
Affinity Capture-MS Homo sapiens
9 TK1 7083
Two-hybrid Homo sapiens
10 RXFP1  
Affinity Capture-MS Homo sapiens
11 GDA 9615
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 KIF20A 10112
Affinity Capture-MS Homo sapiens
13 CYP8B1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 HIST2H3PS2 440686
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 UBQLN2 29978
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
16 KCNC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 NUDCD2 134492
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 PSMA4 5685
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
19 EIF5A2 56648
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
20 HSD17B3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 OR6M1  
Affinity Capture-MS Homo sapiens
22 UBC 7316
Reconstituted Complex Homo sapiens
23 CD33 945
Two-hybrid Homo sapiens
24 SLC25A2  
Affinity Capture-MS Homo sapiens
25 PSMD4 5710
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
26 SHMT2 6472
Affinity Capture-RNA Homo sapiens
27 MOGAT2  
Affinity Capture-MS Homo sapiens
28 RCBTB1  
Affinity Capture-MS Homo sapiens
29 NXF1 10482
Affinity Capture-RNA Homo sapiens
30 RHBDL1  
Affinity Capture-MS Homo sapiens
31 LPCAT2 54947
Affinity Capture-MS Homo sapiens
32 RDH5 5959
Affinity Capture-MS Homo sapiens
33 ARHGAP25  
Affinity Capture-MS Homo sapiens
34 MOV10 4343
Affinity Capture-RNA Homo sapiens
35 HERC6  
Affinity Capture-Western Homo sapiens
36 AWAT2  
Affinity Capture-MS Homo sapiens
37 UBE2L3 7332
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
38 DIO3  
Affinity Capture-MS Homo sapiens
39 KCNA6  
Affinity Capture-MS Homo sapiens
40 DSG4 147409
Affinity Capture-MS Homo sapiens
41 ACOX3 8310
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 FAM189A2  
Affinity Capture-MS Homo sapiens
43 PSMC5 5705
Affinity Capture-MS Homo sapiens
44 CYP39A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 IL17RC 84818
Affinity Capture-MS Homo sapiens
46 PSMC4 5704
Affinity Capture-MS Homo sapiens
47 PSMC2 5701
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
48 UBQLN1 29979
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
49 PSMD2 5708
Affinity Capture-MS Homo sapiens
50 UBE2D1 7321
Reconstituted Complex Homo sapiens
51 DCST1  
Affinity Capture-MS Homo sapiens
52 HERC2 8924
Affinity Capture-MS Homo sapiens
53 TMTC4  
Affinity Capture-MS Homo sapiens
54 TMEM62  
Affinity Capture-MS Homo sapiens
55 SMAD7  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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