Gene description for RASGRP2
Gene name RAS guanyl releasing protein 2 (calcium and DAG-regulated)
Gene symbol RASGRP2
Other names/aliases CALDAG-GEFI
CDC25L
Species Homo sapiens
 Database cross references - RASGRP2
ExoCarta ExoCarta_10235
Vesiclepedia VP_10235
Entrez Gene 10235
HGNC 9879
MIM 605577
UniProt Q7LDG7  
 RASGRP2 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for RASGRP2
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    calcium ion binding GO:0005509 NAS
    calcium ion binding GO:0005509 TAS
    lipid binding GO:0008289 TAS
    diacylglycerol binding GO:0019992 NAS
Biological Process
    regulation of cell growth GO:0001558 NAS
    signal transduction GO:0007165 TAS
    Ras protein signal transduction GO:0007265 IBA
    Ras protein signal transduction GO:0007265 NAS
    positive regulation of GTPase activity GO:0043547 IDA
    cellular response to calcium ion GO:0071277 IDA
Subcellular Localization
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    ruffle membrane GO:0032587 IEA
    neuron projection GO:0043005 IEA
    synapse GO:0045202 IEA
 Experiment description of studies that identified RASGRP2 in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RASGRP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTCHD2  
Two-hybrid Homo sapiens
2 APP 351
Reconstituted Complex Homo sapiens
3 ALAD 210
Affinity Capture-MS Homo sapiens
4 NHLRC2 374354
Affinity Capture-MS Homo sapiens
5 CEBPA  
Protein-peptide Homo sapiens
6 DDX58 23586
Affinity Capture-RNA Homo sapiens
View the network image/svg+xml



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