Gene description for MTHFS
Gene name 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase)
Gene symbol MTHFS
Other names/aliases HsT19268
Species Homo sapiens
 Database cross references - MTHFS
ExoCarta ExoCarta_10588
Vesiclepedia VP_10588
Entrez Gene 10588
HGNC 7437
MIM 604197
UniProt P49914  
 MTHFS identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MTHFS
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    folic acid binding GO:0005542 IDA
    5-formyltetrahydrofolate cyclo-ligase activity GO:0030272 IBA
    5-formyltetrahydrofolate cyclo-ligase activity GO:0030272 IDA
    5-formyltetrahydrofolate cyclo-ligase activity GO:0030272 IMP
    5-formyltetrahydrofolate cyclo-ligase activity GO:0030272 NAS
    5-formyltetrahydrofolate cyclo-ligase activity GO:0030272 TAS
    metal ion binding GO:0046872 IEA
Biological Process
    glutamate metabolic process GO:0006536 IMP
    folic acid-containing compound biosynthetic process GO:0009396 IBA
    formate metabolic process GO:0015942 NAS
    tetrahydrofolate interconversion GO:0035999 IBA
    tetrahydrofolate interconversion GO:0035999 IMP
    tetrahydrofolate metabolic process GO:0046653 IDA
    folic acid metabolic process GO:0046655 TAS
    folic acid catabolic process GO:0046657 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrial matrix GO:0005759 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified MTHFS in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MTHFS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DYNC1H1 1778
Cross-Linking-MS (XL-MS) Homo sapiens
2 RNF43  
Proximity Label-MS Homo sapiens
3 PLCL2 23228
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 TSC22D4 81628
Two-hybrid Homo sapiens
5 NDUFS3 4722
Affinity Capture-MS Homo sapiens
6 ACTA2 59
Affinity Capture-MS Homo sapiens
7 RUVBL2 10856
Cross-Linking-MS (XL-MS) Homo sapiens
8 RGS4  
Affinity Capture-MS Homo sapiens
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