Gene description for Chd4
Gene name chromodomain helicase DNA binding protein 4
Gene symbol Chd4
Other names/aliases 9530019N15Rik
AA617397
BC005710
D6Ertd380e
Mi-2beta
mKIAA4075
Species Mus musculus
 Database cross references - Chd4
ExoCarta ExoCarta_107932
Vesiclepedia VP_107932
Entrez Gene 107932
UniProt Q6PDQ2  
 Chd4 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Chd4
Molecular Function
    DNA binding GO:0003677 IEA
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 ISO
    nucleosomal DNA binding GO:0031492 ISO
    nucleic acid binding GO:0003676 IEA
    transcription factor binding GO:0008134 IPI
    ATP binding GO:0005524 IEA
    microtubule binding GO:0008017 IEA
    helicase activity GO:0004386 IEA
    RNA polymerase II distal enhancer sequence-specific DNA binding GO:0000980 ISO
    hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides GO:0016818 IEA
    hydrolase activity GO:0016787 IEA
    RNA polymerase II repressing transcription factor binding GO:0001103 ISO
    zinc ion binding GO:0008270 IEA
    ATP-dependent helicase activity GO:0008026 IEA
Biological Process
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 ISO
    chromatin modification GO:0016568 IEA
    spindle assembly GO:0051225 IEA
    regulation of transcription, DNA-templated GO:0006355 IEA
    ATP-dependent chromatin remodeling GO:0043044 ISO
    chromatin organization GO:0006325 IEA
    terminal button organization GO:0072553 ISO
    transcription, DNA-templated GO:0006351 IEA
Subcellular Localization
    cytoskeleton GO:0005856 IEA
    protein complex GO:0043234 ISO
    membrane GO:0016020 ISO
    nuclear chromatin GO:0000790 ISO
    nucleoplasm GO:0005654 ISO
    nucleus GO:0005634 ISO
    NuRD complex GO:0016581 ISO
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 ISO
 Experiment description of studies that identified Chd4 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Chd4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Chd4 is involved
PathwayEvidenceSource
HDACs deacetylate histones IEA Reactome
RNA Polymerase I Transcription Initiation IEA Reactome





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