Gene description for CD300A
Gene name CD300a molecule
Gene symbol CD300A
Other names/aliases CLM-8
CMRF-35-H9
CMRF-35H
CMRF35-H
CMRF35-H9
CMRF35H
CMRF35H9
IGSF12
IRC1
IRC1/IRC2
IRC2
IRp60
Species Homo sapiens
 Database cross references - CD300A
ExoCarta ExoCarta_11314
Vesiclepedia VP_11314
Entrez Gene 11314
HGNC 19319
MIM 606790
UniProt Q9UGN4  
 CD300A identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
 Gene ontology annotations for CD300A
Molecular Function
    phosphatidylserine binding GO:0001786 IDA
    transmembrane signaling receptor activity GO:0004888 IBA
    protein binding GO:0005515 IPI
    phosphatidylethanolamine binding GO:0008429 IDA
    signaling receptor activity GO:0038023 IDA
Biological Process
    immune system process GO:0002376 IEA
    cell adhesion GO:0007155 IEA
    signal transduction GO:0007165 IBA
    negative regulation of B cell proliferation GO:0030889 IMP
    negative regulation of mast cell activation involved in immune response GO:0033007 IDA
    negative regulation of MyD88-dependent toll-like receptor signaling pathway GO:0034125 IMP
    intracellular signal transduction GO:0035556 IDA
    negative regulation of mast cell degranulation GO:0043305 IDA
    negative regulation of MAP kinase activity GO:0043407 IDA
    regulation of innate immune response GO:0045088 IBA
    negative regulation of fibroblast proliferation GO:0048147 IDA
    regulation of T cell receptor signaling pathway GO:0050856 IDA
    negative regulation of B cell receptor signaling pathway GO:0050859 IDA
    negative regulation of NK T cell activation GO:0051134 IDA
    negative regulation of phagocytosis, engulfment GO:0060101 IMP
    negative regulation of neutrophil activation GO:1902564 IDA
    negative regulation of eosinophil activation GO:1902567 IDA
    negative regulation of eosinophil migration GO:2000417 IDA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 IDA
    extracellular exosome GO:0070062 HDA
    tertiary granule membrane GO:0070821 TAS
    ficolin-1-rich granule membrane GO:0101003 TAS
 Experiment description of studies that identified CD300A in sEVs
1
Experiment ID 79
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
 Protein-protein interactions for CD300A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TMEM60  
Two-hybrid Homo sapiens
2 TMEM199  
Two-hybrid Homo sapiens
3 KRT38 8687
Two-hybrid Homo sapiens
4 TMEM218  
Two-hybrid Homo sapiens
5 KIF14 9928
Affinity Capture-MS Homo sapiens
6 KRTAP10-3  
Two-hybrid Homo sapiens
7 SEC22A  
Two-hybrid Homo sapiens
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