Gene description for CYP2U1
Gene name cytochrome P450, family 2, subfamily U, polypeptide 1
Gene symbol CYP2U1
Other names/aliases P450TEC
SPG49
SPG56
Species Homo sapiens
 Database cross references - CYP2U1
ExoCarta ExoCarta_113612
Vesiclepedia VP_113612
Entrez Gene 113612
HGNC 20582
MIM 610670
UniProt Q7Z449  
 CYP2U1 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CYP2U1
Molecular Function
    monooxygenase activity GO:0004497 TAS
    iron ion binding GO:0005506 IEA
    steroid hydroxylase activity GO:0008395 IBA
    oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen GO:0016712 IBA
    heme binding GO:0020037 IBA
    arachidonate omega-hydroxylase activity GO:0052869 IDA
    long-chain fatty acid omega-hydroxylase activity GO:0102033 IEA
Biological Process
    organic acid metabolic process GO:0006082 IBA
    xenobiotic metabolic process GO:0006805 IBA
    omega-hydroxylase P450 pathway GO:0097267 IDA
    omega-hydroxylase P450 pathway GO:0097267 TAS
    cytochrome metabolic process GO:1903604 TAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    mitochondrial inner membrane GO:0005743 IEA
    endoplasmic reticulum membrane GO:0005789 TAS
    intracellular membrane-bounded organelle GO:0043231 IBA
 Experiment description of studies that identified CYP2U1 in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CYP2U1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LGR4 55366
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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