Gene description for Mapk8
Gene name mitogen-activated protein kinase 8
Gene symbol Mapk8
Other names/aliases JNK
Species Rattus norvegicus
 Database cross references - Mapk8
ExoCarta ExoCarta_116554
Entrez Gene 116554
UniProt P49185  
 Mapk8 identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Mapk8
Molecular Function
    kinase activity GO:0016301 ISO
    histone deacetylase binding GO:0042826 ISO
    ATP binding GO:0005524 IEA
    enzyme binding GO:0019899 ISO
    protein serine/threonine kinase activity GO:0004674 ISO
    histone deacetylase regulator activity GO:0035033 ISO
    protein binding GO:0005515 IPI
    JUN kinase activity GO:0004705 TAS
Biological Process
    peptidyl-serine phosphorylation GO:0018105 ISO
    response to heat GO:0009408 IDA
    JNK cascade GO:0007254 ISS
    cellular response to mechanical stimulus GO:0071260 ISO
    response to UV GO:0009411 ISO
    inflammatory response GO:0006954 IDA
    regulation of protein localization GO:0032880 ISO
    positive regulation of gene expression GO:0010628 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    regulation of circadian rhythm GO:0042752 ISS
    negative regulation of protein binding GO:0032091 ISO
    neuronal stem cell maintenance GO:0097150 IMP
    positive regulation of protein metabolic process GO:0051247 ISO
    cellular response to hydrogen peroxide GO:0070301 ISO
    ossification GO:0001503 ISO
    JUN phosphorylation GO:0007258 TAS
    positive regulation of determination of dorsal identity GO:2000017 ISO
    signal transduction GO:0007165 IDA
    protein phosphorylation GO:0006468 ISO
    response to osmotic stress GO:0006970 IDA
    response to cadmium ion GO:0046686 ISO
    response to steroid hormone GO:0048545 IEP
    positive regulation of cyclase activity GO:0031281 ISO
    cellular response to nitric oxide GO:0071732 ISO
    type B pancreatic cell apoptotic process GO:0097050 IMP
    response to hydrogen peroxide GO:0042542 IDA
    peptidyl-threonine phosphorylation GO:0018107 ISO
    cellular response to lipopolysaccharide GO:0071222 ISO
    programmed necrotic cell death GO:0097300 ISO
    neuron projection development GO:0031175 IMP
    rhythmic process GO:0048511 IEA
    positive regulation of apoptotic process GO:0043065 IEP
    regulation of transcription, DNA-templated GO:0006355 IDA
    positive regulation of DNA replication GO:0045740 IMP
    regulation of gene expression GO:0010468 ISO
    positive regulation of apoptotic signaling pathway GO:2001235 ISO
    positive regulation of microtubule polymerization GO:0031116 IMP
    positive regulation of deacetylase activity GO:0090045 ISO
    positive regulation of cell migration GO:0030335 IDA
    regulation of histone deacetylation GO:0031063 ISO
Subcellular Localization
    mitochondrion GO:0005739 ISO
    nucleus GO:0005634 ISO
    cytosol GO:0005829 TAS
    perikaryon GO:0043204 IDA
    axon GO:0030424 IDA
    cytoplasm GO:0005737 ISO
    dendrite cytoplasm GO:0032839 IDA
    vesicle GO:0031982 IDA
 Experiment description of studies that identified Mapk8 in exosomes
1
Experiment ID 92
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Mapk8
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Mapk8 is involved
PathwayEvidenceSource
NRAGE signals death through JNK IEA Reactome





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