Gene description for Capn1
Gene name calpain 1
Gene symbol Capn1
Other names/aliases Capa-1
Capa1
mu-calpin
Species Mus musculus
 Database cross references - Capn1
ExoCarta ExoCarta_12333
Vesiclepedia VP_12333
Entrez Gene 12333
UniProt O35350  
 Capn1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Capn1
Molecular Function
    cysteine-type peptidase activity GO:0008234 IEA
    calcium-dependent cysteine-type endopeptidase activity GO:0004198 TAS
    protein binding GO:0005515 IPI
    calcium ion binding GO:0005509 ISO
    peptidase activity GO:0008233 IEA
    cytoskeletal protein binding GO:0008092 ISO
    hydrolase activity GO:0016787 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    proteolysis GO:0006508 ISO
    receptor catabolic process GO:0032801 IDA
    mammary gland involution GO:0060056 IMP
    protein autoprocessing GO:0016540 ISO
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 IDA
    plasma membrane GO:0005886 ISO
    intracellular GO:0005622 IEA
    focal adhesion GO:0005925 ISO
    lysosome GO:0005764 IDA
    membrane GO:0016020 ISO
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Capn1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Capn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Capn1 is involved
PathwayEvidenceSource
Degradation of the extracellular matrix IEA Reactome





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