Gene description for Col3a1
Gene name collagen, type III, alpha 1
Gene symbol Col3a1
Other names/aliases AW550625
Col3a-1
MMS10-W
Ms10w
mKIAA4231
Species Mus musculus
 Database cross references - Col3a1
ExoCarta ExoCarta_12825
Vesiclepedia VP_12825
Entrez Gene 12825
UniProt P08121  
 Col3a1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Col3a1
Molecular Function
    protease binding GO:0002020 IEA
    protease binding GO:0002020 ISO
    integrin binding GO:0005178 IEA
    integrin binding GO:0005178 ISO
    extracellular matrix structural constituent GO:0005201 ISO
    extracellular matrix structural constituent conferring tensile strength GO:0030020 IBA
    extracellular matrix structural constituent conferring tensile strength GO:0030020 RCA
    SMAD binding GO:0046332 IPI
    metal ion binding GO:0046872 IEA
    platelet-derived growth factor binding GO:0048407 ISO
Biological Process
    blood vessel development GO:0001568 IMP
    in utero embryonic development GO:0001701 IMP
    neuron migration GO:0001764 IMP
    tissue homeostasis GO:0001894 IMP
    chondrocyte differentiation GO:0002062 IMP
    cell-matrix adhesion GO:0007160 IEA
    cell-matrix adhesion GO:0007160 ISO
    transforming growth factor beta receptor signaling pathway GO:0007179 IEA
    transforming growth factor beta receptor signaling pathway GO:0007179 ISO
    integrin-mediated signaling pathway GO:0007229 IEA
    integrin-mediated signaling pathway GO:0007229 ISO
    heart development GO:0007507 IEA
    heart development GO:0007507 ISO
    response to radiation GO:0009314 IEA
    response to radiation GO:0009314 ISO
    response to mechanical stimulus GO:0009612 IEA
    gene expression GO:0010467 IMP
    peptide cross-linking GO:0018149 ISO
    layer formation in cerebral cortex GO:0021819 IMP
    cerebral cortex development GO:0021987 IMP
    extracellular matrix organization GO:0030198 IBA
    extracellular matrix organization GO:0030198 IMP
    collagen fibril organization GO:0030199 IMP
    collagen fibril organization GO:0030199 IMP
    collagen fibril organization GO:0030199 ISO
    lung development GO:0030324 IMP
    transforming growth factor beta1 production GO:0032905 IMP
    response to cytokine GO:0034097 IEA
    response to cytokine GO:0034097 ISO
    positive regulation of Rho protein signal transduction GO:0035025 IDA
    multicellular organism growth GO:0035264 IMP
    aorta development GO:0035904 IMP
    limb joint morphogenesis GO:0036022 IMP
    wound healing GO:0042060 IEA
    wound healing GO:0042060 ISO
    skin development GO:0043588 IMP
    skin development GO:0043588 ISO
    fibroblast proliferation GO:0048144 IMP
    elastic fiber assembly GO:0048251 IMP
    digestive tract development GO:0048565 IMP
    digestive tract development GO:0048565 IMP
    negative regulation of immune response GO:0050777 IEA
    negative regulation of immune response GO:0050777 ISO
    cartilage development GO:0051216 IMP
    endochondral bone morphogenesis GO:0060350 IMP
    aorta smooth muscle tissue morphogenesis GO:0060414 IMP
    cellular response to amino acid stimulus GO:0071230 IDA
    basement membrane organization GO:0071711 IMP
    supramolecular fiber organization GO:0097435 ISO
    response to angiotensin GO:1990776 IMP
    negative regulation of neuron migration GO:2001223 IMP
Subcellular Localization
    collagen trimer GO:0005581 IDA
    collagen type III trimer GO:0005586 IDA
    collagen type III trimer GO:0005586 ISO
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 ISO
    extracellular matrix GO:0031012 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IBA
    collagen-containing extracellular matrix GO:0062023 ISO
 Experiment description of studies that identified Col3a1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Col3a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Plxnd1  
Co-fractionation Mus musculus
2 Smad4 17128
Affinity Capture-Western Mus musculus
3 Serpinf1 20317
Reconstituted Complex Mus musculus
4 Smad3  
Affinity Capture-Western Mus musculus
5 Smad7  
Affinity Capture-Western Mus musculus
6 Smad2  
Affinity Capture-Western Mus musculus
7 Tfap2c  
Co-fractionation Mus musculus
8 Smad1  
Affinity Capture-Western Mus musculus
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