Gene description for Smad4
Gene name SMAD family member 4
Gene symbol Smad4
Other names/aliases AW743858
D18Wsu70e
DPC4
Madh4
Species Mus musculus
 Database cross references - Smad4
ExoCarta ExoCarta_17128
Entrez Gene 17128
UniProt P97471  
 Smad4 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Smad4
Molecular Function
    protein heterodimerization activity GO:0046982 IDA
    transcription regulatory region DNA binding GO:0044212 ISO
    identical protein binding GO:0042802 ISO
    RNA polymerase II regulatory region sequence-specific DNA binding GO:0000977 IDA
    RNA polymerase II transcription factor binding GO:0001085 IPI
    sequence-specific DNA binding GO:0043565 ISO
    metal ion binding GO:0046872 IEA
    protein homodimerization activity GO:0042803 ISO
    protein binding GO:0005515 IPI
    sequence-specific DNA binding transcription factor activity GO:0003700 ISO
    transforming growth factor beta receptor, common-partner cytoplasmic mediator activity GO:0030616 ISO
    RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001077 ISO
    DNA binding GO:0003677 ISO
    RNA polymerase II transcription factor binding transcription factor activity GO:0001076 IDA
    I-SMAD binding GO:0070411 ISO
    collagen binding GO:0005518 IPI
    RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001228 IDA
    chromatin binding GO:0003682 IDA
    core promoter proximal region sequence-specific DNA binding GO:0000987 ISO
    protein binding transcription factor activity GO:0000988 ISO
    filamin binding GO:0031005 ISO
    SMAD binding GO:0046332 ISO
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 ISO
    R-SMAD binding GO:0070412 ISO
Biological Process
    developmental growth GO:0048589 IMP
    transcription, DNA-templated GO:0006351 IEA
    positive regulation of transforming growth factor beta receptor signaling pathway GO:0030511 ISO
    tissue morphogenesis GO:0048729 IMP
    in utero embryonic development GO:0001701 IMP
    cell proliferation GO:0008283 IMP
    response to transforming growth factor beta GO:0071559 ISO
    regulation of transforming growth factor beta receptor signaling pathway GO:0017015 ISO
    cardiac septum development GO:0003279 IGI
    transcription from RNA polymerase II promoter GO:0006366 ISO
    positive regulation of SMAD protein import into nucleus GO:0060391 IMP
    negative regulation of cell death GO:0060548 IMP
    kidney development GO:0001822 IMP
    sebaceous gland development GO:0048733 IMP
    SMAD protein signal transduction GO:0060395 ISO
    regulation of cell proliferation GO:0042127 IMP
    anterior/posterior pattern specification GO:0009952 IMP
    SMAD protein complex assembly GO:0007183 ISO
    regulation of transforming growth factor beta2 production GO:0032909 ISO
    regulation of hair follicle development GO:0051797 IMP
    negative regulation of transcription, DNA-templated GO:0045892 ISO
    positive regulation of cell proliferation involved in heart valve morphogenesis GO:0003251 IMP
    transforming growth factor beta receptor signaling pathway GO:0007179 ISO
    regulation of transcription from RNA polymerase II promoter GO:0006357 IDA
    positive regulation of epithelial to mesenchymal transition GO:0010718 IMP
    formation of anatomical boundary GO:0048859 IMP
    nephrogenic mesenchyme morphogenesis GO:0072134 IMP
    neural crest cell differentiation GO:0014033 IMP
    atrioventricular valve formation GO:0003190 IGI
    gastrulation GO:0007369 IMP
    BMP signaling pathway GO:0030509 ISO
    regulation of binding GO:0051098 IDA
    negative regulation of protein catabolic process GO:0042177 ISO
    gastrulation with mouth forming second GO:0001702 IMP
    axon guidance GO:0007411 IMP
    brainstem development GO:0003360 IMP
    intracellular signal transduction GO:0035556 ISO
    endocardial cell differentiation GO:0060956 IMP
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    epithelial to mesenchymal transition involved in endocardial cushion formation GO:0003198 IMP
    endothelial cell activation GO:0042118 IMP
    endoderm development GO:0007492 IMP
    negative regulation of cell proliferation GO:0008285 IGI
    palate development GO:0060021 IMP
    positive regulation of BMP signaling pathway GO:0030513 ISO
    metanephric mesenchyme morphogenesis GO:0072133 IMP
    neuron fate commitment GO:0048663 IMP
    positive regulation of pathway-restricted SMAD protein phosphorylation GO:0010862 IMP
    branching involved in ureteric bud morphogenesis GO:0001658 IGI
    regulation of transcription, DNA-templated GO:0006355 IEA
    response to hypoxia GO:0001666 ISO
    mesoderm development GO:0007498 IMP
    negative regulation of cell growth GO:0030308 ISO
    positive regulation of transcription, DNA-templated GO:0045893 ISO
    atrioventricular canal development GO:0036302 IGI
    somite rostral/caudal axis specification GO:0032525 IMP
Subcellular Localization
    nucleus GO:0005634 ISO
    protein complex GO:0043234 ISO
    intracellular GO:0005622 IEA
    nucleoplasm GO:0005654 ISO
    RNA polymerase II transcription factor complex GO:0090575 IDA
    SMAD protein complex GO:0071141 ISO
    nuclear chromatin GO:0000790 ISO
    transcription factor complex GO:0005667 ISO
    cytoplasm GO:0005737 ISO
    activin responsive factor complex GO:0032444 ISO
    centrosome GO:0005813 ISO
 Experiment description of studies that identified Smad4 in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Smad4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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