Gene description for Serpinf1
Gene name serine (or cysteine) peptidase inhibitor, clade F, member 1
Gene symbol Serpinf1
Other names/aliases AI195227
EPC-1
Pedf
Pedfl
Sdf3
Species Mus musculus
 Database cross references - Serpinf1
ExoCarta ExoCarta_20317
Vesiclepedia VP_20317
Entrez Gene 20317
UniProt P97298  
 Serpinf1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Serpinf1
Molecular Function
    serine-type endopeptidase inhibitor activity GO:0004867 IBA
    serine-type endopeptidase inhibitor activity GO:0004867 IEA
Biological Process
    kidney development GO:0001822 IEA
    short-term memory GO:0007614 IEA
    short-term memory GO:0007614 ISO
    response to acidic pH GO:0010447 IEA
    negative regulation of endothelial cell migration GO:0010596 IEA
    negative regulation of endothelial cell migration GO:0010596 ISO
    negative regulation of gene expression GO:0010629 IEA
    negative regulation of gene expression GO:0010629 ISO
    negative regulation of endopeptidase activity GO:0010951 IBA
    positive regulation of neuron projection development GO:0010976 IEA
    positive regulation of neuron projection development GO:0010976 ISO
    response to organic cyclic compound GO:0014070 ISO
    negative regulation of angiogenesis GO:0016525 IBA
    negative regulation of angiogenesis GO:0016525 IDA
    negative regulation of angiogenesis GO:0016525 ISO
    ovulation cycle GO:0042698 IEA
    response to arsenic-containing substance GO:0046685 IEA
    positive regulation of neurogenesis GO:0050769 ISO
    positive regulation of neurogenesis GO:0050769 ISS
    retina development in camera-type eye GO:0060041 IEA
    epithelial cell proliferation involved in prostate gland development GO:0060767 IMP
    negative regulation of epithelial cell proliferation involved in prostate gland development GO:0060770 IMP
    cellular response to cobalt ion GO:0071279 IEA
    cellular response to retinoic acid GO:0071300 IEA
    cellular response to glucose stimulus GO:0071333 IEA
    cellular response to dexamethasone stimulus GO:0071549 IEA
    response to peptide GO:1901652 IEA
    response to peptide GO:1901652 ISO
Subcellular Localization
    extracellular region GO:0005576 IDA
    extracellular region GO:0005576 ISO
    basement membrane GO:0005604 IEA
    basement membrane GO:0005604 ISO
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    axon GO:0030424 ISO
    melanosome GO:0042470 IEA
    neuronal cell body GO:0043025 ISO
    axon hillock GO:0043203 IEA
    axon hillock GO:0043203 ISO
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Serpinf1 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 39
MISEV standards
IEM
Biophysical techniques
TSG101|HSC70|FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Serpinf1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Zer1  
Co-fractionation Mus musculus
2 Col1a1 12842
Reconstituted Complex Mus musculus
3 Col3a1 12825
Reconstituted Complex Mus musculus
4 Cuta  
Co-fractionation Mus musculus
5 Nt5c3b  
Co-fractionation Mus musculus
6 Selenbp1  
Co-fractionation Mus musculus
7 Cab39l  
Co-fractionation Mus musculus
8 Vps51  
Co-fractionation Mus musculus
9 Tagln  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Serpinf1 is involved
No pathways found





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