Gene description for Col1a2
Gene name collagen, type I, alpha 2
Gene symbol Col1a2
Other names/aliases AA960264
AI325291
Col1a-2
Cola-2
Cola2
oim
Species Mus musculus
 Database cross references - Col1a2
ExoCarta ExoCarta_12843
Entrez Gene 12843
UniProt Q01149  
 Col1a2 identified in exosomes derived from the following tissue/cell type
Embryonic fibroblasts 18494037    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Col1a2
Molecular Function
    protein binding, bridging GO:0030674 ISO
    extracellular matrix structural constituent GO:0005201 IEA
    identical protein binding GO:0042802 ISO
    platelet-derived growth factor binding GO:0048407 ISO
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    SMAD binding GO:0046332 IPI
Biological Process
    blood vessel development GO:0001568 ISO
    transforming growth factor beta receptor signaling pathway GO:0007179 ISO
    cellular response to amino acid stimulus GO:0071230 IDA
    skeletal system development GO:0001501 ISO
    protein heterotrimerization GO:0070208 IDA
    Rho protein signal transduction GO:0007266 ISO
    regulation of blood pressure GO:0008217 ISO
    collagen fibril organization GO:0030199 ISO
    skin morphogenesis GO:0043589 ISO
Subcellular Localization
    proteinaceous extracellular matrix GO:0005578 IEA
    extracellular region GO:0005576 IEA
    extracellular space GO:0005615 ISO
    intracellular GO:0005622 ISO
    collagen type I trimer GO:0005584 ISO
    extracellular matrix GO:0031012 ISO
    extracellular exosome GO:0070062 ISO
    collagen trimer GO:0005581 IDA
 Experiment description of studies that identified Col1a2 in exosomes
1
Experiment ID 73
ISEV standards
EM
EV Biophysical techniques
HSP70|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
2
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Col1a2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here