Gene description for Diap1
Gene name diaphanous homolog 1 (Drosophila)
Gene symbol Diap1
Other names/aliases D18Wsu154e
Dia1
Diaph1
Drf1
p140mDia
Species Mus musculus
 Database cross references - Diap1
ExoCarta ExoCarta_13367
Entrez Gene 13367
UniProt O08808  
 Diap1 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Diap1
Molecular Function
    actin binding GO:0003779 IDA
    Rho GTPase binding GO:0017048 IDA
    ion channel binding GO:0044325 ISO
    poly(A) RNA binding GO:0044822 ISO
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    profilin binding GO:0005522 IDA
Biological Process
    cytoskeleton organization GO:0007010 ISO
    actin filament polymerization GO:0030041 IDA
    positive regulation of cell migration GO:0030335 ISO
    protein localization to microtubule GO:0035372 ISO
    sensory perception of sound GO:0007605 IEA
    regulation of cell shape GO:0008360 ISO
    regulation of microtubule-based process GO:0032886 ISO
    actin cytoskeleton organization GO:0030036 IDA
    cellular response to histamine GO:0071420 ISO
    actin filament organization GO:0007015 IEA
    regulation of release of sequestered calcium ion into cytosol GO:0051279 ISO
    cellular component organization GO:0016043 IEA
    neuron projection development GO:0031175 IDA
Subcellular Localization
    cell projection GO:0042995 IEA
    neuron projection GO:0043005 IDA
    cytoplasm GO:0005737 IEA
    plasma membrane GO:0005886 IEA
    ruffle membrane GO:0032587 IDA
    brush border GO:0005903 IDA
    mitotic spindle GO:0072686 ISO
    membrane GO:0016020 IEA
    cytoskeleton GO:0005856 IEA
 Experiment description of studies that identified Diap1 in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Diap1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RHOA 387
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which Diap1 is involved
PathwayEvidenceSource
RHO GTPases Activate Formins IEA Reactome





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