Gene description for Fmnl1
Gene name formin-like 1
Gene symbol Fmnl1
Other names/aliases 8030453N10Rik
AI553564
Fmnl
Fnrl
Frls
Species Mus musculus
 Database cross references - Fmnl1
ExoCarta ExoCarta_57778
Entrez Gene 57778
UniProt Q9JL26  
 Fmnl1 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Fmnl1
Molecular Function
    Rho GTPase binding GO:0017048 IEA
    GTPase activating protein binding GO:0032794 IDA
    profilin binding GO:0005522 IDA
    actin filament binding GO:0051015 IDA
    actin binding GO:0003779 IEA
    Rac GTPase binding GO:0048365 IDA
Biological Process
    cellular process GO:0009987 IMP
    regulation of cell shape GO:0008360 IEA
    actin filament severing GO:0051014 IDA
    actin cytoskeleton organization GO:0030036 IEA
    cytoskeleton organization GO:0007010 IEA
    cellular component organization GO:0016043 IEA
    substrate-dependent cell migration GO:0006929 IMP
Subcellular Localization
    cytosol GO:0005829 IDA
    membrane GO:0016020 IEA
    phagocytic vesicle GO:0045335 IDA
    cytoplasm GO:0005737 IEA
    plasma membrane GO:0005886 IDA
    cytoplasmic vesicle GO:0031410 IEA
 Experiment description of studies that identified Fmnl1 in exosomes
1
Experiment ID 214
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Fmnl1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Fmnl1 is involved
PathwayEvidenceSource
RHO GTPases Activate Formins IEA Reactome





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