Gene description for Fn1
Gene name fibronectin 1
Gene symbol Fn1
Other names/aliases E330027I09
Fn
Fn-1
Species Mus musculus
 Database cross references - Fn1
ExoCarta ExoCarta_14268
Vesiclepedia VP_14268
Entrez Gene 14268
UniProt P11276  
 Fn1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Fn1
Molecular Function
    protease binding GO:0002020 IEA
    protease binding GO:0002020 ISO
    signaling receptor binding GO:0005102 IDA
    signaling receptor binding GO:0005102 ISO
    integrin binding GO:0005178 IBA
    integrin binding GO:0005178 IEA
    integrin binding GO:0005178 ISO
    extracellular matrix structural constituent GO:0005201 RCA
    protein binding GO:0005515 IPI
    heparin binding GO:0008201 IEA
    peptidase activator activity GO:0016504 IDA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    proteoglycan binding GO:0043394 IBA
    proteoglycan binding GO:0043394 ISO
    mercury ion binding GO:0045340 ISO
    receptor ligand activity GO:0048018 IDA
Biological Process
    angiogenesis GO:0001525 IEA
    regulation of protein phosphorylation GO:0001932 ISO
    acute-phase response GO:0006953 IEA
    cell-substrate junction assembly GO:0007044 IBA
    cell-substrate junction assembly GO:0007044 IDA
    cell adhesion GO:0007155 IDA
    cell-matrix adhesion GO:0007160 IBA
    cell-matrix adhesion GO:0007160 IDA
    cell-matrix adhesion GO:0007160 ISO
    calcium-independent cell-matrix adhesion GO:0007161 IDA
    integrin-mediated signaling pathway GO:0007229 IEA
    integrin-mediated signaling pathway GO:0007229 ISO
    nervous system development GO:0007399 IBA
    heart development GO:0007507 IBA
    positive regulation of cell population proliferation GO:0008284 ISO
    glial cell migration GO:0008347 ISO
    regulation of cell shape GO:0008360 IEA
    positive regulation of gene expression GO:0010628 IEA
    positive regulation of gene expression GO:0010628 ISO
    response to muscle activity GO:0014850 IDA
    peptide cross-linking GO:0018149 ISO
    extracellular matrix organization GO:0030198 ISO
    positive regulation of cell migration GO:0030335 ISO
    negative regulation of collagen biosynthetic process GO:0032966 ISO
    integrin activation GO:0033622 IEA
    integrin activation GO:0033622 ISO
    substrate adhesion-dependent cell spreading GO:0034446 IEA
    substrate adhesion-dependent cell spreading GO:0034446 ISO
    endodermal cell differentiation GO:0035987 IEA
    endodermal cell differentiation GO:0035987 ISO
    wound healing GO:0042060 IMP
    negative regulation of apoptotic process GO:0043066 ISO
    positive regulation of axon extension GO:0045773 IDA
    positive regulation of fibroblast proliferation GO:0048146 IEA
    positive regulation of fibroblast proliferation GO:0048146 ISO
    positive regulation of chemotaxis GO:0050921 ISO
    biological process involved in interaction with symbiont GO:0051702 IEA
    biological process involved in interaction with symbiont GO:0051702 ISO
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 ISO
    regulation of ERK1 and ERK2 cascade GO:0070372 IEA
    regulation of ERK1 and ERK2 cascade GO:0070372 ISO
    cellular response to mercury ion GO:0071288 ISO
    cellular response to interleukin-1 GO:0071347 ISO
    negative regulation of transforming growth factor beta production GO:0071635 IEA
    negative regulation of transforming growth factor beta production GO:0071635 ISO
    negative regulation of monocyte activation GO:0150102 IDA
    negative regulation of monocyte activation GO:0150102 ISO
    neural crest cell migration involved in autonomic nervous system development GO:1901166 ISO
    positive regulation of substrate-dependent cell migration, cell attachment to substrate GO:1904237 IEA
    positive regulation of substrate-dependent cell migration, cell attachment to substrate GO:1904237 ISO
Subcellular Localization
    fibrinogen complex GO:0005577 IEA
    fibrinogen complex GO:0005577 ISO
    basement membrane GO:0005604 IDA
    basement membrane GO:0005604 ISO
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IEA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 ISO
    apical plasma membrane GO:0016324 IDA
    extracellular matrix GO:0031012 IDA
    extracellular matrix GO:0031012 ISO
    extracellular matrix GO:0031012 TAS
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IDA
    collagen-containing extracellular matrix GO:0062023 ISO
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Fn1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Fn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Stxbp3 20912
Co-fractionation Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 Rab35 77407
Co-fractionation Mus musculus
4 Mep1b 17288
Co-fractionation Mus musculus
5 Mrps34  
Co-fractionation Mus musculus
6 Eed  
Affinity Capture-MS Mus musculus
7 Bzw2  
Co-fractionation Mus musculus
8 Tfe3  
Affinity Capture-MS Mus musculus
9 Fn1 14268
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
10 Col7a1  
Co-fractionation Mus musculus
11 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Fn1 is involved
PathwayEvidenceSource
Cell surface interactions at the vascular wall IEA Reactome
Cellular responses to mechanical stimuli IEA Reactome
Cellular responses to stimuli IEA Reactome
Degradation of the extracellular matrix IEA Reactome
ECM proteoglycans IEA Reactome
Elastic fibre formation IEA Reactome
Extracellular matrix organization IEA Reactome
Fibronectin matrix formation IEA Reactome
G alpha (s) signalling events IEA Reactome
GPCR downstream signalling IEA Reactome
GPER1 signaling IEA Reactome
GRB2:SOS provides linkage to MAPK signaling for Integrins IEA Reactome
Hemostasis IEA Reactome
Integrin cell surface interactions IEA Reactome
Integrin signaling IEA Reactome
MAP2K and MAPK activation IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
MET activates PTK2 signaling IEA Reactome
MET promotes cell motility IEA Reactome
Metabolism of proteins IEA Reactome
Molecules associated with elastic fibres IEA Reactome
Non-integrin membrane-ECM interactions IEA Reactome
p130Cas linkage to MAPK signaling for integrins IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
Platelet Aggregation (Plug Formation) IEA Reactome
Platelet degranulation IEA Reactome
Post-translational protein modification IEA Reactome
Post-translational protein phosphorylation IEA Reactome
RAF/MAP kinase cascade IEA Reactome
Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) IEA Reactome
Response of endothelial cells to shear stress IEA Reactome
Response to elevated platelet cytosolic Ca2+ IEA Reactome
Signal Transduction IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by MET IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Syndecan interactions IEA Reactome
Turbulent (oscillatory, disturbed) flow shear stress activates signaling by PIEZO1 and integrins in endothelial cells IEA Reactome





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