Gene description for Fn1
Gene name fibronectin 1
Gene symbol Fn1
Other names/aliases E330027I09
Fn
Fn-1
Species Mus musculus
 Database cross references - Fn1
ExoCarta ExoCarta_14268
Vesiclepedia VP_14268
Entrez Gene 14268
UniProt P11276  
 Fn1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Fn1
Molecular Function
    protein binding GO:0005515 IPI
    protease binding GO:0002020 ISO
    heparin binding GO:0008201 IEA
    peptidase activator activity GO:0016504 IDA
    integrin binding GO:0005178 ISO
    mercury ion binding GO:0045340 ISO
Biological Process
    wound healing GO:0042060 IMP
    positive regulation of cell migration GO:0030335 ISO
    calcium-independent cell-matrix adhesion GO:0007161 IDA
    negative regulation of apoptotic process GO:0043066 ISO
    cell adhesion GO:0007155 IDA
    cellular response to mercury ion GO:0071288 ISO
    extracellular matrix organization GO:0030198 ISO
    cell-matrix adhesion GO:0007160 ISO
    endodermal cell differentiation GO:0035987 ISO
    regulation of cell shape GO:0008360 IEA
    cell activation GO:0001775 ISO
    positive regulation of peptidase activity GO:0010952 IDA
    acute-phase response GO:0006953 IEA
    peptide cross-linking GO:0018149 ISO
    integrin activation GO:0033622 ISO
    angiogenesis GO:0001525 IEA
    positive regulation of axon extension GO:0045773 IDA
    glial cell migration GO:0008347 ISO
    positive regulation of chemotaxis GO:0050921 ISO
    cell-substrate junction assembly GO:0007044 IDA
    cellular response to interleukin-1 GO:0071347 ISO
    substrate adhesion-dependent cell spreading GO:0034446 ISO
Subcellular Localization
    proteinaceous extracellular matrix GO:0005578 TAS
    basement membrane GO:0005604 ISO
    basal lamina GO:0005605 IDA
    extracellular space GO:0005615 ISO
    extracellular exosome GO:0070062 ISO
    fibrinogen complex GO:0005577 ISO
    extracellular matrix GO:0031012 ISO
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 ISO
    extracellular region GO:0005576 IEA
    blood microparticle GO:0072562 ISO
    apical plasma membrane GO:0016324 IDA
 Experiment description of studies that identified Fn1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 210
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD81|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Fn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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