Gene description for Rab35
Gene name RAB35, member RAS oncogene family
Gene symbol Rab35
Other names/aliases 9530019H02Rik
AU040256
H-ray
RAB1C
RAY
Species Mus musculus
 Database cross references - Rab35
ExoCarta ExoCarta_77407
Vesiclepedia VP_77407
Entrez Gene 77407
UniProt Q6PHN9  
 Rab35 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rab35
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    GTPase activity GO:0003924 ISO
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 ISO
    GTP binding GO:0005525 ISS
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IEA
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 ISO
    GDP binding GO:0019003 ISO
    GDP binding GO:0019003 ISS
Biological Process
    mitotic cytokinesis GO:0000281 IEA
    mitotic cytokinesis GO:0000281 ISO
    protein localization GO:0008104 ISO
    protein transport GO:0015031 IEA
    endosomal transport GO:0016197 ISO
    endosomal transport GO:0016197 ISS
    antigen processing and presentation GO:0019882 IEA
    antigen processing and presentation GO:0019882 ISO
    neuron projection development GO:0031175 IMP
    endocytic recycling GO:0032456 IBA
    Rab protein signal transduction GO:0032482 IEA
    protein localization to endosome GO:0036010 ISO
    protein localization to endosome GO:0036010 ISS
    plasma membrane to endosome transport GO:0048227 IEA
    plasma membrane to endosome transport GO:0048227 ISO
    cellular response to nerve growth factor stimulus GO:1990090 ISO
    cellular response to nerve growth factor stimulus GO:1990090 ISS
Subcellular Localization
    mitochondrion GO:0005739 HDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    clathrin-coated pit GO:0005905 IEA
    clathrin-coated pit GO:0005905 ISO
    endosome membrane GO:0010008 IBA
    endosome membrane GO:0010008 ISO
    endosome membrane GO:0010008 ISS
    synaptic vesicle membrane GO:0030672 ISO
    synaptic vesicle membrane GO:0030672 ISO
    cell projection membrane GO:0031253 IEA
    cell projection membrane GO:0031253 ISO
    melanosome GO:0042470 IEA
    intercellular bridge GO:0045171 IEA
    intercellular bridge GO:0045171 ISO
    clathrin-coated endocytic vesicle GO:0045334 IEA
    clathrin-coated endocytic vesicle GO:0045334 ISO
 Experiment description of studies that identified Rab35 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rab35
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Set 56086
Affinity Capture-MS Mus musculus
3 Fn1 14268
Co-fractionation Mus musculus
View the network image/svg+xml



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