Gene description for Gna13
Gene name guanine nucleotide binding protein, alpha 13
Gene symbol Gna13
Other names/aliases AU024132
AU043124
Galpha13
Species Mus musculus
 Database cross references - Gna13
ExoCarta ExoCarta_14674
Vesiclepedia VP_14674
Entrez Gene 14674
UniProt P27601  
 Gna13 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Mov neuroglial cells 15210972    
Oligodendrocytes 21136642    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gna13
Molecular Function
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    D5 dopamine receptor binding GO:0031752 ISO
    type 1 angiotensin receptor binding GO:0031702 ISO
    GTP binding GO:0005525 IEA
    GTPase activity GO:0003924 TAS
    protein binding GO:0005515 IPI
    signal transducer activity GO:0004871 IBA
    metal ion binding GO:0046872 IEA
    guanyl nucleotide binding GO:0019001 IEA
    G-protein coupled receptor binding GO:0001664 ISO
    nucleotide binding GO:0000166 IEA
Biological Process
    adenylate cyclase-activating G-protein coupled receptor signaling pathway GO:0007189 IGI
    patterning of blood vessels GO:0001569 IMP
    activation of phospholipase D activity GO:0031584 ISO
    cell differentiation GO:0030154 IDA
    small GTPase mediated signal transduction GO:0007264 IEA
    intracellular signal transduction GO:0035556 IDA
    G-protein coupled receptor signaling pathway GO:0007186 TAS
    platelet activation GO:0030168 IMP
    signal transduction GO:0007165 IBA
    angiogenesis GO:0001525 IMP
    positive regulation of cytosolic calcium ion concentration GO:0007204 ISO
    in utero embryonic development GO:0001701 IGI
    regulation of cell shape GO:0008360 IDA
    Rho protein signal transduction GO:0007266 IDA
    regulation of cell migration GO:0030334 IMP
Subcellular Localization
    membrane GO:0016020 ISO
    nucleus GO:0005634 IEA
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 IEA
    cytosol GO:0005829 ISO
    intracellular GO:0005622 IDA
    brush border membrane GO:0031526 ISO
    heterotrimeric G-protein complex GO:0005834 TAS
    focal adhesion GO:0005925 ISO
 Experiment description of studies that identified Gna13 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 39
MISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|HSC70|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 33
MISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
5
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gna13
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TGFBR1 7046
Affinity Capture-Luminescence Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here