Gene description for Dnaja1
Gene name DnaJ (Hsp40) homolog, subfamily A, member 1
Gene symbol Dnaja1
Other names/aliases Hsj2
Nedd7
Species Mus musculus
 Database cross references - Dnaja1
ExoCarta ExoCarta_15502
Entrez Gene 15502
UniProt P63037  
 Dnaja1 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Dnaja1
Molecular Function
    chaperone binding GO:0051087 ISO
    C3HC4-type RING finger domain binding GO:0055131 ISO
    metal ion binding GO:0046872 IEA
    unfolded protein binding GO:0051082 IBA
    low-density lipoprotein particle receptor binding GO:0050750 ISO
    Hsp70 protein binding GO:0030544 ISO
    heat shock protein binding GO:0031072 IEA
    ATP binding GO:0005524 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    G-protein coupled receptor binding GO:0001664 ISO
Biological Process
    DNA damage response, detection of DNA damage GO:0042769 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    protein localization to mitochondrion GO:0070585 ISO
    sperm motility GO:0030317 IMP
    positive regulation of apoptotic process GO:0043065 ISO
    negative regulation of protein ubiquitination GO:0031397 ISO
    response to heat GO:0009408 IEA
    toxin transport GO:1901998 IMP
    protein refolding GO:0042026 IBA
    regulation of protein transport GO:0051223 ISO
    negative regulation of JUN kinase activity GO:0043508 ISO
    androgen receptor signaling pathway GO:0030521 IMP
    protein folding GO:0006457 IEA
    spermatogenesis GO:0007283 IMP
Subcellular Localization
    cytoplasm GO:0005737 IEA
    cytosol GO:0005829 ISO
    ubiquitin ligase complex GO:0000151 ISO
    mitochondrion GO:0005739 IEA
    endoplasmic reticulum GO:0005783 IEA
    intracellular membrane-bounded organelle GO:0043231 IEA
    extracellular exosome GO:0070062 ISO
    membrane GO:0016020 ISO
    nucleus GO:0005634 IEA
 Experiment description of studies that identified Dnaja1 in exosomes
1
Experiment ID 214
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Dnaja1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Dnaja1 is involved
No pathways found





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