Gene description for Kpnb1
Gene name karyopherin (importin) beta 1
Gene symbol Kpnb1
Other names/aliases AA409963
IPOB
Impnb
Species Mus musculus
 Database cross references - Kpnb1
ExoCarta ExoCarta_16211
Vesiclepedia VP_16211
Entrez Gene 16211
UniProt P70168  
 Kpnb1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Kpnb1
Molecular Function
    protein binding GO:0005515 IPI
    nuclear localization sequence binding GO:0008139 IBA
    kinesin binding GO:0019894 ISO
    enzyme binding GO:0019899 ISO
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    small GTPase binding GO:0031267 IEA
    protein-containing complex binding GO:0044877 ISO
    Hsp90 protein binding GO:0051879 IDA
    nuclear import signal receptor activity GO:0061608 IBA
    nuclear import signal receptor activity GO:0061608 IDA
    nuclear import signal receptor activity GO:0061608 ISO
    importin-alpha family protein binding GO:0061676 IPI
Biological Process
    RNA import into nucleus GO:0006404 ISO
    protein import into nucleus GO:0006606 IBA
    protein import into nucleus GO:0006606 IDA
    protein import into nucleus GO:0006606 ISO
    protein import into nucleus GO:0006606 ISO
    protein import into nucleus GO:0006606 ISS
    NLS-bearing protein import into nucleus GO:0006607 IEA
    NLS-bearing protein import into nucleus GO:0006607 ISO
    ribosomal protein import into nucleus GO:0006610 IDA
    ribosomal protein import into nucleus GO:0006610 ISO
    mitotic chromosome movement towards spindle pole GO:0007079 IEA
    mitotic chromosome movement towards spindle pole GO:0007079 ISO
    mitotic metaphase chromosome alignment GO:0007080 IEA
    mitotic metaphase chromosome alignment GO:0007080 ISO
    astral microtubule organization GO:0030953 IEA
    astral microtubule organization GO:0030953 ISO
    establishment of mitotic spindle localization GO:0040001 IEA
    establishment of mitotic spindle localization GO:0040001 ISO
    mitotic spindle assembly GO:0090307 IEA
    mitotic spindle assembly GO:0090307 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nuclear envelope GO:0005635 ISO
    nuclear pore GO:0005643 ISO
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 NAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 NAS
    cytoplasmic stress granule GO:0010494 IDA
    nuclear membrane GO:0031965 IEA
    nuclear membrane GO:0031965 ISO
    protein-containing complex GO:0032991 IDA
    NLS-dependent protein nuclear import complex GO:0042564 ISO
    endoplasmic reticulum tubular network GO:0071782 IEA
    endoplasmic reticulum tubular network GO:0071782 ISO
 Experiment description of studies that identified Kpnb1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
7
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Kpnb1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Fxr1 14359
Affinity Capture-MS Mus musculus
3 Nr3c1  
Affinity Capture-Western Mus musculus
4 Snapin  
Co-fractionation Mus musculus
5 Nxf1  
Co-fractionation Mus musculus
6 Kpna2 16647
Co-fractionation Mus musculus
7 KPNB1 3837
Affinity Capture-MS Homo sapiens
8 Fmr1  
Affinity Capture-MS Mus musculus
9 Cebpa  
Affinity Capture-MS Mus musculus
10 Rcc1  
Co-fractionation Mus musculus
11 Ipo7 233726
Co-fractionation Mus musculus
12 Sox9  
Reconstituted Complex Mus musculus
13 Gli1  
Affinity Capture-Luminescence Mus musculus
14 Iqcb1  
Affinity Capture-MS Mus musculus
15 Mettl21e  
Affinity Capture-MS Mus musculus
16 Hsp90aa1 15519
Affinity Capture-Western Mus musculus
17 Fzr1  
Affinity Capture-MS Mus musculus
18 Unk  
Affinity Capture-RNA Mus musculus
19 Nanog  
Affinity Capture-MS Mus musculus
20 Smndc1  
Co-fractionation Mus musculus
21 Eed  
Affinity Capture-MS Mus musculus
22 Atxn1  
Affinity Capture-MS Mus musculus
23 Fancd2  
Affinity Capture-MS Mus musculus
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