Gene description for DPYD
Gene name dihydropyrimidine dehydrogenase
Gene symbol DPYD
Other names/aliases DHP
DHPDHASE
DPD
Species Homo sapiens
 Database cross references - DPYD
ExoCarta ExoCarta_1806
Vesiclepedia VP_1806
Entrez Gene 1806
HGNC 3012
MIM 612779
UniProt Q12882  
 DPYD identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for DPYD
Molecular Function
    uracil binding GO:0002058 IBA
    protein binding GO:0005515 IPI
    dihydropyrimidine dehydrogenase (NADP+) activity GO:0017113 IBA
    dihydropyrimidine dehydrogenase (NADP+) activity GO:0017113 IDA
    dihydropyrimidine dehydrogenase (NADP+) activity GO:0017113 IMP
    dihydropyrimidine dehydrogenase (NADP+) activity GO:0017113 ISS
    protein homodimerization activity GO:0042803 IDA
    protein homodimerization activity GO:0042803 ISS
    metal ion binding GO:0046872 IEA
    flavin adenine dinucleotide binding GO:0050660 ISS
    NADP binding GO:0050661 IBA
    NADP binding GO:0050661 ISS
    4 iron, 4 sulfur cluster binding GO:0051539 IEA
Biological Process
    purine nucleobase catabolic process GO:0006145 IMP
    pyrimidine nucleobase catabolic process GO:0006208 IMP
    pyrimidine nucleobase catabolic process GO:0006208 ISS
    thymine catabolic process GO:0006210 IBA
    thymine catabolic process GO:0006210 IDA
    uracil catabolic process GO:0006212 IBA
    uracil catabolic process GO:0006212 IDA
    thymidine catabolic process GO:0006214 IDA
    CMP catabolic process GO:0006248 IEA
    dCMP catabolic process GO:0006249 IEA
    beta-alanine biosynthetic process GO:0019483 IEA
    UMP catabolic process GO:0046050 IEA
    dUMP catabolic process GO:0046079 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified DPYD in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DPYD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPE  
Co-fractionation Homo sapiens
2 APOA1BP 128240
Co-fractionation Homo sapiens
3 CHAC2 494143
Co-fractionation Homo sapiens
4 C9orf64 84267
Co-fractionation Homo sapiens
5 TRNT1 51095
Co-fractionation Homo sapiens
6 GAPDH 2597
Co-fractionation Homo sapiens
7 LXN 56925
Two-hybrid Homo sapiens
8 GOPC 57120
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
9 DDX58 23586
Affinity Capture-RNA Homo sapiens
View the network image/svg+xml
 Pathways in which DPYD is involved
PathwayEvidenceSource
Metabolism TAS Reactome
Metabolism of nucleotides TAS Reactome
Nucleotide catabolism TAS Reactome
Pyrimidine catabolism TAS Reactome





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