Experiment description of studies that identified Nme2 in exosomes

1 Experiment ID 907 MISEV standards ✔ EM EV Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a EV Enriched markers ✔ HSPA5 EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S. Journal name Proteomics Publication year 2023 Sample Colon cancer cells Sample name C26 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 210 MISEV standards ✔ EM EV Biophysical techniques ✔ CD81|FLOT1 EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23260141     Organism Mus musculus Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration. Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL. Journal name Cell Publication year 2012 Sample Fibroblasts Sample name Normal-Fibroblasts (L cells) Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 15 MISEV standards ✔ EM EV Biophysical techniques ✔ CD63 EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule mrna Identification method Microarray PubMed ID 17486113     Organism Mus musculus Homo sapiens Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO Journal name NCB Publication year 2007 Sample Mast cells Sample name MC9 Bone marrow-derived mast cells HMC-1 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.11-1.21 g/mL Molecules identified in the study Protein mRNA miRNA Methods used in the study Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array

4 Experiment ID 33 MISEV standards ✔ EM|IEM EV Biophysical techniques ✔ Alix|TSG101 EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21136642     Organism Mus musculus Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons? Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J Journal name PROTEOMICS_CL Publication year 2007 Sample Oligodendrocytes Sample name Oligodendrocytes Oli-neu Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.10-1.14 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [QTOF] Western blotting Immunoelectron Microscopy

5 Experiment ID 908 MISEV standards ✔ EM EV Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a EV Enriched markers ✔ HSPA5 EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S. Journal name Proteomics Publication year 2023 Sample T-cell lymphoma cells Sample name EL4 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for Nme2

Protein Interactor ExoCarta ID Identification method PubMed Species No interactions are found.

Pathways in which Nme2 is involved

No pathways found