Gene description for Rab1
Gene name RAB1, member RAS oncogene family
Gene symbol Rab1
Other names/aliases Gtbp
Rab-1
Rab1A
Ypt1
mKIAA3012
Species Mus musculus
 Database cross references - Rab1
ExoCarta ExoCarta_19324
Entrez Gene 19324
UniProt P62821  
 Rab1 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mov neuroglial cells 15210972    
Pancreatic cells 19351151    
 Gene ontology annotations for Rab1
Molecular Function
    GTP binding GO:0005525 ISO
    GTPase activity GO:0003924 ISO
    protein binding GO:0005515 IPI
    nucleotide binding GO:0000166 IEA
Biological Process
    defense response to bacterium GO:0042742 ISO
    growth hormone secretion GO:0030252 ISO
    Golgi organization GO:0007030 ISO
    vesicle transport along microtubule GO:0047496 ISO
    cell migration GO:0016477 ISO
    substrate adhesion-dependent cell spreading GO:0034446 IMP
    endocytosis GO:0006897 ISO
    transport GO:0006810 IEA
    melanosome transport GO:0032402 IMP
    small GTPase mediated signal transduction GO:0007264 IEA
    positive regulation of glycoprotein metabolic process GO:1903020 ISO
    virion assembly GO:0019068 ISO
    intracellular protein transport GO:0006886 TAS
    ER to Golgi vesicle-mediated transport GO:0006888 ISO
    interleukin-8 secretion GO:0072606 ISO
    autophagosome assembly GO:0000045 ISO
    vesicle-mediated transport GO:0016192 IEA
    cargo loading into COPII-coated vesicle GO:0090110 ISO
    protein transport GO:0015031 IEA
    autophagy GO:0006914 ISO
    Rab protein signal transduction GO:0032482 IBA
Subcellular Localization
    cell GO:0005623 IMP
    endoplasmic reticulum GO:0005783 IEA
    endosome GO:0005768 IEA
    extracellular exosome GO:0070062 ISO
    membrane GO:0016020 IEA
    neuronal cell body GO:0043025 ISO
    Golgi membrane GO:0000139 ISO
    endoplasmic reticulum membrane GO:0005789 ISO
    melanosome GO:0042470 IDA
    early endosome GO:0005769 ISO
    intracellular GO:0005622 ISA
    Golgi apparatus GO:0005794 ISO
    cytoplasm GO:0005737 ISA
 Experiment description of studies that identified Rab1 in exosomes
1
Experiment ID 214
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 39
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|HSC70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
5
Experiment ID 188
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Rab1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rab1 is involved
PathwayEvidenceSource
Golgi Cisternae Pericentriolar Stack Reorganization IEA Reactome





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